MESO "Specialized" Testing Group Fund

[Kralteramon]

ill throw down 100+ 5x(up to 50)of whatever @Ghoul donates for the dumb fucking NMR test

So just 100 dollars from me


The trailer park boys are here to get nasty

Come on @Ghoul . This is your chance to show that you really care about science and the community. Don't be silent now.

 

[Kralteramon]

No, only some of the outsourced tests. We've not found a replacement yet.

Do you have a price indication for a HGH bio assay?

 

[readalot]

What you mean by this? You wont be offering your services after one month?

See here...

Jano offered an outside lab for CD work.

 

[Spaceman Spiff]

Come on @Ghoul . This is your chance to show that you really care about science and the community. Don't be silent now.

Aggregates, filters, accufine, Imodium, cloudy, folded, kebabs, vacuum, trailer trash

Don't worry bro, he will be her shortly. I used the summoning words

 

[readalot]

No, only some of the outsourced tests. We've not found a replacement yet.

Thank you for the update. I appreciate your partnership as we try to identify competent labs to help answer some of these outstanding questions.

 

[BigDadd7]

How much do we need to run this? Let's get the funds and do it quick while we still can.

 

[readalot]

How much do we need to run this? Let's get the funds and do it quick while we still can.

If the group decides CD is worthwhile then fyi:

The price Jano quoted me was 400 USD for a pair wise comparison with CD and 600 USD for 3 samples. He stated this was no markup on his part with outside lab. I want to speak with this lab first if we go with CD to confirm near vs far UV and make sure it isn't a mess like the oil based endotoxin analyses.

Unsure if we have time logistically with this new info.

I'm excited to see what the group prioritizes and decides.

@Sampei
What crypto currency do you recommend for the fund? Security/privacy vs lowest barrier to participation.

 

[BamaCrazy]

If the group decides CD is worthwhile then fyi:

Unsure if we have time logistically with this new info.

I'm excited to see what the group prioritizes and decides.

@Sampei
What crypto currency do you recommend for the fund? Security/privacy vs lowest barrier to participation.

We can easily come up with $600 or more

 

[readalot]

We can easily come up with $600 or more

Appears we already have more than that. I'll provide my pledge and info I have collected. Will happily defer to Team on what we do testing wise.

 

[readalot]

While we are fundraising and summoning...

===
High frequency word list:
Cuck
STFU
Clown
Loser
POS
Wife
Old Lady
48" vertical
Athletic
Muscular
Reta

Bonus time:
Lying pussy ass bitch
Rat
Lying pussy ass rat bitch

MESO Motivational Speech HOF:

Dude you’re a pathetic fucking loser , scum of the earth , they should of put the taser to every inch of your body , inside your eyes , ass , mouth , everywhere till you were completely paralyzed and in a wheel chair for life . You’re a waste of space on this planet

===

Make it rain Big Dog!!!!

 

[Kralteramon]

Appears we already have more than that. I'll provide my pledge and info I have collected. Will happily defer to Team on what we do testing wise.

Let's find out the price of the test and use that as a the fundraising goal?

 

[readalot]

Let's find out the price of the test and use that as a the fundraising goal?

See post #28 if you are referring to CD test.

I don't have lab options for the bioassays yet. Hopefully we can get there.

Maybe an option?
I haven't reached out to the PI on the study:

R

Post in thread 'The generic vs pharma GH discussion'

Jul 10, 2025

Timely to our discussions on the need to evaluate UG GH using bioassays. Comparison of the methods below (see supplement for more info). This may be a big lift for our MESO testing team to do but would be the money shot IMO (similar to HPLC testing for Testosterone product vs using blood work). Would complement the serum GH testing after IM injection of somatropin.

An improved reporter gene assay for evaluating the biological activity of recombinant human growth hormone

https://www.sciencedirect.com/science/article/pii/S2095177924001709

Nice info in the supplementary data...

• readalot

• 

 

[BamaCrazy]

While we are fundraising and summoning...
===
Cuck
STFU
Clown
Loser
POS
Wife
Old Lady
48" vertical
Athletic
Muscular
Reta
===

Make it rain Big Dog!!!!

If these are nicknames for multiple members we will have plenty funding. Haha

 

[Kralteramon]

See post #28 if you are referring to CD test.

I don't have lab options for the bioassays yet. Hopefully we can get there.

Maybe an option?
I haven't reached out to the PI on the study:

R

Post in thread 'The generic vs pharma GH discussion'

Jul 10, 2025

Timely to our discussions on the need to evaluate UG GH using bioassays. Comparison of the methods below (see supplement for more info). This may be a big lift for our MESO testing team to do but would be the money shot IMO (similar to HPLC testing for Testosterone product vs using blood work). Would complement the serum GH testing after IM injection of somatropin.

An improved reporter gene assay for evaluating the biological activity of recombinant human growth hormone

https://www.sciencedirect.com/science/article/pii/S2095177924001709

Nice info in the supplementary data...

• readalot

• 

Okay. I guess a CD analysis is close enough for what we want to know. Would it also detect folding? It still won't definetively say how the HGH will bind with receptors would it?

 

[readalot]

Okay. I guess a CD analysis is close enough for what we want to know. Would it also detect folding? It still won't definetively say how the HGH will bind with receptors would it?

Properly done CD will give us info on secondary and perhaps tertiary structure of the GH. Hard to say without taking with lab about their capability.

We would need a bioassay to qualify IU/mg (aka bioactivity). My original hallucination was doing (1) CD and (2) bioassay to compare UG samples vs Pharma control (e.g., Serostim).

Super wish list ---> (3) Host cell protein comparison between UG and Pharma.

Practical? Probably not.

@AlexDavis43 is probably right that (2) and (3) enters into expensive CRO (contract research org) territory.

Regardless, just laying out tests that would help answer some of the historical unaswered questions.

 

[Standfast]

Aggregates, filters, accufine, Imodium, cloudy, folded, kebabs, vacuum, trailer trash

Don't worry bro, he will be her shortly. I used the summoning words

You left out filthy mex#@*+

 

[ImSW1]

Don't know s#*t about any of this but I'm in for $100.00 when you have the donation process locked down.

 

[Photon]

What is the goal here?
For science?
Finding one thats closest to pharma?
Or are we trying to pressure UGL HGH manufacturers to do something?
- What are the odds of it happening?

 

[Sera]

@readalot look up ‘Anfinsen’s dogma’

I think CD is a waste of money.

 

[readalot]

The Anfinsen Dogma: Intriguing Details Sixty-Five Years Later

https://www.mdpi.com/1422-0067/23/14/7759

Abstract
The pioneering experiments of Anfinsen on the oxidative folding of RNase have been revisited discovering some details, which update the statement of his dogma and shed new light on the leading role of the correct disulfide in the attainment of the native structure. CD analysis, mass spectrometry, fluorescence spectroscopy and enzyme activity indicate that native disulfides drive the formation of the secondary and tertiary structures that cannot be entirely formed in their absence. This opposes a common opinion that these structures are first formed and then stabilized by the native disulfides. Our results also indicate that a spontaneous re-oxidation of a reduced RNase cannot produce a complete recovery of activity, as described by many textbooks; this can be obtained only in the presence of a reshuffling solution such as GSH/GSSG