MALDI-TOF-MS/HPLC-UV-VIS rHGH results

Wow. I think you are just fucking around now, aren't you? I think you are.

I don't care if your link leads to Karl's GH, Eli Lilly's, the FDA, the Center for Disease Control and Prevention, or the Holiday Inn. That link is a MASS SPEC, for fuck sake!!! It is NOT an HPLC. So it couldn't possibly have been used for the HPLC standard! Please, in the name of all that is holy, don't reply again until that sinks in to that thick head of yours.

This has way gone beyond silly. If we're just going to fuck around in this thread, let me know. I can post nonsense all night long.

Let me elaborate one more time. The standard used in the equation for calulating the concentrations is AS1625-2. In all the lab reports ever posted by Jim, I only see 1 AS1625-2, and that is for Karl's 20iu Somastim. Since you are in the know, please correct me with the accurate information where the standard AS1625-2 was established. Its the information I have been requesting for a long time and it keeps being dodged. I think its evident at this point why its being dodged.
 
I'm not pissed... I'm tired of hearing this bullshit.. No one is even listening to you anymore... You look freaking ridiculous.

Come out of your little Meso world and into the real world and lets see what people actually think. I am not here for a meso popularity contest. I am here to get things right. If you think I have an agenda or are here for any other reason, then its because you are ignorant of what goes on outside of meso.
 
Let me elaborate one more time. The standard used in the equation for calulating the concentrations is AS1625-2. In all the lab reports ever posted by Jim, I only see 1 AS1625-2, and that is for Karl's 20iu Somastim. Since you are in the know, please correct me with the accurate information where the standard AS1625-2 was established. Its the information I have been requesting for a long time and it keeps being dodged. I think its evident at this point why its being dodged.

https://thinksteroids.com/community/attachments/as1776-1-4-w-std-summary-070215-pdf.26903/
 
Now I'm selling Pharm grade GH LMFAO. Bozo I cant even use the stuff bc of crippling CTS symptoms and I suspect MANDS is aware of this bc I've mentioned it to him and have posted it on this forum several times and LONG BEFORE, I tested anything. (SUCKS TO BE WRONG DOESN'T IT) Moreover you may believe others are willing to risk their livelihood bc you haven't one, but I'm not!

I't seems your running out of straws Meat Head

Don't fret bc I recently heard the data sets are all wrong and the results should be multiplied BY FOUR, (is that why mates "needed ten IU" LOL) this coming from a "chemist" according to a PM poster. Well let's put it to you this way that "chemist" doesn't know shit from Shinola either bc all this is taken into account when the standard concentration is entered into the HPLC computer.

WHAT was entered into the computer is listed at the TOP of each HPLC and along side of the samples alpha numerical assignment for instance AS1776-1 (10uL plus 40uL-10uLinj data) !!!!!!
Why is this done, to ensure the AUG units are the same as the standard being used, in this instance mg/ml!

Now just run along MH and embrace your little duckling PM members before you make a total fool out of yourself. Quack Quack !
 
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So then Jim, when the report refers to the standard AS1625-2, what GH is this. Is this Humatrope as you stated earlier or is it Karl's GH which is label as AS1625-2 in the mass spec? Are there 2 AS1625-2's, how can that be? Why can't we get simple answers? Why does everything have to be a knockout dragdown fight? Why so much evasion if everything is on the up and up?
 
Just so we are all clear, the question that I keep asking and has yet to be answered straight out is:

What GH is AS1625-2, The link CBS posted has names to all the GH's except the standard that was used. What brand GH is it and how was it originally supplied for testing? If you are disputing that AS1625-2 is Somastim supplied in a 20iu vial as labeled in the mass spec; then please show where this second AS1625-2 was ORIGINALLY tested and confirmed to be legit as I cannot find a lab report anywhere for it.

Please do not deflect or say anything other than the pertinent information for the questions posted. If you continue to dodge these questions it will be obvious as to the reason why.
 
I can guarantee you that @Dr JIM does NOT sell, profit or gain from any Pharm grade GH. I would bet my life on it.

As far as the sample used for standard with the original tests @CensoredBoardsSuck you can't tell me you aren't following @muscle96ss post and his concerns about the standard AS1625-2 being used as THE Standard. That number is associated with Karl's GH. I sent that sample in initially some time ago and Dr JIM stated that Karl was re-bottling Pharm Grade GH.

All of this that's going on here was supposed to educate MESO and the community. It's only making us here at MESO look like a cluster fuck.

