PEP test-e contaminant testing?

[brilliance06]

In all reality given the conditions in the vial, I would be very suprised if "clear" oil had microbes in it. The reason being, microbes usually produce various compounds that do not react well with oils. Thus if a vial was contaminated it "should" be milky in color. Lets hope for the best.

If the bacteria was able to grow in the oil, then it'd turn turbid, right? If the bacteria is dead when placed in the vial, it won't grow on a plate or liquid culture assessment.

EDIT: Unless it's some sort of spore forming bacteria that can't grow in oil, then it could be clear, no?

 

[03xx52]

If the bacteria was able to grow in the oil, then it'd turn turbid, right? If the bacteria is dead when placed in the vial, it won't grow on a plate or liquid culture assessment.

EDIT: Unless it's some sort of spore forming bacteria that can't grow in oil, then it could be clear, no?

Correct, I think,(EDIT: I have never grown bacteria in an oil) spores are somewhat of a possibility because I doubt most UGL's have an autoclave. The suspension could still have remnants of bacterial cells...The human body does not typically like the toxins in dead bacteria which could explain some PIP, I think a pertinent explanation for the high PIP some experience is the high concentration of solute(testosterone). We will see tomorrow, I will be plating 4 plates with .2 ml each.

 

[irishchamp]

Subscribed! Should be interesting. I'm currently running PEPs gear cut with filtered/sterile grape seed oil, no pip to speak of.

 

[digler]

Very interesting , I am running his mast e and it's silky smooth , I just hope it's real .

 

[11BJJ]

Subscribed

 

[dtibs22]

Very interesting , I am running his mast e and it's silky smooth , I just hope it's real .

I don't think there has ever been a question of his gear being "real". Really just possible sterility issues.

I'm also very interested in these results.

 

[digler]

I don't think there has ever been a question of his gear being "real". Really just possible sterility issues.

I'm also very interested in these results.

I haven't heard any either , but I haven't seen any proof his mast being real either . I just question it until I know it's real ! That's all , no attack

 

[03xx52]

Alright we will know something tomorrow morning!

 

[dtibs22]

I haven't heard any either , but I haven't seen any proof his mast being real either . I just question it until I know it's real ! That's all , no attack

Fair enough. I guess as I think about it, haven't heard much about the effectiveness of his mast.

 

[stomps]

Can't wait
In the coming weeks ill be using agar as well
However I have not even the slightest experience as you seem to have

 

[03xx52]

Just agar won't do anything, it has to have nutrients in it...Agar is just a hardening agent try to find a recipe for tryptic soy agar online.

 

[stomps]

Just agar won't do anything, it has to have nutrients in it...Agar is just a hardening agent try to find a recipe for tryptic soy agar online.

Oh yeah I know I was looking at these

[ame=http://www.amazon.com/Blood-Agar-EH-Plate-Pack/dp/B0017U0BV0/ref=sr_1_11?ie=UTF8&qid=1390762451&sr=8-11&keywords=agar+plates]Blood Agar EH Plate (10 per Pack): Amazon.com: Industrial & Scientific[/ame]

 

[stomps]

I've been reading up on about streaking agar
Did you do this if so what was your process

 

[03xx52]

BA might work it more of a differentiating agar but it should be fine. I used a sterile swab and used a syringe to put .2 ml on the agar then the swab to do a three way streak. Just Google three way streak microbiology and it will pop up. It's an easy way to try and get a confluent carpet of bacteria.

 

[stomps]

BA might work it more of a differentiating agar but it should be fine. I used a sterile swab and used a syringe to put .2 ml on the agar then the swab to do a three way streak. Just Google three way streak microbiology and it will pop up. It's an easy way to try and get a confluent carpet of bacteria.

Would you recommend tryptic soy over blood
When I get ready in the next few weeks is it cool if I pm you

 

[drgreenthumb628]

Cant wait to hear results!

 

[brilliance06]

Would you recommend tryptic soy over blood
When I get ready in the next few weeks is it cool if I pm you

Blood agar is just TSA with a certain % of sheep blood.

Blood agar is used a lot more for hemolytic reactions.

 

[MasterStim]

Subscribed, i want to see the results too.

 

[integralbasic]

Yep no pip what so ever with mast e, not that that's an indicative or its legitimacy

 

[Dr JIM]

The only means of determining a cause and effect relationship using any culture technique is to recover the same organism in a patients wound or abscess.

But I must mention there is NO EVIDENCE that supports the notion bacterial gear contamination causes PIP.

First while PIP is relatively common site infections are NOT.

Second because the responsible organisms are almost always Staph and Strep, without sub-typing, proving the UGL is responsible party, rather than the patients poor technique, approximates the impossible.

I know bro lore dictates the cause of PIP is the "ester" being used but again NOTHING supports this contention either.

So what does cause PIP you ask? Look no further than the AAS powder guys.

Now Jim how can that be after all my source double or triple filters their powders.

Ok right, assuming that does in fact occur (and the better UGLs do exactly that) the etiology is the number of times the final product is distilled, precipitated and reconstituted.

Ya see each time this triple phase process is performed a variable quantity is "lost" in the primary solution, and that lowers the WEIGHT available for SALE.

I'll give one example easily detected using MS or HPLC