R
readalot
Guest
Thanks for sharing your thoughts on what we should go after. So just focus on lab that can quantify IU/mg for each sample? HCPs?
MESO-Rx
Anabolic Steroids
Kralteramon
Member
I would say to benchmark against pharma HGH and confirm biological activity and structural integrity. Maybe even find a connection with side effects.
R
readalot
Guest
Also, no value in methodically checking UG samples for structure prior to bioactivity measurements?
Any concern with presence of inclusion bodies in UG GH and recovery of bioactive protein from them?
Would structural analysis give us additional info we may miss from the "dimer" testing via SEC?
Nice work up demonstrating the methods we are discussing here:
Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent - Microbial Cell Factories
Background Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH...
microbialcellfactories.biomedcentral.com
To be clear, my understanding of this subject is pretty surface level. I think I’m probably wrong.. I mean after looking into it more and looking at that paper you shared it seems you actually can get misfolds especially with the disulfide bridges. And it seems CD can show an average of the structure of the hgh monomers present. That sounds like it could be worth it for what we want.
R
readalot
Guest
Hey, thanks for your feedback sincerely. Ain't my area of professional expertise either. Fun to study though.
My bias is to work up the samples methodically so if we get a poor bioactivity result we may know why. Of course we may in fact get good bioactivity.
But practically speaking, if most are happy with their serum GH results then all this is a probably overkill.
I am still fascinated why the cheapest UG GH made me itch. Full disclosure: was not a blinded experiment and no crossover, yet.
Whatever you guys wanna use but just to remember if it's a lot of different cryptos there will be cost involved in swapping it in the end all to stable coin or BTC to pay Jano accordingly
R
readalot
Guest
Also, plug for proper Chaperones in E Coli...
pmc.ncbi.nlm.nih.gov
journals.lww.com
Do UGLs make use of Chaperones or just recover bioactive protein from inclusion bodies?
Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli - PMC
The overproduction of recombinant proteins in host cells often leads to their misfolding and aggregation. Previous attempts to increase the solubility of recombinant proteins by co-overproduction of individual chaperones were only partially ...
pmc.ncbi.nlm.nih.gov
Effect of Twine-arginine Translocation-signaling Fusion... : Advanced Biomedical Research
could be transported to oxidative periplasmic space and culture media to pass the main limitations in E. coli expression system such as misfolding and inclusion body formation. Materials and Methods: To study the effectiveness of signaling sequences and chaperone co-expression on the...
journals.lww.com
Do UGLs make use of Chaperones or just recover bioactive protein from inclusion bodies?
R
readalot
Guest
That's why I was wishfully thinking we could use the stablecoin site that MESO already set up. Haven't heard anything from Admin yet.
Kralteramon
Member
Which products are we going to test? I have two types of HGH that are opposites in terms of side effects for me. My RHR jumps 7 bpm in a few days on QSC 10iu blue tops. Lobster 10iu maybe +1bpm for the dose. Tried SSA 24iu HGH now since a week and it's a bit in between. +3-4bpm RHR. HRV impact follows a similar trend with more impact with QSC. Happy to donate vials if needed.
niqTM
Member
Never heard of that. Sounds like that would be ideal.
Otherwise, a USDC-Sol wallet would be best IMO. Everyone can see what gets moved in and out of it.
R
readalot
Guest
StoopidGoat
New Member
In and watching for further details, happy to contribute.
Tdeck81
New Member
If I ever figure out my coin wallet, I’ll throw in to help the cause. I know, I know, I’m a caveman
I need to test some things and happened to have serostim I can send for the phamra aspect. Although if Jano’s 3rd party will not be an option in a month it may be pointless?
Just pick a coin and make the crew use Trocador. I am in for $50.
AlexDavis43
Member
If you're going to do bioactivity, you do that one first
That is the most expensive way of doing it
If the GH has good bioactivity, then it doesn't matter how it's folded or if it's sticky or whatever the fuck, it works
And this way you only wasted money on one test not two
R
readalot
Guest
I agree. Seems like that window is closing quickly and I don't see broad support for CD testing. Odds are we may need more than 1 iteration to get all the kinks worked out. Hence I would urge caution before we rush into this option with a lab that is changing management / closing, etc.
Thanks for the offer of product.
I am going to explore what's out there for bioassay options and labs.
I have some shitty ZYH GH I can ship Monday to test. About the worst I have seen recently if you want to use some low end product as a comparison point. Happy to donate a kit.
R
readalot
Guest
I appreciate your feedback and thoughts. I don't think the money will be wasted either way (good or bad bioactivity). Hence why I am happy to put money up. I think it is unfortunate some consider a "good" test result as a waste (yes, this happens on both the KD and BB sides).
AlexDavis43
Member
What POS said a "good" test result was a waste? Name names
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