HGH Purity and dimer

[readalot]

That's fine. I'll red pen it today. Genuinely.

Where?

Did I miss it?

Or you didn't provide after Sampei provided a good plan?

 

[readalot]

Fyi...

https://thinksteroids.com/community...nt-state-of-generic-gh.134427828/post-3454976

Thesis with supplemental data:

https://thinksteroids.com/community...nt-state-of-generic-gh.134427828/post-3454989

 

[Elektrobot]

I'm new here and read through all 10 pages of this just to find 2 dead links at the end

I'm guessing the project never launched? What info was in the links posted above?

I learned a lot. I'd like to learn even more if this morphed into a different topic somewhere.

 

[AlexDavis43]

I'm new here and read through all 10 pages of this just to find 2 dead links at the end

I'm guessing the project never launched? What info was in the links posted above?

I learned a lot. I'd like to learn even more if this morphed into a different topic somewhere.

@Sampei is doing the experiment and @TheLobster is generously paying for the whole thing

 

[AlexDavis43]

FYI ChatGPT rots your brain

Don't let your kids use it

Stick with regular internet searches, no one is impressed by copied & pasted walls of AI text that can be spotted from a mile away

Link to the study in this article

Another article about the study (more user-friendly): here

These tips from a Forbes article if you need help quitting ChatGPT:

 

[Elektrobot]

@Sampei is doing the experiment and @TheLobster is generously paying for the whole thing

That is generous and we will be fortunate to have access to the resulting information.

 

[Elektrobot]

FYI ChatGPT rots your brain

Don't let your kids use it

Stick with regular internet searches, no one is impressed by copied & pasted walls of AI text that can be spotted from a mile away

Link to the study in this article

Another article about the study (more user-friendly): here

These tips from a Forbes article if you need help quitting ChatGPT:

View attachment 335084

Thats a good reminder. For me it's easy to forget how prevalent AI responses can be. I'm not used to AI existing yet- very little crossover between my work and daily life and AI so far. So I forget to watch for it in other places.

 

[AlexDavis43]

That is generous and we will be fortunate to have access to the resulting information.

Of course I read that in a monotonous robot voice xD

 

[Elektrobot]

Of course I read that in a monotonous robot voice xD

Haha, I started this username like 25 years ago and it's been what I use on all my random interest forums. I've always been a tinkerer and robotics hobbyist.

 

[Elektrobot]

Actually for per dose filtration. I draw the unfiltered reconstituted peptide into a 3ml syringe. then attach the filter and needle, and backfill an insulin syringe as needed, so each dose is "freshly filtered" just before administration, leaving no "incubation time" for new aggregate formation. So in other words, the "vial" of peptide is stored in the 3ml filtered syringe until it's used up.

I am intrigued by this method. It sounds great. I have a few points of curiosity

1. Do you "backwash" the filter after each dose so solution doesn't sit in it?

2. Do you purge the filter before each dose?

3. Do you think the filter could act as a matrix for undesired compounds to incubate in?

4. If we extended this methodology to oils, would they breakdown the plastics? Should oils be stored in glass only?

 

[Photon]

3ml with 18g needle:

BD Luer-Lok Syringe with PrecisionGlide Needle, Box of 100 (Click for available sizes)

BD Luer-Lok Syringe with PrecisionGlide Needle, 100 per Box BD Luer Lok Syringe Tip with PrecisionGlide Needle: Becton Dickinson and Company: PrecisionGlide Hypodermic Syringe with needle combination. World's sharpest needle is combined with BD's clea

www.medical-and-lab-supplies.com

Most of us here are using Nipro needles.
Precision-glide comes at a significant premium.
Have you tried both and are you able to compare them?

Nipro Sterile Hypodermic Needles, Box of 100

FDA Approved Nipro Sterile Hypodermic Needle comes in Box 100. This Ultra-sharp, three-beveled siliconized needle use to minimize patient discomfort during injection. It's Compatible with both luer slip and luer lock syringes.

www.medical-and-lab-supplies.com

 

[Ghoul]

I am intrigued by this method. It sounds great. I have a few points of curiosity

1. Do you "backwash" the filter after each dose so solution doesn't sit in it?

2. Do you purge the filter before each dose?

3. Do you think the filter could act as a matrix for undesired compounds to incubate in?

4. If we extended this methodology to oils, would they breakdown the plastics? Should oils be stored in glass only?

1. No. The last drop falls or is shaken off the needle tip. That leaves a continuous liquid path from slightly up the needle lumen all that back to the syringe. Where’s bacteria going to enter? Up the needle? It’s not as if I set the needle down on a surface without the cap on. I do swipe the needle with an alcohol swab before filling the insulin syringe and when finished before putting the cap back on.

