Masteron Increasing Total T According To Blood Work

[Muscleaddict69]

I would really appreciate a response from a doctor or someone who is professionally qualified to answer this. But anyone can give their 2c. I know that all anabolic steroids are suppressive on the HPTA, but I have just heard from a medical doctor who has specialised in Sport's Medicine that masteron can increase Total T! This doctor did a thesis on anabolic steroids use in athletes so it is a subject he is very familiar with.

If this is the case someone using low dose clomid treatment to maintain normal T levels could use low dose masteron without suppression? Everything I know tells me this is not true, but I am not a doctor and am open minded to anything.

The blood work I am referring to shows Total T of 31.4 nmol/L for someone who has been using 300mg/week of masteron for the past 8 weeks. The subject is using no testosterone, SERMs or HCG. The doctor says that this blood work is to be expected for someone on masteron!?

I know that masteron decreases SHBG so if one is on TRT an increase in Free T would be normal. But how could masteron increase Total T, specifically in someone who is not using any exogenous testosterone or HCG? Masteron should be suppressive on endogenous T production?
Edit Post Reply Reply With Quote http://www.allthingsmale.com/forum/report.php?p=228372

 

[Northern Nutrition]

This is a cut and paste from Dr. John Crisler's forum, "All Things Male." What answers did you receive there?

 

[Muscleaddict69]

What's wrong with asking the same question on a different forum? I got no response there. The topic was locked there because they don't want to discuss AAS related topics.

 

[Michael Scally MD]

Masteron will not increase serum testosterone unless there is cross-reactivity, contamination, or some other problem. With LC/MS/MS, there should be NO increase in serum testosterone. Many RIA have reported problems. IIRC, one RIA even measured ND as T. This was posted in the Steroid Forum.

The following is a recent study on the cross-reactivity. There is a push to have all steroid hormones done by MS.

Krasowski MD, Drees D, Morris CS, Maakestad J, Blau JL, Ekins S. Cross-reactivity of steroid hormone immunoassays: clinical significance and two-dimensional molecular similarity prediction. BMC Clin Pathol 2014;14:33. http://www.biomedcentral.com/1472-6890/14/33

BACKGROUND: Immunoassays are widely used in clinical laboratories for measurement of plasma/serum concentrations of steroid hormones such as cortisol and testosterone. Immunoassays can be performed on a variety of standard clinical chemistry analyzers, thus allowing even small clinical laboratories to do analysis on-site. One limitation of steroid hormone immunoassays is interference caused by compounds with structural similarity to the target steroid of the assay. Interfering molecules include structurally related endogenous compounds and their metabolites as well as drugs such as anabolic steroids and synthetic glucocorticoids.

METHODS: Cross-reactivity of a structurally diverse set of compounds were determined for the Roche Diagnostics Elecsys assays for cortisol, dehydroepiandrosterone (DHEA) sulfate, estradiol, progesterone, and testosterone. These data were compared and contrasted to package insert data and published cross-reactivity studies for other marketed steroid hormone immunoassays. Cross-reactivity was computationally predicted using the technique of two-dimensional molecular similarity.

RESULTS: The Roche Elecsys Cortisol and Testosterone II assays showed a wider range of cross-reactivity than the DHEA sulfate, Estradiol II, and Progesterone II assays. 6-Methylprednisolone and prednisolone showed high cross-reactivity for the cortisol assay, with high likelihood of clinically significant effect for patients administered these drugs. In addition, 21-deoxycortisol likely produces clinically relevant cross-reactivity for cortisol in patients with 21-hydroxylase deficiency, while 11-deoxycortisol may produce clinically relevant cross-reactivity in 11beta-hydroxylase deficiency or following metyrapone challenge. Several anabolic steroids may produce clinically significant false positives on the testosterone assay, although interpretation is limited by sparse pharmacokinetic data for some of these drugs. Norethindrone therapy may impact immunoassay measurement of testosterone in women. Using two-dimensional similarity calculations, all compounds with high cross-reactivity also showed a high degree of similarity to the target molecule of the immunoassay.

CONCLUSIONS: Compounds producing cross-reactivity in steroid hormone immunoassays generally have a high degree of structural similarity to the target hormone. Clinically significant interactions can occur with structurally similar drugs (e.g., prednisolone and cortisol immunoassays; methyltestosterone and testosterone immunoassays) or with endogenous compounds such as 21-deoxycortisol that can accumulate to very high concentrations in certain disease conditions. Simple similarity calculations can help triage compounds for future testing of assay cross-reactivity.

 

[Northern Nutrition]

What's wrong with asking the same question on a different forum? I got no response there. The topic was locked there because they don't want to discuss AAS related topics.

Nothing wrong with that at all. It was simply an observation. Anyway, i believe Dr. Scally answered your question.

 

[Muscleaddict69]

Thanks a lot for the response doc. Much appreciated.

 

[Dr JIM]

Excellent post OP bc it's raise an important issue, which is the ACCURACY of tests used to determine the QUANTITY of an AAS or Testosterone in blood.

OP did you understand the reason (point worth emphasizing) serum TT levels may "INCREASE" whenever ANY other AAS is used?

The tests being used IS NOT ACCURATE enough to distinguish between the AAS and natty Test on a structural basis chemically!

These can ALSO be a problem when attempting to QUANTIFY E-2 levels!

So if you really want to KNOW what your natty Test level is, a LC/MS (Liquid Chromatograph /Mass Spec) test MUST be ordered RATHER than an Immuno-Assay.

The latter are often collectively referred to as ELISA tests.

Now that's not suggest every hormone test one conducts requires the specificity of LC/MS, they do NOT.

Primarily bc for most folk a range is adequate as is ELISA. Moreover ELISA tests have come A LONG WAY since their introduction many years ago.

A gander at the cross reactivity patterns in the study above emphasizing how structurally SIMILAR most compounds will need to be for ESILA to be inaccurate

 

[Dr JIM]

For EXAMPLE bc the difference bt E-1, E2 and E-3 is a single molecule of oxygen or it's placement, ELISA is essentially worthless IF you want an accurate ESTRADIOL (E-2) level.

It simply can NOT differentiate between E-1, E-2 and E-3 and LC/MS must be performed.

However if one only needs to obtain a total estrogen level ELISA will work fine.

Regs
Jim