Trentok061
New Member
80€-90€. Seems like a good price for starters. And I don't know if I can continue to use the Instapot for sterilization, in case my girlfriend tries it for cooking. Better to get something separate right away.
MESO-Rx
Anabolic Steroids
Pressure cooker for steriliIzing
- Thread starter Sampei
- Start date
I forgot.
If you are using a pressure cooker for sterilization, then at least use a sterility indicator. If you are not using sterilization pouch bags with an indicator.
euromedis.eu
There are similar ones for those who sterilize in the oven.
If you are using a pressure cooker for sterilization, then at least use a sterility indicator. If you are not using sterilization pouch bags with an indicator.
Sterilization Indicator Labels 500 pieces
Labels with double adhesive backing with process indicator for autoclave steam sterilization. The products are used for people/patients related traceability of all medical sterilization processes (dentists, hospital, laboratories,…)
euromedis.eu
narta
Member
Get a replacement inner pot
MediumRawSteak
New Member
Just gonna jump in here, A few things I thought would be helpful here from having some past experience with home sterilization from making my own nutrient blends for MMJ & Mycology I thought this chart would be helpful to those who can’t reach 15psi., I used to do 9psi for 2.5hrs to achieve sterilization that actually was for sure sterile in an Instapot.
You can sterilize something at say…11psi but it’ll take more time inside the cooker/autoclave to be sterile but it will sterilize. The 23qt Presto I’ve used for years to sterilize liquids, grains, and PP5 Petri Dishes is overkill and I used an Instapot for almost a year before switching over and had no contamination outbreaks in my 2 tent systems for weed or in my 18oz mason jars of nutrient blends or 10mL syringes filled with living liquid cultures of (legal) mushroom varieties. The chart listed is for Grain Spawn for mushrooms so divide the time by ~3 and you’ll have the approx time for equipment sterilization but I’ve been around genuine mycologists who flash sterilize at like 18psi for 10min and say that 15 psi for longer than 30min is wasting time.
Also something I wanted to point out, almost all FFU’s produce Laminar airflow, and if they don’t, the Alibaba brand ones still have enough Horsepower to push the contaminated air past the center-face of the grill and tests show that even with a FFU leaking air around the face without laminar flow, the center is almost always up to standard ~99.93% filtration purity as long as the filter itself is fully intact and within its lifespan or reached/went beyond its usage limit.
I did a test with living tissue culture samples on Petri dishes: one with an open plate of sporulating trichoderma contaminated mushroom cultures on the same tray (trays pictured on sample table) within ~8-12” of another open Petri dish with a non contaminated tissue culture and dropped the uncontaminated into the liquid nutrient and there was no outbreaks of Trich contamination that had to be overcome in the uncontaminated liquid nutrient turning culture over the next 2 weeks. That should give a general idea to how powerful these devices are and since then I’ve upgraded to a TerraUniversal Smart FFU that way I can slap a control unit on there here when the missus allows for a frivolous purchase (and gotten over this one) to sterilize the room while I shower and prep myself before entering the room but seriously, not much difference between the two, in fact I can slap the Terrauniversal HEPA or ULPA filter face on a specific Alibaba FFU model and it’ll screw in all 12 of the holes where the TerraUniversal face will. Just wanted to say my piece on a few things I’ve seen on the homebrew threads, which by the way, thank you for the pioneering. There seems to be some overlap between old hobbies and this new one and am looking forward to seeing what can be applied from one to the other.
That being said, has anyone found Syringe Filters that can be home sterilized? Those are the final part to this that’s driving my OCD crazy; I don’t know if I wanna make enough in one go at the start to use a bottle top filter or go Syringe Filter and if I do go syringe filter has anyone found Acrylic housing PTFE hydrophobic .22um filters or would you guys even consider it worth buying unsterile then trying to sterilize them?
Thanks for coming to my PEDtalk lol.
TL;DR if you wanted to go all out for sterility, there wouldn’t be a need other than the Alibaba FFU model that has the same screw alignment as the Terrauniversal models, a TerraUniversal 2’x4’ HEPA Filter face to swap onto the Alibaba and a Presto 24qt pressure canner with a ghetto floor made from a few screws at Home Depot to raise the floor plate and can buy the 2x steel separation plates together, put one on top of whatever you’ve got inside to make sure that if something goes wrong inside the PC, the upper plate will be there to stop the obstruction from blocking the air vent and emergency vent stopper. Hope this helps for pressure cooker sterilization.
You can sterilize something at say…11psi but it’ll take more time inside the cooker/autoclave to be sterile but it will sterilize. The 23qt Presto I’ve used for years to sterilize liquids, grains, and PP5 Petri Dishes is overkill and I used an Instapot for almost a year before switching over and had no contamination outbreaks in my 2 tent systems for weed or in my 18oz mason jars of nutrient blends or 10mL syringes filled with living liquid cultures of (legal) mushroom varieties. The chart listed is for Grain Spawn for mushrooms so divide the time by ~3 and you’ll have the approx time for equipment sterilization but I’ve been around genuine mycologists who flash sterilize at like 18psi for 10min and say that 15 psi for longer than 30min is wasting time.
