My proof of concept for vaccume filtering

[McLovin.]

next up a vacuum cleaner pulling vacuum in the vial pulling the oils through the syringe filter

 

[narta]

Hi Narta can you explain this setup a bit more? i looked up silicone seplas and am not sure of their purpose,

https://a.aliexpress.com/_EvkfcaH

You can sterilize the gl45 glassware and silicone stoper, converting the gl45 receiver as a giant sterile vial. Use a long ass needle to transfer the filtered gear to smaller presterilized vials.

 

[max187]

https://a.aliexpress.com/_EvkfcaH

You can sterilize the gl45 glassware and silicone stoper, converting the gl45 receiver as a giant sterile vial. Use a long ass needle to transfer the filtered gear to smaller presterilized vials.

fucking hell, i am so embarassed. I spent days and days looking to find this exact thing but the only stoppers i could find were from a pharma company, ill link pic below.
Thanks so much Narta you are a gentleman and a scholar.
Would you mind linking the bottle top filters you use? Also what kind of needle do you use to draw from the bottom of big ass GL45 bottles

 

[narta]

fucking hell, i am so embarassed. I spent days and days looking to find this exact thing but the only stoppers i could find were from a pharma company, ill link pic below.
Thanks so much Narta you are a gentleman and a scholar.
Would you mind linking the bottle top filters you use? Also what kind of needle do you use to draw from the bottom of big ass GL45 bottles

View attachment 294758

I don't use bottletops but anything reputable that has a gl45 neck would do. Opt for PTFE hydrophilic with PVDF being a second option. .22microns

As for needles you don't need long ones, just think that the gl45 with the septa is a big ass vial, so just flip and draw

There are long needles but usually they need sterilization between each batch and it's a pita. Search for 100mm dispensing needles

 

[HB_22]

https://a.aliexpress.com/_EvkfcaH

You can sterilize the gl45 glassware and silicone stoper, converting the gl45 receiver as a giant sterile vial. Use a long ass needle to transfer the filtered gear to smaller presterilized vials.

I can get it all pre sterilized here in Germany right from the manufacturer. But to my understanding if I take the bottle top filter off the glass bottle and put the pre sterilized silicone stopper on it there is a small risk of contamination by not using a flow hood, right? Like some unfiltered air is going in during the 2-3 seconds of changing the bottle top filter to a silicone stopper.

Thats the actual reason I am still using vials and syringe filters as I have no place to hide a flow hood this size anywhere, my son will find it and I honestly can not tell if its a risk or not to do it without a flow hood.

 

[max187]

I can get it all pre sterilized here in Germany right from the manufacturer. But to my understanding if I take the bottle top filter off the glass bottle and put the pre sterilized silicone stopper on it there is a small risk of contamination by not using a flow hood, right? Like some unfiltered air is going in during the 2-3 seconds of changing the bottle top filter to a silicone stopper.

Thats the actual reason I am still using vials and syringe filters as I have no place to hide a flow hood this size anywhere, my son will find it and I honestly can not tell if its a risk or not to do it without a flow hood.

I am happy enough to take the chance of a tiny bit of unsterile air getting into the bottle tbh.
You could always mist the air around the bottle top with isopropal alcohol a few seconds before opening for peace of mind?