Dirts Brew log

[narta]

dirt what about if I just cap it with a normal gl45 cap the media bottle and then when I need to move it into a vial I just open the cap, draw quickly into a big syringe and that's it? would that compromise sterility much if I don't use a cap with a stopper?

@narta

If the cap has been properly sterilized and has a silicone ring to make it airtight I see no problem

 

[Sampei]

If the cap has been properly sterilized and has a silicone ring to make it airtight I see no problem

isn't the cap coming with the media bottle filter set of ks-tek already sterilized?
my issue is if when I open the media bottle to suck the oil out with the syringe during that time it can create some sterility issue.

 

[narta]

isn't the cap coming with the media bottle filter set of ks-tek already sterilized?

Oh I thought about glass gl45 bottler. I wouldn't advise to store long term in the receiver flask being that they are plastic and there maybe a reaction with the solvents.

 

[Sampei]

Oh I thought about glass gl45 bottler. I wouldn't advise to store long term in the receiver flask being that they are plastic and there maybe a reaction with the solvents.

ok so let's assume I filter it using the top bottle system it goes into the sterile plastic bottle and then I draw instantly after filtering everything into a sterile syringe of like 60ml and transfer it into sterile vial, would that be ok?

I just don't want to have to sterilize fucking caps etc but I don't want to risk that my oil that has just been sterilized to become not sterile anymore while I do the transfer process.

 

[Ateam2023]

Oh I thought about glass gl45 bottler. I wouldn't advise to store long term in the receiver flask being that they are plastic and there maybe a reaction with the solvents.

wheres a good media bottle supplier? gl45 media bottles?

 

[Sampei]

wheres a good media bottle supplier? gl45 media bottles?

ks-tek you can find almost everything there.

 

[narta]

ok so let's assume I filter it using the top bottle system it goes into the sterile plastic bottle and then I draw instantly after filtering everything into a sterile syringe of like 60ml and transfer it into sterile vial, would that be ok?

Yes it will be fine

 

[narta]

wheres a good media bottle supplier? gl45 media bottles?

opt for high borosilicate glass ones that can withstand temps

 

[Sampei]

Yes it will be fine

Now any pump to suggest for the whole setup? Hand pump for brake bleeding is fine? Electrical vacuum pump?

 

[Sampei]

I'm making a fucking post with everything needed and where we can find it. So you guys can stop answering the same question every fucking time

I feel like an annoying mosquito

 

[narta]

Hand pump for brake bleeding is fine?

cheapest option but reliable for up to 500ml-1l I think

Electrical vacuum pump?

Opt for a oil free that doesn't need any maintenance. JoanLab has some good ones

 

[Sampei]

cheapest option but reliable for up to 500ml-1l I think

Opt for a oil free that doesn't need any maintenance. JoanLab has some good ones

What does it mean reliable up to 500ml-1l? That if I try to do more then that like 1.5l it will not be able to pull?

I ain't gonna do more then a 500ml probably ever so no big deal if I understood correctly.

What's the main difference between electrical or hand pump? I mean hand pump it's not like you gotta keep pumping right? You set and forget?

 

[narta]

What does it mean reliable up to 500ml-1l? That if I try to do more then that like 1.5l it will not be able to pull?

I ain't gonna do more then a 500ml probably ever so no big deal if I understood correctly.

What's the main difference between electrical or hand pump? I mean hand pump it's not like you gotta keep pumping right? You set and forget?

the electrical pump can be fine tuned to pump at a certain rate aka holding pressure constant. With a hand pump you need to manually adjust that

 

[narta]

If one needs to go bigger/faster he should opt for a capsule in-line filter and a peristaltic pump. Cheaper in the long run, all-in-one system with fewer moving parts and all self contained

 

[Dirthand]

What does it mean reliable up to 500ml-1l? That if I try to do more then that like 1.5l it will not be able to pull?

I ain't gonna do more then a 500ml probably ever so no big deal if I understood correctly.

What's the main difference between electrical or hand pump? I mean hand pump it's not like you gotta keep pumping right? You set and forget?

Yes you have to give it a couple pumps every so often..

 

[Sampei]

If one needs to go bigger/faster he should opt for a capsule in-line filter and a peristaltic pump. Cheaper in the long run, all-in-one system with fewer moving parts and all self contained

Will not need that, I'm not selling and just doing it for me and maybe one two friend, period but I'll check it out, like to see and learn new things

 

[Spaceman Spiff]

Does anyone have found vacuum filter top system with PTFE membrane? I can't find it, only PES or NYLON wtf
BA is not compatible with PES for example, PTFE is the most compatible and best filter for what I have gathered reading the compatibility charts but I can't find any bottle top filter vacuum system that use it, why??

@narta @Spaceman Spiff @dirthand

I dont use those.

 

[nightprowler7]

Anyone know of a higher quality gl45 Corning septa than the ptfe ones on Amazon/ebay? Those fuckers are so thick you can barely get a 21g through it. It really doesn’t seem like they are made to be pierced.

 

[Dirthand]

Anyone know of a higher quality gl45 Corning septa than the ptfe ones on Amazon/ebay? Those fuckers are so thick you can barely get a 21g through it. It really doesn’t seem like they are made to be pierced.

Post 519 this thread. I haven't used any but have been wanting to get some

 

[AlexDavis43]

Anyone know of a higher quality gl45 Corning septa than the ptfe ones on Amazon/ebay? Those fuckers are so thick you can barely get a 21g through it. It really doesn’t seem like they are made to be pierced.

Post 519 this thread. I haven't used any but have been wanting to get some

Like I said I'm bored tonight so here ya go: post 519 and the AliExpress link with Gl45 stoppers.