New Hgh - floaters?

[Photon]

Do you know what Jano uses before every HGH and peptide test he does? Answer: a 0.22um PTFE filter. Moral of the story here is you shouldn't believe everything Ghoul says.

Do you have the link where it says he uses PTFE for all?

I just posted in his thread. I'm curious too.

Post in thread 'Janoshik Analytical laboratory testing services'

Saturday at 7:50 PM

We used to filter only cloudy peptides, but we now filter everything, after we confirmed on thousands of samples, that it does nothing to the result, while preventing expensive maintenance.

Hi Jano

What type of filters do you use for peps?
PVDF vs PES? or PTFE?
Most of us use PES here but i believe some have mentioned PVDF.

Do you see any difference in testing between the other? (or have you just stuck with one?)

• Photon

• 

 

[riggspersonal]

See, this thread is getting somewhere now.

 

[thunkaboutit]

Love when discussion leads to productive thoughts and conversations.
Science iz kewl

Update
I got some sterile vials and syringe filters coming, low protein binding just to be safe. .22 pores
I think purity will remain in tact while getting the floaty bs out.
Vendor reports it may be proteins or mannitol. Ill be honest I did pin like little bit of it (1iu). Its doing something for sure, side effects are felt. I am new to anything outside serm and cjc so take that with grain of salt

Love all of you gay nerds xD


Edit: went with PES hydrophilic (plz advise)

 

[deadbeef]

PTFE or PES?

PTFE

 

[deadbeef]

This study used hydrophobic membranes.

They used PES filters, which for aqueous solutions are hydrophilic.

"When the hydrophobic interface was occupied by surfactant molecules,

You are conflating "hydrophobic interface" with the filter. This is referring to the hydrophobic surfaces on the proteins.

 

[deadbeef]

Do you have the link where it says he uses PTFE for all?

He answered this on discord:

 

[Elektrobot]

You are conflating "hydrophobic interface" with the filter

PTFE is a hydrophobic membrane

They used hydrophobic PTFE filters in that study

 

[deadbeef]

PTFE is a hydrophobic membrane

They used hydrophobic PTFE filters in that study

We are actually looking at different studies, but here is from the study you are looking at:

All filters were 0.22 µm in pore size. Most of the
filtration experiments were performed using MF filters (Millipore,
13 mm in diameter) made of mixed esters of cellulose. Other
materials included nylon (Whatman, 13 mm), polycarbonate,
(Poretics, 25 mm), poly(vinylidene fluoride) (Durapore, 13 mm),
and polysulfone (Gelman, 13 mm). The polycarbonate filter was
a capillary filter, i.e., having randomly distributed cylindrical,
straight-through pores. Others were noncapillary filters. All
membrane filters were flushed with protein buffer before use

No mention of PTFE. And if we let AI look up these filters for us:

* Mixed esters of cellulose (Millipore MF): Hydrophilic.
* Nylon (Whatman): Hydrophilic.
* Polycarbonate (Poretics): Typically hydrophilic or at least easily wettable in water; many commercial PCTE track‑etched membranes are sold as hydrophilic for aqueous filtration.
* PVDF, Durapore (Millipore): Sold in both hydrophilic and hydrophobic versions; the 0.22 µm Durapore used for aqueous protein work is usually hydrophilic PVDF, but there is also a hydrophobic Durapore line.
* Polysulfone (Gelman): The base polymer is intrinsically hydrophobic, and unmodified polysulfone membranes are considered hydrophobic unless surface‑treated.

Given the context (“flushed with protein buffer”), the authors almost certainly used the hydrophilic variants of MCE, nylon, polycarbonate, and Durapore for aqueous protein solutions, while polysulfone would be the most hydrophobic surface in the set unless explicitly modified.

In reality, and as the AI points out, it would make zero sense to use a hydrophobic filter with an aqueous protein solution. These researchers aren't stupid.

Furthermore, many filters, including nominally hydrophilic filters, contain hydrophobic or less-wettable interfacial regions due to chemical and structural heterogeneity. Hydrophilic filters are therefore not perfectly hydrophilic, they often exhibit hydrophobic patches, edges, or pores where water structuring differs from the bulk. Surfactant excipients are added to preferentially occupy these interfaces instead of the protein, which prevents or greatly reduces interface-induced aggregation and membrane fouling.

We have no idea if UGL HGH contains these excipients (but probably not).

 

[deadbeef]

PTFE is a hydrophobic membrane

Post in thread 'Janoshik Analytical laboratory testing services'

Yesterday at 1:19 AM

Hydrophilic PTFE

• janoshik

• 

 

[Elektrobot]

it would make zero sense to use a hydrophobic filter with an aqueous protein solution

This is why I pointed it out

I didn't realize PTFE could be both hydrophobic and hydrophilic . I had only seen hydrophobic PTFE filters

So I wonder what a study like that would look like with a PES filter like the ones most people here use