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[cousinmuscles]

I did.
You did an AI analysis on a test made to test for the presence of only a single substance. The result was that it was probably moisture and solvents since nothing else turns up in the test.

Since the test does not test for anything else, obviously..nothing else would turn up?

English is my third language and I assume it must be your tenth, take that as a compliment for being multilingual
"There are no other hormones or anything else, the peaks are at the correct wavelengths, it seems they did a sloppy job in the purification and there are residual solvents, possibly moisture."

Where did I claim knowing what else is there vs assuming? Also, if it really was something ADDED after the manufacturing, wouldn't it be really hard to not notice when brewing or using the substance? Like the NPP that had floaters lol

 

[Photon]

"There are no other hormones or anything else,

There is nothing else, because the test was not designed to detect anything else. It does not prove if it exists or not.

If i dissolve poop in water, and test for water.
Water turns up.
Poop does not turn up in the test.
Can we say it does not exists?
No, because the test is not meant to test for poop.
Nothing else turns up, because we are only testing for water.

 

[Scooby69]

There is nothing else, because the test was not designed to detect anything else. It does not prove if it exists or not.

If i dissolve poop in water, and test for water.
Water turns up.
Poop does not turn up in the test.
Can we say it does not exists?
No, because the test is not meant to test for poop.
Nothing else turns up, because we are only testing for water.

For the 272nm chromatogram, there's a distinct smaller peak at approximately 1.5-1.6 minutes, eluting just before the main trestolone acetate peak at 1.850 minutes. It looks to be ~10-15% of main peak height at 272nm.

Hard to know what it is, but it could be free trestolone or it could be an oxidation product, but I would guess it is not a trestolone-related product since it is not showing up in the other wavelengths. I think some solvents would show up at this wavelength (272) and not the others. Of course, hard to know without further testing.

 

[cousinmuscles]

By the way, another member told me to check the weight, as they had gotten a little more as if they knew of the less purity. Turns out I got just over 11g, paid for 10.

 

[dinfaralt]

If i dissolve poop in water, and test for water.

ai say no poop g2g ill inject into my jugular vein

 

[cousinmuscles]

For the 272nm chromatogram, there's a distinct smaller peak at approximately 1.5-1.6 minutes, eluting just before the main trestolone acetate peak at 1.850 minutes. It looks to be ~10-15% of main peak height at 272nm.

Hard to know what it is, but it could be free trestolone or it could be an oxidation product, but I would guess it is not a trestolone-related product since it is not showing up in the other wavelengths. I think some solvents would show up at this wavelength (272) and not the others. Of course, hard to know without further testing.

Thanks, I asked ChatGPT about this and got this answer:

1. What you’re actually observing​

• Channel: PDA 272 nm
• Minor peak RT: ~1.5–1.6 min
• Main Trest peak RT: ~1.85 min
• Relative height at 272 nm: ~10–15% of the main peak

Key point:

This peak is NOT visible (or is negligible) at the primary quantitation wavelength (220–260 nm), but becomes visible at 272 nm.

That immediately tells us something important about its chemical nature.

---

2. Why “10–15% at 272 nm” ≠ “10–15% by mass”​

This is critical.

UV response is wavelength-dependent​

Different molecules absorb UV light very differently depending on:

• conjugation length
• double-bond placement
• oxidation state

A compound can:

• look small or invisible at 220–240 nm
• but look relatively large at 272 nm

Therefore:

A peak that is 10–15% of the main peak at 272 nm may correspond to 1–3% (or less) by mass.

This is why quantitation is done on Ch1 (220–260 nm), not at 272 nm.

---

3. What kind of compound shows up at 272 nm?​

272 nm is diagnostic. It strongly suggests a more conjugated chromophore than native trestolone acetate.

