Help brewing L carnitine

[Walter Hype]

I make pipless L-carnitine 500mg/ml regularly, from bulk supplements.

264 ml distilled water ( heated /mag stirrer)
5ml HCI
Slowly add 250g L-carnitine bulk sups
( let stir for a few minutes will be a lil cloudy)
Add 5 ml BA
Add distilled water until you hit 500ml staring the entire time.
Filter through 22um filter (I use buchnar funnel with medium filter its actually a 20um) comes out crystal clear and ph is around 6-7

If you want to stick to something similar to the funnel idea but actually safe and sterile

Use a stainless steel clasp style

Buy 5 of them or more. They are great. Will never break. The filter is clasped using a special gasket and stainless steel plate. Zero bypass risk. Can be autoclaved. I use autoclave bags. Can put filter inside and just go to town. Can stack a pre filter on top if needed.

Accepts 47mm filter paper is readily available

PTFE is my preferred choice but I also use PES when I want water based.


Throughput is high and if your able to be quick and aspeptic you can hot swap filter paper but I tend to just swap funnels instead. Safer. Buy a few. They are very useful.


Your bottle top filter is fine however for water based. But if you do steroids I really recommend this. Many benefits going this route. Did I mention fully 316L stainless across the entire filter path?

OMICRON® FFSSN47 Stainless Steel 316 Membrane Filter Holder, 47mm Diameter, 300mL Capacity | LabX.com

OMICRON® FFSSN47 Stainless Steel 316 Membrane Filter Holder, 47mm Diameter, 300mL Capacity - New - $189.00 USD from Omicron Scientific Inc

www.labx.com

 

[JustJoe]

To make the L carnintine more isotonic I’ve read NaCL (sodium chloride) is very effective
Or you could use Glycine

My magnetic mixer comes this week and I’m going to give this recipe a try unless any of you have strong opinions about this recipe being bad.

-Lcarnintine base 25g
-Taurine 5g
-Sodium chloride .45g (help make it more isotonic)
-sodium citrate .10g (ph and buffer agent)
-citric acid .005g (ph and buffer agent)
-Benzyl alcohol .75ml
-sterile water 35ml initially just for displacement purposes then add remaining to get to 50ml

50ml multi use vial
500mg/ml L carnintine
100mg/ml taurine
Aiming for a ph level between 6.5 and 7

This will be my first time making L carnintine so we will see how it goes.

Sorry I forgot about this thread for a minute and might be way behind but adding NaCl with increase the tonicity even further. It increases the number of ionized molecules

At 500 mg/ml, L carn is already extremely hypertonic.


500 mg/ml is about 3.1 mol per liter, which works out to over 3000 mOsm per liter. Normal plasma is about 280 to 300 mOsm per liter. Tonicity depends on the number of dissolved particles, not their weight.

When sodium chloride is added, it dissociates into Na+ and Cl- ions, creating two osmotic particles per molecule. This means sodium chloride adds osmoles and does reduce tonicity.

Because tonicity is additive, the osmotic load from L carn remains and the nacl increases it further. The result is an even more hypertonic solution, which pulls water out of tissue

Even though people been doing it for years it’s outside the safety margins of any IM injectable. (I dilute mine)

 

[V.primal]

Sorry I forgot about this thread for a minute and might be way behind but adding NaCl with increase the tonicity even further. It increases the number of ionized molecules

At 500 mg/ml, L carn is already extremely hypertonic.


500 mg/ml is about 3.1 mol per liter, which works out to over 3000 mOsm per liter. Normal plasma is about 280 to 300 mOsm per liter. Tonicity depends on the number of dissolved particles, not their weight.

When sodium chloride is added, it dissociates into Na+ and Cl- ions, creating two osmotic particles per molecule. This means sodium chloride adds osmoles and does reduce tonicity.

Because tonicity is additive, the osmotic load from L carn remains and the nacl increases it further. The result is an even more hypertonic solution, which pulls water out of tissue

Even though people been doing it for years it’s outside the safety margins of any IM injectable. (I dilute mine)

How much do you dilute?

 

[JustJoe]

How much do you dilute?

I use 1g/3ml although I know scientifically that this is probably causing minor tissue damage.