2. No. There are no extractables, or any other interactions, between PES filter media or the polypropylene housing and 0.9% Benzyl Alcohol. An interesting note is that for a syringe filter to qualify for human medical use, it has to pass a “grow through” test where bacterial is grown on one side of the filter, and it has to be verified none “grows through” to the other side. As a practical matter, any modern PES filter needs to meet this standard to function properly for lab use, although I use FDA pharma grade filters (because I thought I had to when I bought them).

3. Not on the sterile side of the filter path. And no more on the “unsterile” side than would occur in the vial.

4. There’s no point with oils. With proteins, this process ensures any newly formed aggregates are removed just before administration. This is the only advantage vs filtering the entire vial at once.

I’m essentially putting a process called “bedside filtration” into practice, something the leading developers of protein drugs have been proposing for the last few years:



Here’s data showing a reduction in particles in 1ml of rHGH >1um in size from 36,000 to 300 after filtering.


This is the tested level of immunogenicity response the FDA found in commercial GLP Injectable drugs, on the left of each chart, and the 2 GLPs on the right are from the closest we have to UGL, small compounding pharmacy GLPs. See how much worse it is with the compounded peptides?



This is immunogenicity of the same compounds before and after filtering. Look at the compounded pharmacy example on the right. Immunogenicity was reduced by 80%. There’s really no explanation for that other than the removal of protein aggregates.

 

[Elektrobot]

1. No. The last drop falls or is shaken off the needle tip. That leaves a continuous liquid path from slightly up the needle lumen all that back to the syringe. Where’s bacteria going to enter? Up the needle? It’s not as if I set the needle down on a surface without the cap on. I do swipe the needle with an alcohol swab before filling the insulin syringe and when finished before putting the cap back on.

2. No. There are no extractables, or any other interactions, between PES filter media or the polypropylene housing and 0.9% Benzyl Alcohol. An interesting note is that for a syringe filter to qualify for human medical use, it has to pass a “grow through” test where bacterial is grown on one side of the filter, and it has to be verified none “grows through” to the other side. As a practical matter, any modern PES filter needs to meet this standard to function properly for lab use, although I use FDA pharma grade filters (because I thought I had to when I bought them).

3. Not on the sterile side of the filter path. And no more on the “unsterile” side than would occur in the vial.

4. There’s no point with oils. With proteins, this process ensures any newly formed aggregates are removed just before administration. This is the only advantage vs filtering the entire vial at once.

I’m essentially putting a process called “bedside filtration” into practice, something the leading developers of protein drugs have been proposing for the last few years:

View attachment 370629View attachment 370630

Here’s data showing a reduction in particles in 1ml of rHGH >1um in size from 36,000 to 300 after filtering.
View attachment 370631

This is the tested level of immunogenicity response the FDA found in commercial GLP Injectable drugs, on the left of each chart, and the 2 GLPs on the right are from the closest we have to UGL, small compounding pharmacy GLPs. See how much worse it is with the compounded peptides?

View attachment 370632

This is immunogenicity of the same compounds before and after filtering. Look at the compounded pharmacy example on the right. Immunogenicity was reduced by 80%. There’s really no explanation for that other than the removal of protein aggregates.

View attachment 370633

Sounds like a solid improvement plan for the new year!

I saw the mention of syringe and needle lubricants. I just reupped all my stock without any consideration for that :/

 

[Ghoul]

Sounds like a solid improvement plan for the new year!

I saw the mention of syringe and needle lubricants. I just reupped all my stock without any consideration for that :/

It’s preferable to go silicone free, but filtering will remove some of the silicone oil drops and the main target, aggregates.

Haven’t tried these personally, and you have to go to 5ml (no 3ml). But for $20 / 100 hard to beat for Silicone Free syringes.

Syringes – UltraCruz® | SCBT - Santa Cruz Biotechnology

UltraCruz® Syringes feature Luer Lock or Luer Slip system & are ideal for a variety of inoculation & sampling tasks in lab or veterinary applications

www.scbt.com

 

[Elektrobot]

It’s preferable to go silicone free, but filtering will remove some of the silicone oil drops and the main target, aggregates.