By the way CBS this is my home board and always will be. I've was here way before you came around and I will be here way after you leave. You say I'm so excited to become in some inner circle at PM. I don't try and conform to any inner circles. I'm respected and excepted to many boards because I actually contribute and look out for the community.

mands
 
As far as the sample used for standard with the original tests @CensoredBoardsSuck you can't tell me you aren't following @muscle96ss post and his concerns about the standard AS1625-2 being used as THE Standard. That number is associated with Karl's GH. I sent that sample in initially some time ago and Dr JIM stated that Karl was re-bottling Pharm Grade GH.

The only thing I'm following is the only test result Muscles ever would have accepted is one that gave him the result he wanted. When his objections to the HLC have been answered, he finds something else (and equally ridiculous) to complain about. It is obvious that he will never accept the HPLC.

If you have concerns about the HPLC, why don't you come out and say it? This is your testing thread. You were involved with the testing process from the beginning to the end and you know how it was done, what standards were used, etc., and, more importantly, you know the results are accurate. And yet you have remained completely silent while Muscles tries to destroy its credibility.


All of this that's going on here was supposed to educate MESO and the community. It's only making us here at MESO look like a cluster fuck.

No, it's making those who reject the HPLC look like they have an agenda because their objections are silly and frivolous.

By the way CBS this is my home board and always will be. I've was here way before you came around and I will be here way after you leave. You say I'm so excited to become in some inner circle at PM. I don't try and conform to any inner circles. I'm respected and excepted to many boards because I actually contribute and look out for the community.

And I don't?
 
The only thing I'm following is the only test result Muscles ever would have accepted is one that gave him the result he wanted. When his objections to the HLC have been answered, he finds something else (and equally ridiculous) to complain about. It is obvious that he will never accept the HPLC.

If you have concerns about the HPLC, why don't you come out and say it? This is your testing thread. You were involved with the testing process from the beginning to the end and you know how it was done, what standards were used, etc., and, more importantly, you know the results are accurate. And yet you have remained completely silent while Muscles tries to destroy its credibility.




No, it's making those who reject the HPLC look like they have an agenda because their objections are silly and frivolous.



And I don't?
I have expressed my concerns to Jim about the concentration. He explained it and I'm still up in there air. To me it doesn't make sense why the biological assays DO NOT jive with the lower concentration data.

I'm not saying they are wrong but rather what is causing the good IGF-1 scores in the "generic" gh if the concentration is low.

mands
 
As far as the sample used for standard with the original tests @CensoredBoardsSuck you can't tell me you aren't following @muscle96ss post and his concerns about the standard AS1625-2 being used as THE Standard. That number is associated with Karl's GH. I sent that sample in initially some time ago and Dr JIM stated that Karl was re-bottling Pharm Grade GH.

The AS1625-2 linked by Muscles is for a MASS SPEC, not an HPLC. Whether or not the chemist uses similar identifiers for mass specs and HPLCs, or whether or not it was a typo, I have no idea, but in this instance, it's clear that AS1625 is referring to the HPLC's Humatrope standard.

The standard's (AS1625) 3.72 mg/ml concentration and 65,493,455 absorbance data were reported and you know it. You provided the link. Muscles is pretending he can't see it. Why are you giving credibility to Muscles' ridiculous objections instead of correcting his misinformation?

Untitled11.jpg
 
I can guarantee you that @Dr JIM does NOT sell, profit or gain from any Pharm grade GH. I would bet my life on it.

As far as the sample used for standard with the original tests @CensoredBoardsSuck you can't tell me you aren't following @muscle96ss post and his concerns about the standard AS1625-2 being used as THE Standard. That number is associated with Karl's GH. I sent that sample in initially some time ago and Dr JIM stated that Karl was re-bottling Pharm Grade GH.

All of this that's going on here was supposed to educate MESO and the community. It's only making us here at MESO look like a cluster fuck.

By the way CBS this is my home board and always will be. I've was here way before you came around and I will be here way after you leave. You say I'm so excited to become in some inner circle at PM. I don't try and conform to any inner circles. I'm respected and excepted to many boards because I actually contribute and look out for the community.

mands
Thank you Mands. Your first hand account is necessary because nobody at meso will believe anything else that counters CBS or Jim on this it seems. You have credibility here at Meso. I'm a fan of the board and those guys. They are generally dead on. But something is just off about this entire thing and the way Jim has handled it is strange. It's like he can't possibly admit there could have been a minor oversight when you yourself even raise some doubt. Things don't seem to add up for several reasons. The most SIMPLE being, who goes through the expense and trouble to produce or purchase almost pharm grade level gh only to GROSSLY underdose each vial (all brands, all samples). It's easily detectable and would result in shitty gh serum and IGF-1 results which is how these guys "advertise" now. This would just be a silly approach. Second, all the GH serum and IGF testing done seems to counter these massively underdosed result samples.