 

[adictv]

When using distilled water is this boiled and cooled first before heating back up to add BA or is filtering at the end enough

 

[Dxt25]

When using distilled water is this boiled and cooled first before heating back up to add BA or is filtering at the end enough

Just use this

Biologix® Water for Injection(WFI), Sterile, 500mL/ Bottle, 10 Bottles/Case

WFI for cell culture is high-quality cell culture-grade water obtained by multiple distillations and sterilization using purified water. It meets the requirements for sterile water for injection in the Chinese Pharmacopoeia and is produced in an ISO-certified GMP factory.

biologixusa.com

 

[JustJoe]

When using distilled water is this boiled and cooled first before heating back up to add BA or is filtering at the end enough

Just use this

Biologix® Water for Injection(WFI), Sterile, 500mL/ Bottle, 10 Bottles/Case

WFI for cell culture is high-quality cell culture-grade water obtained by multiple distillations and sterilization using purified water. It meets the requirements for sterile water for injection in the Chinese Pharmacopoeia and is produced in an ISO-certified GMP factory.

biologixusa.com

If you google "dental supply" one of the top results is like an amazon marketplace for dental supply vendors, you can buy IV bags of sterile water for about $12 per liter, free 3 day shipping. They are what I use to make my Bac water, and brew. I store them with a rubber glove over the injection port, with the injection port up, to try and keep it aseptic as possible. you can buy lots of cool stuff there

If you are using distilled water, boiling it or microwaving it doesnt really do much: putting it in a proper container to boil adds more microbes than taking it straight out of a bottle

 

[Photon]

If you google "dental supply" one of the top results is like an amazon marketplace for dental supply vendors, you can buy IV bags of sterile water for about $12 per liter, free 3 day shipping. They are what I use to make my Bac water, and brew. I store them with a rubber glove over the injection port, with the injection port up, to try and keep it aseptic as possible. you can buy lots of cool stuff there

Some of these IV bags have DEHP and/or PVC. A lot of websites don't seem to list if that's the case, something to keep a look out for...

 

[sh1n]

Thanks for the Input brewed succesfully 50ml @500mg/ml 1,5%BA injection sub without pain

 

[ThreeSix]

Due to scar tissue buildup I decided to take as much stuff s.c. as I can. With l-carnitine, does it depend on the pH of the solution how long the injected fluid stays? I think I messed up the pH the last time and had injections stay there like forever, it feels like.

offtopic: what is your solution to scar tissue? At some sites at my leg, it's really hard to stick the needle in and even harder to push the fluid from the syringe...

 

[Photon]

Due to scar tissue buildup I decided to take as much stuff s.c. as I can. With l-carnitine, does it depend on the pH of the solution how long the injected fluid stays? I think I messed up the pH the last time and had injections stay there like forever, it feels like.

offtopic: what is your solution to scar tissue? At some sites at my leg, it's really hard to stick the needle in and even harder to push the fluid from the syringe...

The more concentrated the longer it takes to absorb, not so much the ph. You can try a massage gun.

 

[nudemember]

Is this what you guys are using as l-carnitine base? the ingredients listed shows just l-carnitine so i want to verify before i begin gathering supplies.

Could anyone provide a reasonably priced USP BA? I was going to grab it from medlabsupply but the shipping is more than the product lol. If that's the best source then so be it just curious if there's an alternative.

Are you guys sterilizing the powder in any way before brewing it or is filtering sufficient with properly sterilized equipment?

Thanks in advance and thanks to those who have provided quality information on this thread

 

[Photon]

Is this what you guys are using as l-carnitine base? the ingredients listed shows just l-carnitine so i want to verify before i begin gathering supplies.

Could anyone provide a reasonably priced USP BA? I was going to grab it from medlabsupply but the shipping is more than the product lol. If that's the best source then so be it just curious if there's an alternative.

Are you guys sterilizing the powder in any way before brewing it or is filtering sufficient with properly sterilized equipment?

Thanks in advance and thanks to those who have provided quality information on this thread

i prefer purebulk but yes this will work.
yes MLS ba.

you sterilize it when you brew finish and filter to vials.
why not get the 100ml ultra spec vials from MLS? they're USP rated at the very minimum.

 

[nudemember]

i prefer purebulk but yes this will work.
yes MLS ba.

you sterilize it when you brew finish and filter to vials.
why not get the 100ml ultra spec vials from MLS? they're USP rated at the very minimum.

I was looking at those vials but intended to order some with a few other things i need from gpzmedlab. Any input on these?

30 ML Sterile Amber Vial

Amber 30ML Presealed sterile vial - Type 1 borosilicate amber glass assembled with butyl stoppers and aluminum seals. Sterilization is performed with cGMP compliance in an FDA inspected facility. Assembled units are clean, sterile and meet the FDA's mandated 14-day sterility testing protocol...

www.gpzmedlab.com

 

[Photon]

I was looking at those vials but intended to order some with a few other things i need from gpzmedlab. Any input on these?

30 ML Sterile Amber Vial

Amber 30ML Presealed sterile vial - Type 1 borosilicate amber glass assembled with butyl stoppers and aluminum seals. Sterilization is performed with cGMP compliance in an FDA inspected facility. Assembled units are clean, sterile and meet the FDA's mandated 14-day sterility testing protocol...

www.gpzmedlab.com

no input
never bought them lol

i wouldn't use their vials for oils tho

 

[nudemember]

no input
never bought them lol

got ya, i'll grab the ultra spec then, thanks!

 

[PrairieSteel]

What a wicked thread. Just want to say I appreciate everyone contributing.

I think I'll brew my own l-carnitine now!