Also something I wanted to point out, almost all FFU’s produce Laminar airflow, and if they don’t, the Alibaba brand ones still have enough Horsepower to push the contaminated air past the center-face of the grill and tests show that even with a FFU leaking air around the face without laminar flow, the center is almost always up to standard ~99.93% filtration purity as long as the filter itself is fully intact and within its lifespan or reached/went beyond its usage limit.
I did a test with living tissue culture samples on Petri dishes: one with an open plate of sporulating trichoderma contaminated mushroom cultures on the same tray (trays pictured on sample table) within ~8-12” of another open Petri dish with a non contaminated tissue culture and dropped the uncontaminated into the liquid nutrient and there was no outbreaks of Trich contamination that had to be overcome in the uncontaminated liquid nutrient turning culture over the next 2 weeks. That should give a general idea to how powerful these devices are and since then I’ve upgraded to a TerraUniversal Smart FFU that way I can slap a control unit on there here when the missus allows for a frivolous purchase (and gotten over this one) to sterilize the room while I shower and prep myself before entering the room but seriously, not much difference between the two, in fact I can slap the Terrauniversal HEPA or ULPA filter face on a specific Alibaba FFU model and it’ll screw in all 12 of the holes where the TerraUniversal face will. Just wanted to say my piece on a few things I’ve seen on the homebrew threads, which by the way, thank you for the pioneering. There seems to be some overlap between old hobbies and this new one and am looking forward to seeing what can be applied from one to the other.
That being said, has anyone found Syringe Filters that can be home sterilized? Those are the final part to this that’s driving my OCD crazy; I don’t know if I wanna make enough in one go at the start to use a bottle top filter or go Syringe Filter and if I do go syringe filter has anyone found Acrylic housing PTFE hydrophobic .22um filters or would you guys even consider it worth buying unsterile then trying to sterilize them?
Thanks for coming to my PEDtalk lol.
TL;DR if you wanted to go all out for sterility, there wouldn’t be a need other than the Alibaba FFU model that has the same screw alignment as the Terrauniversal models, a TerraUniversal 2’x4’ HEPA Filter face to swap onto the Alibaba and a Presto 24qt pressure canner with a ghetto floor made from a few screws at Home Depot to raise the floor plate and can buy the 2x steel separation plates together, put one on top of whatever you’ve got inside to make sure that if something goes wrong inside the PC, the upper plate will be there to stop the obstruction from blocking the air vent and emergency vent stopper. Hope this helps for pressure cooker sterilization.
Thanks mate, fantastic information.
MediumRawSteak
New Member
Thanks man, ya the raised bottom keeps water out of the vessels and an air pump attached to a cylindrical filtration system attached to an motorized air pump is what I’ve used to blast away micro particulates that have been cooked onto the vials to prevent clouding from heat cycling the flasks/beakers with the inert material.
For chemical sanitation of a work station I personally like a Bleach coat, wipe down followed by a light 71% Iso coating but make sure everything you’re going to be using has been lifted where contact would obstruct the iso from sanitation and hand wiped down with fiber less “shop towels” then let the 71% solution stay and dry into distilled water and evaporate and finish with a solution called “In-Cide” and again, I’ve watched professionals in multimillion dollar labs start with In-Cide, some 71% Iso, and ending with In-Cide cleaner.
It’s my goto cleaner for OCD level cleaning but when my stations are set up I only hit the room with In-Code every 4 months.
Ps forgot that the mushroom on the table was a chonker that had pictures taken of it.
AliExpress: ￡61.65 | Dust Cage Electric Dust Hood Enclosed High Performance Acrylic Dust Box Cover for Dust Box Grinding Machine Grinding Table
But change filter
But change filter
MediumRawSteak
New Member
SAB’s are great for field work when biological microorganisms are in their natural environment but sterility is still needed to capture/transfer/collect the sample.
We used these a lot for lakes and rivers when doing runoff tests in national parks and popular hiking/survival camping spots to make sure when people go to “be one with nature” they aren’t drinking arsenic & mercury or on the better side, shit and piss when they go to the river to collect their cooking or drinking water.
They don’t have very good sterility unless you’re already in an environment designed around sterility but like the model shown they have added outlets to hook up a continual airflow of sterility.
I swear I’ll recover from this Thanksgiving lump in my stomach soon and step into the “cleanroom” where I have 2 neat work arounds for FFU’s or if you’re a woodworker, a literal FlowHOOD!
I almost wanna ask my old professor if he’d be open to joining this since he was very finge with his thinking and ideologies.
