MESO "Specialized" Testing Group Fund

[readalot]

The Anfinsen Dogma: Intriguing Details Sixty-Five Years Later

https://www.mdpi.com/1422-0067/23/14/7759

Abstract
The pioneering experiments of Anfinsen on the oxidative folding of RNase have been revisited discovering some details, which update the statement of his dogma and shed new light on the leading role of the correct disulfide in the attainment of the native structure. CD analysis, mass spectrometry, fluorescence spectroscopy and enzyme activity indicate that native disulfides drive the formation of the secondary and tertiary structures that cannot be entirely formed in their absence. This opposes a common opinion that these structures are first formed and then stabilized by the native disulfides. Our results also indicate that a spontaneous re-oxidation of a reduced RNase cannot produce a complete recovery of activity, as described by many textbooks; this can be obtained only in the presence of a reshuffling solution such as GSH/GSSG

 

[readalot]

@readalot look up ‘Anfinsen’s dogma’

I think CD is a waste of money.

Thanks for sharing your thoughts on what we should go after. So just focus on lab that can quantify IU/mg for each sample? HCPs?

 

[Kralteramon]

What is the goal here?
For science?
Finding one thats closest to pharma?
Or are we trying to pressure UGL HGH manufacturers to do something?
- What are the odds of it happening?

I would say to benchmark against pharma HGH and confirm biological activity and structural integrity. Maybe even find a connection with side effects.

 

[readalot]

@readalot look up ‘Anfinsen’s dogma’

I think CD is a waste of money.

Also, no value in methodically checking UG samples for structure prior to bioactivity measurements?

Any concern with presence of inclusion bodies in UG GH and recovery of bioactive protein from them?

Would structural analysis give us additional info we may miss from the "dimer" testing via SEC?


Nice work up demonstrating the methods we are discussing here:

Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent - Microbial Cell Factories

Background Formation of inclusion bodies poses a major hurdle in recovery of bioactive recombinant protein from Escherichia coli. Urea and guanidine hydrochloride have routinely been used to solubilize inclusion body proteins, but many times result in poor recovery of bioactive protein. High pH...

microbialcellfactories.biomedcentral.com

 

[Sera]

Thanks for sharing your thoughts on what we should go after. So just focus on lab that can quantify IU/mg for each sample? HCPs?

To be clear, my understanding of this subject is pretty surface level. I think I’m probably wrong.. I mean after looking into it more and looking at that paper you shared it seems you actually can get misfolds especially with the disulfide bridges. And it seems CD can show an average of the structure of the hgh monomers present. That sounds like it could be worth it for what we want.

 

[readalot]

To be clear, my understanding of this subject is pretty surface level. I think I’m probably wrong.. I mean after looking into it more and looking at that paper you shared it seems you actually can get misfolds especially with the disulfide bridges. And it seems CD can show an average of the structure of the hgh monomers present. That sounds like it could be worth it for what we want.

Hey, thanks for your feedback sincerely. Ain't my area of professional expertise either. Fun to study though.

My bias is to work up the samples methodically so if we get a poor bioactivity result we may know why. Of course we may in fact get good bioactivity.

But practically speaking, if most are happy with their serum GH results then all this is a probably overkill.

I am still fascinated why the cheapest UG GH made me itch. Full disclosure: was not a blinded experiment and no crossover, yet.

 

[Sampei]

If the group decides CD is worthwhile then fyi:

Unsure if we have time logistically with this new info.

I'm excited to see what the group prioritizes and decides.

@Sampei
What crypto currency do you recommend for the fund? Security/privacy vs lowest barrier to participation.

Whatever you guys wanna use but just to remember if it's a lot of different cryptos there will be cost involved in swapping it in the end all to stable coin or BTC to pay Jano accordingly

 

[readalot]

Also, plug for proper Chaperones in E Coli...

Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli - PMC

The overproduction of recombinant proteins in host cells often leads to their misfolding and aggregation. Previous attempts to increase the solubility of recombinant proteins by co-overproduction of individual chaperones were only partially ...

pmc.ncbi.nlm.nih.gov

Effect of Twine-arginine Translocation-signaling Fusion... : Advanced Biomedical Research

could be transported to oxidative periplasmic space and culture media to pass the main limitations in E. coli expression system such as misfolding and inclusion body formation. Materials and Methods: To study the effectiveness of signaling sequences and chaperone co-expression on the...

journals.lww.com

Do UGLs make use of Chaperones or just recover bioactive protein from inclusion bodies?

