A real environment

[Bumaga]

Lots of discusion about pictures of your lab.the brew process seems dangerously simple. My question is what would make for the perfect environment to create a home brew. I am a perfectionist. Testing end result is possible but why bother if the end ptoduct is anything short of a best effort.Clean is obvios but perfect lies in the smallest of details.

 

[Evom1]

Are you talking pre sealed sterile vials, or capping your own?

 

[Bumaga]

I am reading as fast as i can.The muscles in my thumbs are on point writing these questions on my phone I could challnge the hulk to a thumb wrestling match. I think I would go cap my own. i would want to sterilize everything directly before filling to be certain. Truth is I dont know shit yet but I am researching. Just want to do it right .

 

[LordSamuilo]

perfect environment to create a home brew.

The Perfect environment doesnt exsist inside a homebrew setting . Well atleast not at a discount . You can be extremely thorough and make a great product in a impromptu lab , but its never going to be 100% optimal without investing so much money that it becomes impractical to brew for personal use.

Useless its a business venture and your willing to invest a fat chunck of change to make a safe product , just stick to sterile sealed vials and syringe filters . Its the only way to ensure serility because everything stays self contained past the point of filtration .

Once you crossover to vacuum filtering and filling/transferring/capping your own vials , you to take relatively easy , safe and cheap process and punch a bunch of holes through it . Definitely not the route you should take when your just starting out .

 

[Bumaga]

Ok that makes sense. Yeah not trying to go big as a brewer just want to do it right.what about oils. How does a brewer know if the oils are free of any contaminants? Is there one brand better then another?

 

[LordSamuilo]

How does a brewer know if the oils are free of any contaminants?

You got some reading to do my man , but to answer your question anything that passes through your filter (.2 micron) will be contaminate free . There isnt any consensus as to which carrier is best , everbody has a different preference when it comes to carrier oil . gso or mct are the generally the go to oils you'll see most guys use though .

 

[Bumaga]

Yep will keep reading. Thanks for the info.

 

[ulfbehrt]

Lots of discusion about pictures of your lab.the brew process seems dangerously simple. My question is what would make for the perfect environment to create a home brew. I am a perfectionist. Testing end result is possible but why bother if the end ptoduct is anything short of a best effort.Clean is obvios but perfect lies in the smallest of details.

I just home brew my own personal with gloves and a mask and use sterile everything, but back when I use to home brew for friends as well I turned a 8x8 grow tent into a mini lab and it was fucking epic. Get a decent hepa filter and a inline fan and a LOT of iso alcohol and surgical gloves as well as a clean room suit. Also needed is a 3M respirator. If you want to go all out it's a few grand just to set up the mini lab. Here are some pics to help visualize.

 

[Bumaga]

That is what i was thinking would be real home setup. That must have been fun Doc. New to all this so will only brew for personal. Still gloves mask stetile everything and a empty clean room disinfected with clorox water solution sounds like a start. oh yeah and a hair net I got from the deli girl.

 

[master.on]

Lots of discusion about pictures of your lab.the brew process seems dangerously simple. My question is what would make for the perfect environment to create a home brew. I am a perfectionist. Testing end result is possible but why bother if the end ptoduct is anything short of a best effort.Clean is obvios but perfect lies in the smallest of details.

Glove box

You can even build one yourself

Sterile Glove Box - Mushroom Cultivation - Shroomery Message Board

as long as it's airtight and properly sterilized with alcohol or formaldehyde
it's actually cleaner than a pharma clean room.
Make sure all plastic and gloves are sterilizing-fluid resistant.

 

[Bumaga]

Glove box

You can even build one yourself

Sterile Glove Box - Mushroom Cultivation - Shroomery Message Board

as long as it's airtight and properly sterilized with alcohol or formaldehyde
it's actually cleaner than a pharma clean room.
Make sure all plastic and gloves are sterilizing-fluid resistant.

