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Thanks for your help. I might mess with it more and try other things to see if anything makes it better or worse.
Used a new vial - diluted to .5ml and no issues. Same bac water. Maybe that vial is off in some bit.Please share the results of whatever you discover through your experiments.
One other thing. Perhaps try .2um filtering the solution causing the reaction if you can and see if it's still doing it. I wonder if that vial developed aggregates for some reason and the other didn't. A tiny amount of silicon could cause that.
This might be a self explanatory answer here but on the theory of proper dilution ratios. Should you dilute the peptide in the 3ml vial it comes in and then transfer to a sterile let’s say 10ml vial and add the proper amount of bac water into that vial?
It spends the same time reconstituted if you reconstitute 1 mL into 10 vials or 10 mL into 1 vial. He's not dividing up the lyophilized powder into separate vials.