MyNameIsJeff
Member
I'll pay 1 cent each, but only if each one of them has been individually tested for endotoxins and sterility. Would not want to get my hands dirty... so how much per cap?

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I'll pay 1 cent each, but only if each one of them has been individually tested for endotoxins and sterility. Would not want to get my hands dirty... so how much per cap?
What were the findings on the validation work? You now have a validated endotoxin recovery method with the oil matrix?We expect results Monday - no kidding.
We had a delay because 1st test kit was bad, so we had to wait for replacement, then we spent 14 days validating the methods instead of anticipated 2-3 days and ran out of supplies.
Then the other kit was supposed to arrive Monday, but arrived yesterday afternoon.
Now today I was in no state to stand and pipette all day (endotoxin testing is heavy dependent on a lot of pipetting) because me and my middle son have pneumonia. And the only other person in the company who can run those tests by herself is on a holiday until tomorrow.
I apologize for the clusterfuck that is.
We can provide you with the results Monday and refund the tests too if you wish.
Good news is yes.What were the findings on the validation work? You now have a validated endotoxin recovery method with the oil matrix?
Thanks for sharing. Understood.Good news is yes.
Bad news is we have decided we won't be sharing the details, as it would most likely get stolen by our competitors. I won't be saving them hundreds of manhours of method development.
Unfortunately, other labs are stealing our ideas and IP left and right, thus such decisions come to play.
You shared some anyway just to give me a good tease.Good news is yes.
Bad news is we have decided we won't be sharing the details, as it would most likely get stolen by our competitors. I won't be saving them hundreds of manhours of method development.
Unfortunately, other labs are stealing our ideas and IP left and right, thus such decisions come to play.
The endotoxin result here should be thrown out given what we've learned now. There is reasonable suspicion this first Gen Endotoxin result was a false negative at or below LOD. I've asked @janoshik if he will support a credit for retesting (with all prior ET clients) now that he has brought the method in house with proper endotoxin recovery method from oil matrix.
Well, yeah, we tried to run some more samples for the statistics on Friday so we can have a discussion in the lab we used to subcontract on it all and well, basically, the OIL testing for endotoxin is so fucked up, we gave up on trying.The endotoxin result here should be thrown out given what we've learned now. There is reasonable suspicion this first Gen Endotoxin result was a false negative at or below LOD. I've asked @janoshik if he will support a credit for retesting (with all prior ET clients) now that he has brought the method in house with proper endotoxin recovery method from oil matrix.
When running the analyses and crossreferencing them with substances in the sample:
Benzyl Alcohol too high? Bam, results fucked up.
No BB? Bam, recovery fucked up.
Too much BB? BAM, processing takes hours which fucks the method up.
Weird kind of oil? Guess what.
Tren? Needs additional blanks.
I don't think they test finished oils.Would it make sense to have some conditions on the oil to test like :
BA Should be between 0 and 5 %
BB Should be between 10 and 30 %
Oil should be MCT, Sesame, GSO, CSO
I wonder how compounding pharmacies are able to test the endotoxin levels (pretty sure that they must have different preparations)
Hi Jano,yes, but I scratched what I don't really understand/doesn't make sense
Figure 5 shows
the size-exclusion chromatogram of
somatropin carried out under the same conditions as described previously.
The inset shows the zoomed baseline region where dimer and higher molecular
weight aggregates are evident.
I should have said "dimer and related proteins" as per your report nomenclature.>dimer" value on your test reports are all "aggregates (DP2-DPn)" of GH larger than monomer?
Yep.
Tadalafil is nicely soluble in methanol, you just have to use enough of it.@janoshik
I had a few samples of things tested by your company (thanks!).
The Tadalafil came up at 68% with the rest unknown. I briefly considered trying to purify it myself, but realized this is impracticable because it does not readily dissolve and I am not a chemist to do anything more advanced than basic things.
But this got me curious to better understand how it is analyzed: I know you use methanol but even that is not supposed to dissolve tadalafil. My understanding is that HPLC requires a liquid analyte. How do you get tadalafil dissolved in order to analyze it? I appreciate your helping my understanding.