PEP test-e contaminant testing?

03xx52

New Member
So I have access to a plethora of microbiology equipment and I was thinking about throwing a bit of PEP gear on some TSA, growing medium for most types of bacteria, and seeing what I get. Would anyone be interested? I am in no way shape or form associated with PEP so I think this may be a fair way to test my vials for bacterial contamination.
 
So I have access to a plethora of microbiology equipment and I was thinking about throwing a bit of PEP gear on some TSA, growing medium for most types of bacteria, and seeing what I get. Would anyone be interested? I am in no way shape or form associated with PEP so I think this may be a fair way to test my vials for bacterial contamination.

Awesome. I'm in.
 
I may guinea pig their tbol. It's $100 for 2500mg(2 packs) after discount. First cycle so, albeit it's only tbol, the effects should be more pronounced on my 'virgin receptors'.
 
Good to go, Ill be testing .2 ml from two test E red top vials with test- e...Ill throw them on tryptic soy agar and incubate for 24 hours. We will know by monday.
 
Interested in your findings, also a little nervous, but regardless we all take a risk when injecting substances into our bodies whether it's a ugl or pharma grade.
 
So I have access to a plethora of microbiology equipment and I was thinking about throwing a bit of PEP gear on some TSA, growing medium for most types of bacteria, and seeing what I get. Would anyone be interested? I am in no way shape or form associated with PEP so I think this may be a fair way to test my vials for bacterial contamination.

yes im actually planning bacteria testing with agar in the next little bit as soon as I get some spare time
 
Are you spiking the plates in a BSC II?
Of course, also when I make TSA, since I test neuronal cell lines, I like to mitigate as much contamination of the plates as possible. I pour the TSA in the L flow hood at 55 degrees C and let them harden under 355 nm UV.
 
Of course, also when I make TSA, since I test neuronal cell lines, I like to mitigate as much contamination of the plates as possible. I pour the TSA in the L flow hood at 55 degrees C and let them harden under 355 nm UV.

I think it'd be cool if we were able to get the floaters under the scope.
 
In all reality given the conditions in the vial, I would be very suprised if "clear" oil had microbes in it. The reason being, microbes usually produce various compounds that do not react well with oils. Thus if a vial was contaminated it "should" be milky in color. Lets hope for the best.
 
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