Haven’t tried these personally, and you have to go to 5ml (no 3ml). But for $20 / 100 hard to beat for Silicone Free syringes.

Syringes – UltraCruz® | SCBT - Santa Cruz Biotechnology

UltraCruz® Syringes feature Luer Lock or Luer Slip system & are ideal for a variety of inoculation & sampling tasks in lab or veterinary applications

www.scbt.com

Those look great! Thank you

I was fixated on finding luer lock syringes that have a draw needle pre-attached so I can live a life of luxury when getting my shots ready. Totally overlooked the more important consideration of lubricants.

I'm going to grab those in 1ml and 10ml. I usually recon my 36iu hgh with 7.2ml bac

*** awe fck. The 1ml are luer slip. Time to start filling 2 mil syringes

 

[Photon]

It’s preferable to go silicone free, but filtering will remove some of the silicone oil drops and the main target, aggregates.

Haven’t tried these personally, and you have to go to 5ml (no 3ml). But for $20 / 100 hard to beat for Silicone Free syringes.

Syringes – UltraCruz® | SCBT - Santa Cruz Biotechnology

UltraCruz® Syringes feature Luer Lock or Luer Slip system & are ideal for a variety of inoculation & sampling tasks in lab or veterinary applications

www.scbt.com

Interesting to see these.
They are reselling the air tite syringes.
The 2ml fits 3ml.
You can get them on a few websites.

I have them all the way to 60ml.
It's solvent resistant and I use them for brewing.

They don't push as easily as the normal syringes, but that's to be expected without the lubricant.

Air-Tite Products Co., Inc. - 2-Part Luer Lock Syringes

2-Part Luer Lock Syringes

www.air-tite-shop.com

 

[shcidheuddvv]

Better deals elsewhere:

Buy Ultra Spec Sterile 2ml Clear Sealed Glass Vials at wholesale prices

In order to receive vials in sealed shrink wrap boxes, please order in multiples of 25

www.medical-and-lab-supplies.com

3ml with 18g needle:

BD Luer-Lok Syringe with PrecisionGlide Needle, Box of 100 (Click for available sizes)

BD Luer-Lok Syringe with PrecisionGlide Needle, 100 per Box BD Luer Lok Syringe Tip with PrecisionGlide Needle: Becton Dickinson and Company: PrecisionGlide Hypodermic Syringe with needle combination. World's sharpest needle is combined with BD's clea

www.medical-and-lab-supplies.com

or get syringes without needle and use these, the ideal peptide reconstitution needles:

BD Nokor Admix Non-Coring Needles, 18G x 1.50", Box of 100

BD Nokor Admix Non-Coring Needles, 18G x 1.50" (38mm) Part No.: 305215 Features: Vented needles* help equalize pressure to reduce blowback during medication preparation from a vial. BD Nokor™ Needles feature a cannula side port to place fluid on the side wall of the container during...

www.medical-and-lab-supplies.com

Filters are excessively priced, these are a much better deal. 13mm, .22um (no prefilter).

Sterile 0.2 μm PES Syringe Filters

Hydrophilic PES syringe filters with single or double-layer membrane. Pore sizes of 0.2μm. Diameter of 13mm/25mm/33mm with Luer lock. Sterile individually packed.

shop.cobetter.com

Hey Ghoul, may be a dumb question, can I store filtered oils, specifically Zydus Testoviron in Arachis oil, in these Ultra Spec Sterile vials? I didn't know if the rubber stoppers have to be special in order to not degrade or contaminate the oil.

 

[Ghoul]

Hey Ghoul, may be a dumb question, can I store filtered oils, specifically Zydus Testoviron in Arachis oil, in these Ultra Spec Sterile vials? I didn't know if the rubber stoppers have to be special in order to not degrade or contaminate the oil.

Yes, ultra spec vials use teflon coated stoppers, which blocks interactions between the rubber and oil/solvents. So none of the nasty chemicals in butyl rubber leech into what you’re injecting.

 

[setters]

Curious if anyone has experienced an actual difference in an unfiltered versus filtered vial of GH. I've never filtered and have used several Chinese generics. The idea that they would cause worse classic GH side effects (water retention, joint pain) does not make any sense, the biggest risk would seem to be an ISR or worse bacterial infection, which you'd know about right away.