MyNameIsJeff
Member
I'll pay 1 cent each, but only if each one of them has been individually tested for endotoxins and sterility. Would not want to get my hands dirty
MESO-Rx
Anabolic Steroids
Janoshik Analytical laboratory testing services
- Thread starter janoshik
- Start date
What were the findings on the validation work? You now have a validated endotoxin recovery method with the oil matrix?
janoshik
Subscriber
Good news is yes.
Bad news is we have decided we won't be sharing the details, as it would most likely get stolen by our competitors. I won't be saving them hundreds of manhours of method development.
Unfortunately, other labs are stealing our ideas and IP left and right, thus such decisions come to play.
Thanks for sharing. Understood.
You shared some anyway just to give me a good tease.
Good man. Now splurge a little and get everyone some of those expensive autopipettors!
janoshik
Subscriber
Well, yeah, we tried to run some more samples for the statistics on Friday so we can have a discussion in the lab we used to subcontract on it all and well, basically, the OIL testing for endotoxin is so fucked up, we gave up on trying.
When running the analyses and crossreferencing them with substances in the sample:
Benzyl Alcohol too high? Bam, results fucked up.
No BB? Bam, recovery fucked up.
Too much BB? BAM, processing takes hours which fucks the method up.
Weird kind of oil? Guess what.
Tren? Needs additional blanks.
At this point it looks more like every sinlge concoction would need pretty much new method. Guess that's the reason why it's never ever tested for in the pharmacopoeias.
We will not be touching endotoxin testing in oils again effective immediately.
We are pretty sure our last results were pretty OK, but I can offer a refund for any oil test for endotoxin should anyone desire that. Please, do let me know.
I am not sure what you mean by false safety. We've tested > 250 endotoxin samples in past 20 days alone and while many of them were positive, none ever came close to dangerous levels of endotoxin. I think, with a peace in my heart, I can say that this is much less of a worry than sterility, which can be so-so in the peptides.
I don't think that was a good idea and it had been nothing but a net loss for us. Material costs in the thousands and manhours wasted are countless.
janoshik
Subscriber
Now, I mean, we could charge 800-1000 USD per oil sample to reflect the costs, but I don't think that would be viable for our clients and most importantly, I have been begged by my CSO to just not do it, as it's incredibly frustrating to have to redo the test ten times before we get stable results.
And I prefer my lab techs and CSO not frustrated.
Only thanks to them it's not me doing everything and going crazy / retired.
And I prefer my lab techs and CSO not frustrated.
Only thanks to them it's not me doing everything and going crazy / retired.
janoshik
Subscriber
I'd like to clarify - I don't think our AAS oil Endotoxin tests are bad - the issue is that each type of oil basically required us to create a new test for it.
We cannot use routine approach, as we have numbers flying left and right then.
We are a high throughput laboratory and we don't assume people to be willing to be paying 800-1000 USD for a test such as that, so we're dropping it.
On AAS oil Endotoxin testing we've underestimated the influences of the matrix in target recovery and it cost us many thousands of dollars and I've decided to cut the loss here, as we're not willing to run a test at a severe loss (what we've been doing) or send out numbers we're less than confident in.
We cannot use routine approach, as we have numbers flying left and right then.
We are a high throughput laboratory and we don't assume people to be willing to be paying 800-1000 USD for a test such as that, so we're dropping it.
On AAS oil Endotoxin testing we've underestimated the influences of the matrix in target recovery and it cost us many thousands of dollars and I've decided to cut the loss here, as we're not willing to run a test at a severe loss (what we've been doing) or send out numbers we're less than confident in.
Would it make sense to have some conditions on the oil to test like :
BA Should be between 0 and 5 %
BB Should be between 10 and 30 %
Oil should be MCT, Sesame, GSO, CSO
I wonder how compounding pharmacies are able to test the endotoxin levels (pretty sure that they must have different preparations)
janoshik
Subscriber
I don't think they test finished oils.
Or do they?
Also the above conditions would mean we have to run multiple tests before testing already.
Thanks very much for the updates. Quite a rabbit hole. Thank you for trying.
Sample Treatments That Solve Low Endotoxin Recovery Issues - PubMed
Dilution of samples with water (water dilution method) was not appropriate for measuring the endotoxin activity in solutions containing chelating agents and detergents-typical formulations showing low endotoxin recovery (LER). Dilution of the samples with 2 mM magnesium solution (magnesium...
pubmed.ncbi.nlm.nih.gov
Low Endotoxin Recovery- What does it mean? Why it is important to investigate? | FUJIFILM Wako Chemicals Europe GmbH
To ensure drug safety, it is imperative to thoroughly investigate LER and its potential implications. LER carries the possibility of false-negative results, where the presence of elevated endotoxin levels can go undetected, compromising quality control te
www.wako-pyrogen.com
Hi Jano,
So the reported "dimer" value for GH testing includes all DPn greater than DP1 (monomer)? Hence "dimer" on report is DP2, DP3, etc picked up by SEC?
TLDR: "dimer" value on your test reports are all "aggregates (DP2-DPn)" of GH larger than monomer?
Thank you.
For example:
Fig. 5 of attached.
Last edited:
janoshik
Subscriber
>dimer" value on your test reports are all "aggregates (DP2-DPn)" of GH larger than monomer?
Yep.
Yep.
I should have said "dimer and related proteins" as per your report nomenclature.
Thanks for confirming.
@janoshik
I had a few samples of things tested by your company (thanks!).
The Tadalafil came up at 68% with the rest unknown. I briefly considered trying to purify it myself, but realized this is impracticable because it does not readily dissolve and I am not a chemist to do anything more advanced than basic things.
But this got me curious to better understand how it is analyzed: I know you use methanol but even that is not supposed to dissolve tadalafil. My understanding is that HPLC requires a liquid analyte. How do you get tadalafil dissolved in order to analyze it? I appreciate your helping my understanding.
I had a few samples of things tested by your company (thanks!).
The Tadalafil came up at 68% with the rest unknown. I briefly considered trying to purify it myself, but realized this is impracticable because it does not readily dissolve and I am not a chemist to do anything more advanced than basic things.
But this got me curious to better understand how it is analyzed: I know you use methanol but even that is not supposed to dissolve tadalafil. My understanding is that HPLC requires a liquid analyte. How do you get tadalafil dissolved in order to analyze it? I appreciate your helping my understanding.
janoshik
Subscriber
Tadalafil is nicely soluble in methanol, you just have to use enough of it.
It's not a problem to do something like 1.8 mg/ml and that's plenty for analysing.
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