Funny thing is I have a huge fish tank in the garage. Thought about a sheet of lexan for the lid. I believe plumbing flages could work for the arm holes. I got stuck wondering how to vent it? If a hot plate was used inside of it the air inside would expand and need to have a way out. Still researching as much as I can about brewing. Starting to understand. New question read a lot about Chinese crap but everywhere so far that i have found raws they originate in China?

 

[Bumaga]

Not asking for a domestic source just wondering if this is rhe norm? Are. most domestic suppliers just selling chinese raws?

 

[RandallNowan]

Not asking for a domestic source just wondering if this is rhe norm? Are. most domestic suppliers just selling chinese raws?

I would say so - but it's just an opinion.

 

[Bumaga]

Well that just sucks. Nothing against China just wish i could keep my money in the U.S.A. All that being said Is the a chinese producer that is known to produce great Raws?

 

[master.on]

Funny thing is I have a huge fish tank in the garage. Thought about a sheet of lexan for the lid. I believe plumbing flages could work for the arm holes. I got stuck wondering how to vent it? If a hot plate was used inside of it the air inside would expand and need to have a way out. Still researching as much as I can about brewing. Starting to understand. New question read a lot about Chinese crap but everywhere so far that i have found raws they originate in China?

Aquariums are great start material for glove boxes

http://crystalbismuth.blogspot.com/p/glove-box-1-cheap.html

You don't really need to vent it, the hand movement (trough gloves) won't displace a lot of air
If you really want a vent port, you can drill a third hole in the gloves lexan panel, and attach a filter membrane to it either drilling some holes for support screws, gaskets and flange or just with duct tape on the outside.
Thus the filter membrane allows clean air to pass in/out as required, while keeping contaminants out

and you won't place the hot plate in it, just used for transferring gear from sterile media bottles (from vacuum filtration units) to sterile vials, while keeping proper sterility.

 

[Engineer]

Funny thing is I have a huge fish tank in the garage. Thought about a sheet of lexan for the lid. I believe plumbing flages could work for the arm holes. I got stuck wondering how to vent it? If a hot plate was used inside of it the air inside would expand and need to have a way out. Still researching as much as I can about brewing. Starting to understand. New question read a lot about Chinese crap but everywhere so far that i have found raws they originate in China?

Ideally your glovebox should be maintained in a negative pressure (i.e., a vacuum).

 

[Bumaga]

Ideally your glovebox should be maintained in a negative pressure (i.e., a vacuum).

Yeah i was wondering about that. Would that need to be a constant value that was held? I was thinking about a computer fan outside of a resperator filter that would constantly pull air from the box. Then putting a filtered vent on the opposite end. My thought was if the fan pulled more air then the vent allowed make up air it would create a vacum but also filter any air that was in the box before sealing it?

 

[RandallNowan]

That'll work.

 

[Engineer]

Yeah i was wondering about that. Would that need to be a constant value that was held? I was thinking about a computer fan outside of a resperator filter that would constantly pull air from the box. Then putting a filtered vent on the opposite end. My thought was if the fan pulled more air then the vent allowed make up air it would create a vacum but also filter any air that was in the box before sealing it?

To verify it's at a constant value, you would really need something to monitor differential pressure to verify that the vacuum was sufficient. That would be overkill in your case. Really all you need is for the pressure inside the box to be lower than atmospheric. I cant say for sure, but I highly doubt a computer fan would be able to provide sufficient vacuum. I would rig some 3" diameter duct work to the box (airtight sealed to the inside of the box) and a larger fan with some sort of filter (ideally a HEPA filter) to prevent debris from reaching the fan.

 

[Bumaga]

Makes sense. Kinda like projects that make think. Also like the idea of being self sufficient when it comes to all this. I dont know though. The more I look into it the more it seems like I am chasing the same demon so to speak. Lots af talk about Chinese products being bunk. The raws I have seen originate in China. At this point it seems like a person is just as likely to get bunk raws as it is to get bunk finished products. Either way it appears the only way to know is to go for it on kinda on faith and then test it before pinning it. Still knowledge never hurts and I am lacking in much of it here so thank you.