bigbusiness
New Member
You dont wanna degrade the oil. Hold the oil at 212 for 15, it will kill all microbial contaminants
MESO-Rx
Anabolic Steroids
Buchner Filter paper .22
- Thread starter Stinkfinger1112
- Start date
Stinkfinger1112
Member
Gotcha!
Before you accept this advice i would do some research for yourself. Even in autoclaves they use a higher temperature than 212 degrees, and they have the benefits of steam and pressure. If your willing to risk it, thats your call, but at least google dry heat sterilization of parenteral oil.
bigbusiness
New Member
Please be kind and share with everyone the research you've done. Because I would like to see these studies myself
Just google dry heat sterilization of parenteral oils. They show 150 degrees celsius for 1 hour to be sufficient to kill all bacteria. Could you get away with lower? Possibly, but i havent seen a study that shows 212 is sufficient dry heat to sterilize oils for parenteral use.
Here is a little bit of the study: Dry-heat sterilization of the three oils at 150 deg C for one hour appeared to be sufficient for time and temperature conditions. However, the authors recommend dry-heat sterilization procedures be validated for each product.
Now show me a study that states 212 degrees for 15 minutes is sufficient to sterilize parenteral oils.
Here is a little bit of the study: Dry-heat sterilization of the three oils at 150 deg C for one hour appeared to be sufficient for time and temperature conditions. However, the authors recommend dry-heat sterilization procedures be validated for each product.
Now show me a study that states 212 degrees for 15 minutes is sufficient to sterilize parenteral oils.
bigbusiness
New Member
100°C is enough to kill 99% of all microbial contaminants. Are you forgetting he is also filtering with a .22 filter? Which pretty much sterilizes the solution......the dry heat was just an extra precaution because he is using a buchner setup
100°c is the point that water boils. The point of which water boils also kills 99% of all microbes, the ones it doesnt kill are very rare and are in remote location were heat is something the organism is accustomed too. In which case the .22 filter would take care that.
100°c is the point that water boils. The point of which water boils also kills 99% of all microbes, the ones it doesnt kill are very rare and are in remote location were heat is something the organism is accustomed too. In which case the .22 filter would take care that.
jstarks11
New Member
Please next time skip all the bs and just use whatman polycaps. Vacuum filter into a sterile sealed media bottle and you do NOTHING else. The filters with the filter disc is not really preferred. Vacucaps I believe they are called are also decent. I have done alot with those with NO problem. Then the millipore nalgene type thing are okay. But a media bottle with vacuum barbed nipples and sterile oven baked materials. Run The setup with filter and tubing with some pure grain alcohol through it enough to soak the tubing and filter. Then leave it running for 15 min, all alcohol is dissolved. Now filter oil. Final product is contained in a sterile sealed environment. Put your sterile bottle top dispenser on top and pump away.
Stinkfinger1112
Member
Next time I am.
So you ask for a study i rpovide it, and you disregard it. Then you say 100°C is enough. The most common tools to sterilize things are autoclaves, and dry heat sterilizer both use temeratures higher than 100°C.
bigbusiness
New Member
And what's your point!! OP is asking about filtering with a buchner setup. Oh let me guess you wanna measure dicks huh!! Lmao if you wanna see my dick all you gotta do is ask brotha. This can go down in DM [emoji108]
Cakedup
Subscriber
Again this man is right. Like I said before brother it's nice to have someone around that knows his shit. @jstarks11 I was starting to think this whole process was rocket science.lol. I just think alot of people don't answer questions for new brewers or with each other like we use to years ago....
Stinkfinger1112
Member
After talking with @Cakedup this setup is ok. My main concern was if I needed to sterilize the filters them selves. ....I don’t. No bacteria can pass through .22 micron filter. I will just be extra careful and make sure the filter does not touch anything and goes straight into the Büchner funnel.
Cakedup
Subscriber
@stinkfinger also I know pre wetting the filter with BA is not going to properly sterilize it, but it does help and it's better than nothing. Also by pre wetting the filter and adding vacuum pressure before adding the oil to the funnel will seal the filter to the base of the bunchner funnel. Therefore also preventing any unfiltered oil from running under the filter and into the filtering flask. That a long with the sterilization process we went over you won't have any problems with bacteria. You won't have any problems with brewing and recipes if you use the hotline I gave you.. You got this brother...
Stinkfinger1112
Member
Gotcha big bro! Yeah we talked about how to seal the filter just never touched on if I needed to sterilize the paper but the consensus is that it’s not necessary.
Cakedup
Subscriber
I'm going to shoot you a message like bro..
jstarks11
New Member
Thanks bro. Yeah, it seems like the game has definately changed alot. Used to be that I would spend all my time on here helping bros and sharing techniques. Now you have to watch for the renegade bumping behind the screen and shit. I just want to pass knowledge and learn man. Been doing this for as long as I can remember. I'm not sure how old this board is, but I've been a member of older boards since way back. Anytime I can help that is what I come here for. Lot of haters today compared to the old days.... LOT of haters. Just look at the frequency of RATS now days. Used to (especially in the juice game) you had STAND UP guys. Now there is RATS everywhere you go!!!
superbane
Well-known Member
yes
Sterilize the filters in an autoclave
if you have any doubts email the manufacturer.
Sterilize the filters in an autoclave
if you have any doubts email the manufacturer.
superbane
Well-known Member
Are you getting this information from a majority of individuals whom actually use a Buchner setup with these type of filters?
sterilize the filters in an autoclave it is pretty simple and takes all of 15 minutes
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