 

[readalot]

Whatever you guys wanna use but just to remember if it's a lot of different cryptos there will be cost involved in swapping it in the end all to stable coin or BTC to pay Jano accordingly

That's why I was wishfully thinking we could use the stablecoin site that MESO already set up. Haven't heard anything from Admin yet.

 

[Kralteramon]

Which products are we going to test? I have two types of HGH that are opposites in terms of side effects for me. My RHR jumps 7 bpm in a few days on QSC 10iu blue tops. Lobster 10iu maybe +1bpm for the dose. Tried SSA 24iu HGH now since a week and it's a bit in between. +3-4bpm RHR. HRV impact follows a similar trend with more impact with QSC. Happy to donate vials if needed.

 

[niqTM]

That's why I was wishfully thinking we could use the stablecoin site that MESO already set up. Haven't heard anything from Admin yet.

Never heard of that. Sounds like that would be ideal.

Otherwise, a USDC-Sol wallet would be best IMO. Everyone can see what gets moved in and out of it.

 

[readalot]

Never heard of that. Sounds like that would be ideal.

Otherwise, a USDC-Sol wallet would be best IMO. Everyone can see what gets moved in and out of it.

R

Post in thread 'MESO "Specialized" Testing Group Fund'

Jul 10, 2025

@MESO-Rx Administrator

Any option to leverage existing infrastructure already on this site for account upgrades?

https://thinksteroids.com/community/account/upgrades

https://pay.cryptocloud.plus/WXPSZG5D

Could we set up a new AnabolicLab type funding system? Have a dashboard with money raised and transparent accounting?

Just brainstorming options. Don't shoot me.

Maybe we want it to be more informal and project based?

• readalot

• 

 

[StoopidGoat]

In and watching for further details, happy to contribute.

 

[Tdeck81]

If I ever figure out my coin wallet, I’ll throw in to help the cause. I know, I know, I’m a caveman

 

[B Ware]

I need to test some things and happened to have serostim I can send for the phamra aspect. Although if Jano’s 3rd party will not be an option in a month it may be pointless?

 

[MadaFacor]

Whatever you guys wanna use but just to remember if it's a lot of different cryptos there will be cost involved in swapping it in the end all to stable coin or BTC to pay Jano accordingly

Just pick a coin and make the crew use Trocador. I am in for $50.

 

[AlexDavis43]

Also, no value in methodically checking UG samples for structure prior to bioactivity measurements?

If you're going to do bioactivity, you do that one first

My bias is to work up the samples methodically so if we get a poor bioactivity result we may know why. Of course we may in fact get good bioactivity.

That is the most expensive way of doing it

If the GH has good bioactivity, then it doesn't matter how it's folded or if it's sticky or whatever the fuck, it works

And this way you only wasted money on one test not two

 

[readalot]

I need to test some things and happened to have serostim I can send for the phamra aspect. Although if Jano’s 3rd party will not be an option in a month it may be pointless?

I agree. Seems like that window is closing quickly and I don't see broad support for CD testing. Odds are we may need more than 1 iteration to get all the kinks worked out. Hence I would urge caution before we rush into this option with a lab that is changing management / closing, etc.

Thanks for the offer of product.

I am going to explore what's out there for bioassay options and labs.

 

[MadaFacor]

I agree. Seems like that window is closing quickly and I don't see broad support for CD testing. Odds are we may need more than 1 iteration to get all the kinks worked out. Hence I would urge caution before we rush into this option with a lab that is changing management closing.

Thanks for the offer of product.

I am going to explore what's out there for bioassay options and labs.

I have some shitty ZYH GH I can ship Monday to test. About the worst I have seen recently if you want to use some low end product as a comparison point. Happy to donate a kit.

 

[readalot]

wasted

I appreciate your feedback and thoughts. I don't think the money will be wasted either way (good or bad bioactivity). Hence why I am happy to put money up. I think it is unfortunate some consider a "good" test result as a waste (yes, this happens on both the KD and BB sides).