Janoshik Analytical laboratory testing services

[readalot]

Response from Janoshik:

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1. Yes, sure.

We are following the USP endotoxin testing SOP (Bacterial Endotoxins | USP) using a quantitative Pierce™ Chromogenic Endotoxin Quant Kit (Pierce™ Chromogenic Endotoxin Quant Kit). Positive and negative controls are further described in the attached documents, and are included with every analyzed batch of samples along with a 5-point calibration curve in triplicate (same as samples).

2. There will be no extra charge from our side of things.

Thank you.


With best regards,
Kate
Assistant - Janoshik Analytical

Janoshik Analytical

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Fyi for those that had questions on the endotoxin assay SOP and calibration.

@Ghoul
@MyNameIsJeff

Also, a very generous offer from you @janoshik to cost share on the HPLC runs if we can get you the standard. I'd also be willing to fund raise for GCMS analysis of the standard if you think that is double check.

So there you have it folks. Opportunity for the community here.

I've had two persons offer to pitch in thus far...

Thanks very much @keenpen and @bigMoJo.

Has the LAL test protocol been requested? This is something the lab should be willing to supply to any customer, it's a standard expectation with the service.

I ask because I'm getting a little irritated with the "free from endotoxins" and "proves there's no need for testing" comments.

To be frank, I'm skeptical of results that are cleaner than what we'd typically see from a compounding pharma making Testosterone oils using a completely aseptic process in an FDA approved facility by techs wearing bunny suits.

??

Lost interest on your endotoxin questions?

https://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/highly-sensitive-assay-endotoxin-detection-quantitation-variety-sample-types-white-paper.pdf

 

[RandallFlagg]

I'm late to this...but happy to pitch in. Just need details on amount and how to send funds. DM should work but post back here if not.

 

[readalot]

I'm late to this...but happy to pitch in. Just need details on amount and how to send funds. DM should work but post back here if not.

Thanks for your offer to participate. We are up to 9 and will pick up in the New Year. Will be great question to answer. Merry Christmas Brother!

 

[Trenbolonetax]

Make it 10. If XMR donations are accepted.

 

[narta]

XMR

 

[readalot]

 

[readalot]

Make it 10. If XMR donations are accepted.

Thanks Bro. We will figure it out in the new year. Gotta figure out a spot to get all the details worked out where there is no surveillance. Be careful.

I think I got a tail.

 

[RandallFlagg]

Thanks for your offer to participate. We are up to 9 and will pick up in the New Year. Will be great question to answer. Merry Christmas Brother!

Merry Christmas!

Kudos to you for keeping your head down and pushing on. I will confess I lack your persistence in this pursuit but am supportive of the measured way you have gone about implementing a vision.

(Yes, I know there are plenty of detractors and they're entitled to their opinions..though the ones who just showed up in the last 6 months and have no prior tainted history with you really should just fuck right off...) Others who have been around a while...well, I can't speak to whatever Readalot 1.0 was like, so it's possible it was hard to forget..lol.

May 2025 bring us all a shared vision of harm reduction idea-sharing and productive sparring...(Even if no packs get into the USA...)

 

[readalot]

I can't speak to whatever Readalot 1.0 was like

Same PITA after I got attacked for simply bringing up the concept.

Thread 'Source QC and C of A (do you have one?)'

Oct 19, 2023

Some thoughts/suggestions for discussion after looking around here.

Realistic, overkill, get what you pay for, don't care?

Would like to hear members' thoughts, counterpoints, etc. especially on cost vs quality. The path to quality is known. Will people pay or too much for UGL customers?

=======

Every Source should provide with each shipment ...

1. 3rd party C of A on finished product batch specific to that shipment
- endotoxin screen
- metals with ICP
- purity(ies) / concentration(s) using hplc plus ms (or gcms)

2. C of A for bulk api for each batch (hplc plus ms or gcms)...

• readalot
• analytical harm reduction manufacturer source testing
• Replies: 333
• Forum: Steroid Underground

• 
• 

Lit a fire. I started very civil.

 

[Ghoul]

??