I have no idea what really happened but I certainly think something is off and Jim's handling of reasonable questions has been disappointing to say the least.

I don't really have any doubt about the monetary interests of detractors here (or PM's generic gh gatekeeper there), yes it's clear. That's still not the issue here. The questions raised are nonetheless valid. Just doesn't seem to add up. If the solution (equation used and data breakdown, sample/standard identification, etc) is so clear and simple, and Jim is so wise, why has he not broken it down and explained the alleged discrepancy for the laymen here. Why haven't you helped explain CBS? You're clearly more ahead on your understanding of that testing than most here, or at least portray that. All I see is you try and discredit Muscle96ss. Such an explanation would irrefutably crush the argument and detractors if it were done convincingly. Not sure why we haven't seen that yet.
 
The AS1625-2 linked by Muscles is for a MASS SPEC, not an HPLC. Whether or not the chemist uses similar identifiers for mass specs and HPLCs, or whether or not it was a typo, I have no idea, but in this instance, it's clear that AS1625 is referring to the HPLC's Humatrope standard.

The standard's (AS1625) 3.72 mg/ml concentration and 65,493,455 absorbance data were reported and you know it. You provided the link. Muscles is pretending he can't see it. Why are you giving credibility to Muscles' ridiculous objections instead of correcting his misinformation?

Untitled11.jpg
Jesus CBS!!! Look at the sample associated with AS1625. It's Karl's GH.

https://thinksteroids.com/community/attachments/img001-pdf.16049/

What am I missing?

mands
 
The only thing I'm following is the only test result Muscles ever would have accepted is one that gave him the result he wanted. When his objections to the HLC have been answered, he finds something else (and equally ridiculous) to complain about. It is obvious that he will never accept the HPLC.

If you have concerns about the HPLC, why don't you come out and say it? This is your testing thread. You were involved with the testing process from the beginning to the end and you know how it was done, what standards were used, etc., and, more importantly, you know the results are accurate. And yet you have remained completely silent while Muscles tries to destroy its credibility.




No, it's making those who reject the HPLC look like they have an agenda because their objections are silly and frivolous.



And I don't?

CBS, I personally use the grey top GH and recommend it to people who choose generic GH because it is the most consistent GH on the market based on both serum GH's and IGF-1's(as well as Karl's is up there too). In a UG market full of scammers and thiefs, there are only a few sources and a few generic GH's that have stood the test of time, so my agenda is to keep people from throwing their money away on trash. My pocketbook is unaffected by what GH anybody purchases, it always has and it always will. Anybody who knows my reputation knows that I am a straight shooter and my goal is to guide people in the right direction. I would never tell someone to use a product that I don't use and believe in myself.

In addition, you are probably correct in that I will never accept that the greys contain 4iu. The reason is simple. I have been using GH for many decades and have used a ton of both pharm and generic GH. I know the difference between good and bad GH and I get labs at different times to back that up. The sample you tested came directly from a kit that I have personally used and from a set of kits that several of my friends have used. So, I know for a fact that there is no way that the vial sent in contained 4iu.

I also know some of what goes on behind the scenes and have a good idea of where this GH comes from and how it is ordered. Based on that information as well, it does not make any sense that the vial could contain 4iu. Then looking at the literally dozens of serum GH tests and IGF-1's that member of PM have posted, it also defies logic that the GH is underdosed at 4iu. Lastly, you guys have yet to post a lab test of any GH whether it be generic of pharm grade contains anywhere near the correct dose. In fact, they all seem to be off by almost exactly the same multiple. That suggests to me that there may be an error in the calculations somewhere. I believe with your help, believe it or not, I have narrowed that error down to the standard.

Therefore, I am simply asking pertinent questions to find out where the error is. You are correct, I have no idea how to interpret these lab tests and am learning something new about them everyday. I am confident that if we work together we can find out where the error is and correct it for the good of the community. Why is there so much resistance and what do you stand to lose by simply answering questions and helping not only me, but the entire audience, better understand the testing process? If you are correct and there is only 4iu's in each vial, then your answers should only further solidify your position and make it harder to refute and make me look really bad in the end.

