Infection FML

[Info.only]

Hey guys,


So I have brewed a few times now with success, using Bill R's recipe (no BA).

Now, however, I've found my current batch almost certainly has a bacterial contaminant, and have mild cellulitis at two injection sites.

Curious if anyone has ideas as to what the issue may have been.


More info:

This batch is TP, using syringe filtration.

Used BB at 9%, no BA. .45um nalgene sterile filter.

To my knowledge, no membrane ruptures.

Oil was expressed grape seed oil, but not USP.

No hear used.

Injected into sterilized 10ml vial bought from Med Lab.


Thanks for any input!


Also, I just assume I should destroy the entire yield. If anyone disagrees and thinks I should hear treat and the. Add BA, I'm open to thoughts.

 

[Astro Labs]

No BA? Well theres your reason! Why in the world would you not sterilize the oil your injecting? A FILTER WILL REMOVE BACTERIA BA ASSURES NO BACTERIA WILL FORM for a reasonable amount of time. This is the reason that some forums exclude the talk of homebrew. I'm all for members saving money but if you are clueless leave it alone an infection can cost you your life. Maybe the odds are in your favor but why risk it for literally a few bucks?

 

[pumpingiron22]

[emoji85]

 

[Oregongearhead]

No BA? Well theres your reason! Why in the world would you not sterilize the oil your injecting? A FILTER WILL REMOVE BACTERIA BA ASSURES NO BACTERIA WILL FORM for a reasonable amount of time. This is the reason that some forums exclude the talk of homebrew. I'm all for members saving money but if you are clueless leave it alone an infection can cost you your life. Maybe the odds are in your favor but why risk it for literally a few bucks?

No alcohol in the gear ? Astro came out of his element to talk on this one , you know you screwed-up ! ~Ogh

 

[GigaloRob]

It's like banging a 20 year veteran hooker with no condom and then wondering why you have herpes

 

[Info.only]

All the burns were well deserved guys.

And yeah, Astro, it's actually awesome to be burned by a lab

No sarcasm, I earned this infection and the criticism by being ill-prepared.

That veteran hooker gave my the cellulitis.

Going to order from someone who knows what the hell they're doing for now.

Thanks fellas!

 

[MANWHORE]

A .45 Filter will not Filter out ALL bacteria..
A Staff Spore can get through a .45..

Even using a .22, I still use BA

 

[nophat]

Wtf where you thinking!!!
GO BACK AND READ AGAIN
Then again stop !!

 

[Oregongearhead]

All the burns were well deserved guys.

And yeah, Astro, it's actually awesome to be burned by a lab

No sarcasm, I earned this infection and the criticism by being ill-prepared.

That veteran hooker gave my the cellulitis.

Going to order from someone who knows what the hell they're doing for now.

Thanks fellas!

Kudo's to Info for taking it well and not having a rookie meltdown . He's learned his lesson and won't be making that mistake again ......congrats ~Ogh

 

[nolimitz]

I've never heard of people recommending no BA use or filtering through a .45um only. It's pretty common knowledge among all homebrewers to use a .22um and BA. The BA prevents bacteria from forming. Think about it, every time you draw from the vial, you are contaminating it. The needle is contaminated once you remove it from the sterile wrapper.

 

[calfcramp]

You have to use ba

 

[Info.only]

Thanks again to everyone. And again, I entirely deserve any flack.

I'll be taking this as a hard-earned lesson to never listen to just one "expert" or to dive into something without literally knowin the very basics first.

To not know that BA was needed seems comprable to not knowing knowing that prop is an injectable, except for the fact no one has contracted a infection from drinking test

My embarrassment is no less than I earned.

 

[Info.only]

I've never heard of people recommending no BA use or filtering through a .45um only. It's pretty common knowledge among all homebrewers to use a .22um and BA. The BA prevents bacteria from forming. Think about it, every time you draw from the vial, you are contaminating it. The needle is contaminated once you remove it from the sterile wrapper.

Yes, what you and manwhore said was (of course) entirely on point. If you fail to filter ALL bacteria from the sample, it is only aseptic, and so the few that are left will from into colonies.

While a .45um filter is used to test samples for sterility, it is well known (not by me ) that this is only relative, in that it is assumed any contaminated sample will have colonies large enough for detection with this size of filter. That does not mean a .45um, or even a .22um filter will eliminate all bacteria. Thus the need for a bacteriostatic agent that will inhibit the remaining bacteria from growing.

See what twenty minutes of reading could have saved me? See what a handful of posts from meso members could have helped me avoid? Sh&t



Still not going to go anywhere near home brewing for a long time.

 

[chuckman]

You should check out Basskiller website chick on steroids and he has a step by step how to do it yourself be safe and good luck.

 

[Info.only]

Yeah checked out basskiller. Really enjoyed the site!

 

[Kubrick]

You have to use ba

And bubble wrap if you're mailing it!

 

[weighted chinup]

And bubble wrap if you're mailing it!

 

[kawilt]

Idi probably just had lunch!!

 

[weighted chinup]

LIke your avatar.....he probably just had lunch!!

Thanks! And yeah that gif always make me smile