Lost interest on your endotoxin questions?

https://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/highly-sensitive-assay-endotoxin-detection-quantitation-variety-sample-types-white-paper.pdf

Digesting this. Obviously I'm no expert, but I know that lipid based compounds, vs aqueous solutions, are challenging because the lipids coating molecules interfere with the LAL reaction. I understand this can be overcome with special techniques. In the process I looked at; a custom reference standard had to be created for this lipid endotoxin test to validate everything.

I've been looking for info regarding a lipid specific process using the kit they test with but haven't come up with anything so far.

 

[RandallFlagg]

Same PITA after I got attacked for simply bringing up the concept.

Thread 'Source QC and C of A (do you have one?)'

Oct 19, 2023

Some thoughts/suggestions for discussion after looking around here.

Realistic, overkill, get what you pay for, don't care?

Would like to hear members' thoughts, counterpoints, etc. especially on cost vs quality. The path to quality is known. Will people pay or too much for UGL customers?

=======

Every Source should provide with each shipment ...

1. 3rd party C of A on finished product batch specific to that shipment
- endotoxin screen
- metals with ICP
- purity(ies) / concentration(s) using hplc plus ms (or gcms)

2. C of A for bulk api for each batch (hplc plus ms or gcms)...

• readalot
• analytical harm reduction manufacturer source testing
• Replies: 333
• Forum: Steroid Underground

• 
• 

Lit a fire. I started very civil.

 

[readalot]

Digesting this. Obviously I'm no expert, but I know that lipid based compounds, vs aqueous solutions, are challenging because the lipids coating molecules interfere with the LAL reaction. I understand this can be overcome with special techniques. In the process I looked at; a custom reference standard had to be created for this lipid endotoxin test to validate everything.

I've been looking for info regarding a lipid specific process using the kit they test with but haven't come up with anything so far.

Thanks for the reply. Merry Christmas and I'll see if I can dig up the variance on oils testing or if the lab Jano uses such a technique. Good points.

https://www.americanpharmaceuticalreview.com/Featured-Articles/559025-Bacterial-Endotoxins-Testing-in-Lipid-Based-Drug-Formulations-Using-Liquid-Liquid-Extraction/

 

[godsgifttoearth]

@janoshik what pricing does minoxdil tabs fall under for single and 3 tabs?

 

[Ghoul]

Thanks for the reply. Merry Christmas and I'll see if I can dig up the variance on oils testing or if the lab Jano uses such a technique. Good points.

https://www.americanpharmaceuticalreview.com/Featured-Articles/559025-Bacterial-Endotoxins-Testing-in-Lipid-Based-Drug-Formulations-Using-Liquid-Liquid-Extraction/

Yea, that article is precisely what brought all the complications in testing lipid based parenterals to my attention.

That and the fact results so far seem to indicate UGLs are doing an even better job than billion dollar pharma plants at keeping endotoxin to effectively undetectable levels, a remarkable achievement considering they're using unsterile ingredients, not working in aseptic environments, and can't keep floaters out of vials consistently.

 

[iris]

Yea, that article is precisely what brought all the complications in testing lipid based parenterals to my attention.

I haven't followed this lately, but is this about the test that is being currently offered not being accurate/reliable and needing change/improvement of standards?

 

[Ghoul]

I haven't followed this lately, but is this about the test that is being currently offered not being accurate/reliable and needing change/improvement of standards?

I'm not in a position to say if it's accurate or not, but testing oils for endotoxin is difficult and out of the ordinary enough for the premier pharmaceutical journal to publish a long paper on how a team overcame those obstacles, and if nothing else, the process needs to be reproducible to get consistent results, so we need to understand precisely how it's being done.

 

[janoshik]

@janoshik what pricing does minoxdil tabs fall under for single and 3 tabs?

Quote number #18829 :
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1x Minoxidil analysis x 240 USD = 240 USD
2x Additional pill tested x 40 USD = 80 USD
-------------------------
TOTAL: 320 USD

This offer remains valid until January 10, 2025.

Cheers

I'm not in a position to say if it's accurate or not, but testing oils for endotoxin is difficult and out of the ordinary enough for the premier pharmaceutical journal to publish a long paper on how a team overcame those obstacles, and if nothing else, the process needs to be reproducible to get consistent results, so we need to understand precisely how it's being done.

Right now we're in the process of moving endotoxin analyses in-house, so we'll have the process under much better control.

Should be done in roughly 30 days.