Filtering hgh

@Ghoul it’s possible you use a higher quality PES membrane with a more thorough treatment to reduce protein binding.

Coincidentally I just happened to already be using the same filter used for that specific test. Pall Pharmassure, the filter of choice in compounding pharmacies.

It's PES material, specifically the high performance branded type called "Supor", which is in a few filter brands. Pall and Braun both use it.

However, it's not significantly different from any other good brand of filter. Besides. .22um pores are over 50x times wider than the size of an rHGH monomer. So you're not exactly shoving it through an opening that's too small.

Finally, all peptide drugs, including rHGH, are sterilized by .2um filtration as a final step in manufacturing. Often more than once, during the entire process. So that should allay any concerns about filter damage.

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Coincidentally I just happened to already be using the same filter used for that specific test. Pall Pharmassure, the filter of choice in compounding pharmacies.

It's PES material, specifically the high performance branded type called "Supor", which is in a few filter brands. Pall and Braun both use it.

However, it's not significantly different from any other good brand of filter. Besides. .22um pores are over 50x times wider than the size of an rHGH monomer. So you're not exactly shoving it through an opening that's too small.

Finally, all peptide drugs, including rHGH, are sterilized by .2um filtration as a final step in manufacturing. Often more than once, during the entire process. So that should allay any concerns about filter damage.

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All PES is not made equal. In fact before it is treated, it is hydrophobic and therefore highly protein binding and unsuitable.

Also, it’s more about hydrophobic interactions mixed with shear rather than the literal sizes of the pores.

The surfactant is able to ameliorate this issue by occupying hydrophobic sites.

I wasn’t saying that filtering was an issue btw, just pointing out a reason why you might be having success filtering your hgh while I wasn’t with my cobetter pes membranes.

Likely the hydrophilic treatment is of a superior quality in those Supor membranes.
 
All PES is not made equal. In fact before it is treated, it is hydrophobic and therefore highly protein binding and unsuitable.

Also, it’s more about hydrophobic interactions mixed with shear rather than the literal sizes of the pores.

The surfactant is able to ameliorate this issue by occupying hydrophobic sites.

I wasn’t saying that filtering was an issue btw, just pointing out a reason why you might be having success filtering your hgh while I wasn’t with my cobetter pes membranes.

Likely the hydrophilic treatment is of a superior quality in those Supor membranes.
I use the cheap ass sterile aliexpress ones and have zero issues, just as a datapoint.
 
All PES is not made equal. In fact before it is treated, it is hydrophobic and therefore highly protein binding and unsuitable.

Also, it’s more about hydrophobic interactions mixed with shear rather than the literal sizes of the pores.

The surfactant is able to ameliorate this issue by occupying hydrophobic sites.

I wasn’t saying that filtering was an issue btw, just pointing out a reason why you might be having success filtering your hgh while I wasn’t with my cobetter pes membranes.

Likely the hydrophilic treatment is of a superior quality in those Supor membranes.

That's why I specified "good" brands. I would've expected Cobetter to fall into that group though. They're not a no-name filter company, but a fairly big player that's been around for 20 years.

They even specify they use their own proprietary technology for a "premium" PES filter member.

 
That's why I specified "good" brands. I would've expected Cobetter to fall into that group though. They're not a no-name filter company, but a fairly big player that's been around for 20 years.

They even specify they use their own proprietary technology for a "premium" PES filter member.

I’m just reasoning backwards due to assuming you aren’t having issues and I am, for example you aren’t noticing it clogs your 13mm filters easily?

There are other explanations though like maybe the hgh batches we are using are different etc.

I am pretty sure it’s due to differences in surfactant inclusion though…

As when I added the ps20 to the ssa hgh suddenly I could filter it in one go without issues.
 
What ps20 are you using?
I did look into this for awhile but could not find a USP grade or better source without breaking the bank. Was actually hoping to use it for tesa, not hgh tho.

HPBCD and cyclodextrin too, could not find a reliable source lol
 
Worth noting this is not refined or tested for toxins and not intended for biological applications. I did contact them to query.
It has a coa for heavy metals and It’s below the limits. What tells you its not refined?

Seems like it would need to be refined to meet the specifications here:
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This is general/industrial grade? Endotoxin, microbial, elemental values? The listed values don't conform to pharm grade at all. The company specifically told me it was not a pharm grade product when they sent the above information.
It has a coa for heavy metals and It’s below the limits. What tells you its not refined?

Seems like it would need to be refined to meet the specifications here:
 
What ps20 are you using?
I did look into this for awhile but could not find a USP grade or better source without breaking the bank. Was actually hoping to use it for tesa, not hgh tho.

HPBCD and cyclodextrin too, could not find a reliable source lol
Did you ever find a spot for bulk cyclodextrin? Or were you joking? Curious thanks
 
Did you ever find a spot for bulk cyclodextrin? Or were you joking? Curious thanks

Finding it is easy (cheap too), i just couldn't find one that's USP rated or whereabouts..I also don't want to spend 200$ testing it..and god knows what impurities are in it..
 
This is general/industrial grade? Endotoxin, microbial, elemental values? The listed values don't conform to pharm grade at all. The company specifically told me it was not a pharm grade product when they sent the above information.
Endotoxin - I don’t mind it not being tested. A refined non-biological product will have negligible levels. The peptides and hgh itself don’t have endotoxin testing either.

Just because it doesn’t have the paperwork to make it pharma grade doesn’t mean it’s not pure. The documents indicate it’s a highly refined product and they are the same as the USP specifications for this product if you look them up (only missing endotoxin testing, microbial, assay and ID).

Of course I am making an assumption (a reasonable one I think) and by not paying for the assurance of legit pharma grade it’s on me if I’m wrong.

At the end of the day it’s about your risk tolerance. Maybe they are lying and this is a made up CoA, it’s actually full of trash. Doesn’t seem very likely to me though. Still a risk.
 
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Is it really saying it is worse than the competitor at being low protein binding? Maybe I’m misunderstanding the chart.

Their PVDF seems to be the protein champ.

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So I didnt really know about filtering oils aside from when using ampules, but it makes sense to me. I thought people only used them to try and remedy/save oils from a contaminant of sorts..or ampules like mentioned above. Filtering your product cant do any harm. It's already filtered from your "high quality" vendor right? so it should be ez-pz to run through again for an added layer of protection and peace of mind before shoving it into your body.

The search function was filled with a ton of filtering stuff, but it mainly seemed to be homebrew and stuff aside from a couple comments here or there.

What is the standard operating procedure for accomplishing this correctly?

I read a couple different filter sizes.. Is one better for oil, one better for water based or 13 all around?

Whats the call between filtering as your loading your pin or buying sterile vials and filtering the whole content to new vial at once? Guessing more product loss to filter space and more filters required.

Some dude was seriously butthurt in the thread history about this filtering stuff.. this must be much more complex and deeper than I understand it to be right... RIGHT?

p.s its always funny when your reading a thread going through back and forth argument, but one guy is already banned from the first post you read so you know who the clown is from the start
 
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On a scale of 1 to 100

1 being realistically harmful; 100 being bubble boy purity

Filtering is like going from 97 to 98 → "it wasn't toxic to begin with and it didn't get much less toxic"

I doubt it's going from realistically harmful to bubble boy purity otherwise we'd be hearing a lot of complaints
 
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