In summary, just as you truly believe there is 4iu in the grey tops and other GH's; I truly believe that it is much closer to 10iu. I have outlined the many reasons why I believe this and to me I have always been really good at picking things out that don't make sense; and 4iu's defies logic to me. So can we simply disclose the information that is being requested and move on from here?
 
I can guarantee you that @Dr JIM does NOT sell, profit or gain from any Pharm grade GH. I would bet my life on it.

As far as the sample used for standard with the original tests @CensoredBoardsSuck you can't tell me you aren't following @muscle96ss post and his concerns about the standard AS1625-2 being used as THE Standard. That number is associated with Karl's GH. I sent that sample in initially some time ago and Dr JIM stated that Karl was re-bottling Pharm Grade GH.

All of this that's going on here was supposed to educate MESO and the community. It's only making us here at MESO look like a cluster fuck.

By the way CBS this is my home board and always will be. I've was here way before you came around and I will be here way after you leave. You say I'm so excited to become in some inner circle at PM. I don't try and conform to any inner circles. I'm respected and excepted to many boards because I actually contribute and look out for the community.

mands
The only thing I'm following is the only test result Muscles ever would have accepted is one that gave him the result he wanted. When his objections to the HLC have been answered, he finds something else (and equally ridiculous) to complain about. It is obvious that he will never accept the HPLC.

If you have concerns about the HPLC, why don't you come out and say it? This is your testing thread. You were involved with the testing process from the beginning to the end and you know how it was done, what standards were used, etc., and, more importantly, you know the results are accurate. And yet you have remained completely silent while Muscles tries to destroy its credibility.




No, it's making those who reject the HPLC look like they have an agenda because their objections are silly and frivolous.



And I don't?

Why is it every damn time M-96 becomes involved in a GH thread it all goes to hell and a hand basket?

He did the exact same thing with Ks thread and was clearly not interested in finding the "truth" about Karl's GH, but rather invaded that thread in an attempt to promote K's product in PM.

He almost single handedly destroyed MANDS and my friendship bc of his "shot over the bow" antics and is now attempting to repeat that entire process again.

What has become perfectly clear over the course of this thread, there is only ONE PERSON who has an agenda and it's not myself, CBS or MANDS!!!
 
If the solution (equation used and data breakdown, sample/standard identification, etc) is so clear and simple, and Jim is so wise, why has he not broken it down and explained the alleged discrepancy for the laymen here. Why haven't you helped explain CBS All I see is you try and discredit Muscle96ss. Such an explanation would irrefutably crush the argument if it were done convincingly. Not sure why I haven't seen that yet.

I have.

I'll explain how the mg/mL were determined for the two gray caps tested, even though you could have figured it out yourself.


AS1776-3 GRAY CAP

The formula used was:

{3.72 mg/ml (AS1625-2 rHGH) / x1(peak area)} *x2 (peak area) = y mg/ml

The figures inside the {} are from the Humatrope standard and can be found here.

Using the formula,

{3.72 mg/ml (AS1625-2 rHGH) / x1(peak area)} *x2 (peak area) = y mg/ml

{3.72 mg/ml / 65,493,455} x 25,231,986 = 1.4 mg/ml

y = 1.4 mg/ml



AS1776-1 GRAY CAP

The formula used was:

{3.72 mg/ml (AS1625-2 rHGH) / x1(peak area)} *x2 (peak area) = y mg/ml

The figures inside the {} are from the Humatrope standard and can be found here.

Using the formula,

{3.72 mg/ml (AS1625-2 rHGH) / x1(peak area)} *x2 (peak area) = y mg/ml

{3.72 mg/ml / 65,493,455} x 24,773,978 = 1.4 mg/ml

y = 1.4 mg/ml



AS1625-2 rhGH HUMATROPE STANDARD

{3.72 mg/ml (AS1625-2 rHGH) / x1(peak area)} *x2 (peak area) = y mg/ml

{3.72 mg/ml / 65,493,455} x 65,493,455 = 3.72



As you can see, there were no errors with the mathematics, the chemist didn't use the "wrong equation," and nothing was hidden or withheld. The results are correct.

The question now becomes whether or not you will accept the results or try to find another way to discredit the test. Obviously, that was a rhetorical question.:rolleyes:
 
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