I’d buy that SAB if I was still in the field and one wasn’t provided.
narta
Member
You are overanalyzing shit.
MediumRawSteak
New Member
I’m sure but if the tools are already available, it’s just an advanced sterile procedure.
Edit: there also seems to be two very opposing sides to “sterility” when in all reality there’s a huge difference between “sterile” and “sanitized” and “sanitizing is mainly what I’m seeing in these threads…
As long as it’s not taking immense time or effort why not use extra caution?
narta
Member
Because after decades of shitty ugl gear, injected by thousands of people, millions of times (and I am being conservative with the numbers), the reported severe infections that led to serious trauma are pretty close to ZERO.
Doing the extra 1% that takes an additional investment of 10% and an additional 10% time to setup each time, in order to reduce 0.0000001% to 0.000000005% is not necessary
MediumRawSteak
New Member
Hey dude, that’s your call, I’m just here to share some info.
Like for example, you specifically keep using the term “sterile” when you’re actually doing a “sanitation” technique with the 3% chlorine option…
But again, your choice to purchase the items or not lol, I’m not forcing you dude.
narta
Member
It is definitely the end user's call, but I am here just to share my opinion, that overanalyzing shit is retarded, in a practical sense.
Theoretically speaking, your process is better. Practically it's just more lengthy, Because if you don't have HEPA air filters that control the quality of air it enters the room and positive pressure, you don't have a truly sterile environment
iris
Well-known Member
U found another bubble boy, honey?
And u so chill about the paralysis by analysis.
Either in a good mood or knackered.
Cute.
Please do NOT go find a "F Off" gif to chuck at me.
MediumRawSteak
New Member
If we’re talking in theoretical or practicality a FFU/Flowhood/Clean Station in a home is never going to be up to the standard it’s designed for so that point is moot but ok…there’s a cutout behind the slatted shade to pull in fresh air from an attached filtration tube outside.
It’s all subjective dude, you dont have to use the advice but retarded is a bit harsh, I could definitely point out some retarded posts you’ve given on these subs for “sterile” advice when it was “sanitary” advice but you don’t gotta be a dick dude.
I appreciate the other advice you’ve given on other subs.
narta
Member
It's not subjective. Either it's sterile or not. Sterile enough, does not exist.
Well, Your sterile is more sanitary than my sanitized, by your standards.
That is what I argue about. Once you are close to sterility aka autoclaved/dry heat the media and vials/stoppers/transfer equipment, implementing another step that gives an extra 0.001% sterility, for the lack of a better term, it's retarded from a practical standpoint, because you have diminishing returns on investment (time, funds, space)
I know your intentions are good, your knowledge for pressure cooking sterilization is excellent and that you are driven by your passion. But we need to abstain from paralysis by analysis.
MediumRawSteak
New Member
Going in reverse, I can absolutely get behind the “KISS” standard of thought,, and you’re right, I go off of the sterility standards I was taught in a fully different environment and context so that’ll be a personal challenge to find that line for myself.
I think we both saying the same thing in two different languages for the most part but I’ll take your word that anything above standard sterilization techniques is overkill.
I did want to just make sure the info is out there though that sterilization and sanitation are actually separate processes so to a degree but they have slightly different (or major, depending on the context) *end goals
Sanitization is done with the goal to minimize the amount of contaminates to a manageable level for the medium that it’s being used for to be used safely
But you are fully correct that something is either sterilized* or it’s not with the end goal of microbial death and creating inert particles with sterilization, usually through a heat cycling process.
No matter, I see it now but come on! Don’t we all want UVC lasers that’ll blind us in 30yrs when we need the Cialis for boners and hydrocarbon air pumps to get hot and heavy hahaha. For me it’s just learning where the metaphorical “edge” for what’s needed to achieve sterility then backing down the safety standards to have efficiency meet efficiency, for me at least.
Thanks for the understanding dude.
Edited: *missing trains of thought & grammar
I think we both saying the same thing in two different languages for the most part but I’ll take your word that anything above standard sterilization techniques is overkill.
I did want to just make sure the info is out there though that sterilization and sanitation are actually separate processes so to a degree but they have slightly different (or major, depending on the context) *end goals
Sanitization is done with the goal to minimize the amount of contaminates to a manageable level for the medium that it’s being used for to be used safely
But you are fully correct that something is either sterilized* or it’s not with the end goal of microbial death and creating inert particles with sterilization, usually through a heat cycling process.
No matter, I see it now but come on! Don’t we all want UVC lasers that’ll blind us in 30yrs when we need the Cialis for boners and hydrocarbon air pumps to get hot and heavy hahaha. For me it’s just learning where the metaphorical “edge” for what’s needed to achieve sterility then backing down the safety standards to have efficiency meet efficiency, for me at least.
Thanks for the understanding dude.
Edited: *missing trains of thought & grammar
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