janoshik
Subscriber
Very interesting.
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It does not really work that way with bioengineered products.It's "Gods" sample but apparently God MAY have overlooked the importance of including all the required Amino Acids, and much like not having an arm or leg such changes could effect FUNCTION.
There IS another possibility which is; Gods sample is not very "pure" and contains enough GH subunits or isoforms to skew the AAA data.
And an trypsin LC/MS is the most reliable means of differentiating those possibilities.
It's "Gods" sample but apparently God MAY have overlooked the importance of including all the required Amino Acids, and much like not having an arm or leg such changes could effect FUNCTION.
There IS another possibility which is; Gods sample is not very "pure" and contains enough GH subunits or isoforms to skew the AAA data.
And an trypsin LC/MS is the most reliable means of differentiating those possibilities.
Damn Jim no offense, but that's just another one of your "explainations" that worked, NOT!
Good to see that you are testin the testin, very fair.....Seems to be the case BUT I'm looking into having an LC/MS conducted on that sample for further confirmation.
It hasn't been updated yet.Can someone provide the link for this assay that has the underdosed serostim ?
Can someone provide the link for this assay that has the underdosed serostim ?
It's right in mands' post dude, come on the hell on... This thread is only two pages long!
SAMPLE --------- B (TESTED @ 2.08mgs)
SEROSTIM 4mg, Donated by a MESO member - Private Source (pharmacy)
View attachment 54523
SAMPLE ------- F-2 (TESTED @ 2.46mgs)
Haiziyuan BioHygene (8IU), Donated by a MESO member - Sport-HGH.com
-SIMEC TESTED @ 2.87mg/vial (Both vials tested are from different batches)
View attachment 54522
SAMPLE ---------- D (TESTED @ 4.32mgs - 13IUs)
AngTropin 3.33 mg, Donated by MESO member - Toptopshop.org
-SIMEC TESTED @ 17.5IUs/vial (Both vials tested are from same kit)
View attachment 54524
SAMPLE ---------- E-2 (TESTED @ 3.62mgs)
Hygetropin 3.33mg, Donated by a MESO member - WorldHGH.com Hygetropin.com.cn
-DIFFERENT LAB USING SAME (AAA) TESTING METHOD TESTED @ 2.832 mgs/vial
(Both vials tested are from same kit)
View attachment 54525
SAMPLE ---------- G (Tested @2.80mgs)
JINTROPIN 3.33mg, Donated by a MESO member -JINTROPIN-Online.com
-SIMEC TESTED @ 2.69mgs/vial
View attachment 54527
-DIFFERENT LAB USING SAME (AAA) TESTING METHOD TESTED @ 1.405mgs/vial
View attachment 54529
* NOTE (all vials tested are Authentic Jin's from different regions - Russia, Mexico,etc)
LAB STATES:
If the somatropin is a pure protein without formulation buffer AAA will allow quantification of the protein using our standard AAA.
However, if somatropin is formulated as a pharmaceutical drug for injection, a glycine buffer is used for the formulation. Therefore the glycine peak will be very large and comprise the data.
SANDOZ OMNITROPE (Generic) contains GLYCINE 27.6 mgs/vial
View attachment 54531
View attachment 54532
View attachment 54535
I have 4 more rHGH samples at my normal testing lab being tested using AAA testing method
I'll post those when I receive them......but.....it seems this isn't the most reliable testing method
GodTropin 24 IU - 5.87mg, Donated by a MESO member - godt240.com
mands
@ProfessorX did you do any recent testing on the 24iu vials of GodTropin ? I believe I saw an older test but noticed it was left out in your post above ... was that done on the old 12iu labeled vials?
@ProfessorX did you do any recent testing on the 24iu vials of GodTropin ? I believe I saw an older test but noticed it was left out in your post above ... was that done on the old 12iu labeled vials?
Professor X
Does that statement mean just Products that use a buffer solution( glycine)?
Or are you sayin that AAA testin in general isn't the most reliable testing method?????
@ProfessorX ah! Thanks for catching that ... fixed.
And I was asking if you recently tested the gods (not through Jim) ... curious to see if there was any variance to the result compared to these current reports.
Sorry..correction
I DO have a GodT sample vial from the same kit tested through Jim at a different Lab
Waiting on the results (AAA)
I'd like Jims take on GodTropin sample Z
There's been complaints about injection site irritation (red welts) with different GodT "batches"
Maybe there is a connection?
Buck,
I don't know enough about the testing to give an accurate answer, so I'll let Mands, Jim, and Jano discuss that.
But....judging by the inconsistent sample results posted, the SEROSTIM result, and the information stated by the lab....IMO....I think the results are hit and miss using this testing method (AAA) for the type of samples we are testing (formulated proteins - biosimilars)