Likely candidates (ranked)​

A. Δ¹⁶ / Δ¹⁴ / extended enone isomer (MOST LIKELY)​

Trestolone synthesis commonly produces:

• double-bond–shifted isomers
• conjugation-extended enones

These:

• absorb weakly at 220–240 nm
• absorb strongly at ~270–280 nm
• elute slightly earlier (less hydrophobic)

This perfectly matches:

• RT 1.5–1.6 min (earlier)
• Visibility mainly at 272 nm

---

B. Mildly oxidized trestolone species​

Example:

• additional enone conjugation
• trace oxidation during workup or drying

These also:

• light up at 270+ nm
• stay UV-quiet at lower wavelengths

Likely contribution: very small.

---

C. Aromatized trace impurity (UNLIKELY but possible)​

True aromatization would:

• absorb strongly at 270–280 nm
• but would usually also show at 260–290 nm channels more clearly

Given the data:
This is less likely.

---

4. What it is almost certainly NOT​

Because of retention time and wavelength behavior, this peak is not:

• residual solvent
• moisture
• inorganic salts
• random contamination
• testosterone, nandrolone, etc.

Those either:

• don’t absorb UV
• or would show up clearly at 220–240 nm

---

5. How big is it​

Based on:

• absence from Ch1 quantitation
• appearance only at 272 nm
• relative elution behavior

A realistic estimate would be:

~1–3% structurally related trestolone isomer

That fits:

• the chemistry
• the UV physics
• the chromatographic behavior

---

6. How this fits with the earlier 11.9%​

This is important:

• The ~12% non-trest mass is still mostly solvent/moisture
• The 272 nm peak explains only a small fraction of that
• These two observations are not contradictory

Think of it as:

• ~85–90% trestolone acetate
• ~1–3% conjugated trest-related impurity
• ~7–10% solvent/moisture

That is very typical for high-end but non-pharma trestolone raw.

---

7. Is this a problem?​

Chemically:​

• No — this is a known, expected impurity class
• Common even in good syntheses
• Often removed only by additional recrystallization

Practically:​

• Biologically negligible
• Not toxic
• Not indicative of bad synthesis

Pharma material would spec this below 0.1%, but that requires:

• multiple recrystallizations
• aggressive drying
• much higher cost

---

8. How you’d confirm exactly what it is​

Definitive identification would require:

• LC–MS → molecular weight difference
• UV spectrum overlay → conjugation pattern
• Recrystallization + re-run HPLC → disappearance confirms isomer

---

Bottom line (precise and honest)​

The 1.5–1.6 min peak at 272 nm is almost certainly a minor, more-conjugated trestolone-related isomer, not 10–15% impurity by mass. Its true amount is likely low single-digit percent, and it does not explain the bulk of the non-trest mass, which remains dominated by solvent/moisture.

 

[cousinmuscles]

I'm not paying to figure out what that impurity is. For now since I did get circa 10% more than I paid for I am content. Won't try this until next year though.

 

[Photon]

I'm not paying to figure out what that impurity is.

I don't recommend it either
Not worth the $

 

[dinfaralt]

Thanks, I asked ChatGPT about this and got this answer:

stop asking chatgpt about stuff you cant interprept.

ive tried to use everything grok, gemini, chatgpt to try to research on this topic easier and its getting a brain fart from it.

 

[xrayphoton13]

stop asking chatgpt about stuff you cant interprept.

ive tried to use everything grok, gemini, chatgpt to try to research on this topic easier and its getting a brain fart from it.

Yeah i hate seeing the AI stuff. I skimmed through that whole thing and it told me nothing more than the previous poster that just said there was a shorter peak. The whole thing just reads like something a confident bullshitter would write

 

[BigTomJ]

2 liters of the stuff.

250mg
22bb
2ba
Mct oil

No issues, crystal clear

 

[cousinmuscles]

stop asking chatgpt about stuff you cant interprept.

ive tried to use everything grok, gemini, chatgpt to try to research on this topic easier and its getting a brain fart from it.

Yeah i hate seeing the AI stuff. I skimmed through that whole thing and it told me nothing more than the previous poster that just said there was a shorter peak. The whole thing just reads like something a confident bullshitter would write

I know lol, it will only use a limited amount of resources and check from a select database, but it does offer some insight and fact checking. You have to be aware of how short sighted it is and that it can't reason, thus give you wrong answers if you don't know for yourself what it all means.