Pressure cooker for steriliIzing

[Oxand]

@ThinkTrenbolone what temp do you have your oven on when you throw the vials in with the autoclave bags and for how long bro?

 

[Sampei]

@ThinkTrenbolone what temp do you have your oven on when you throw the vials in with the autoclave bags and for how long bro?

Just 50c is more then enough it's only to dry the humidity left

 

[Oxand]

Just 50c is more then enough it's only to dry the humidity left

Awesome thanks bro

 

[egruberman]

This is the one I was checking out on AliExpress 120KPA looks well done but I have no fucking clue to be honest

Looks.... Okay. I'd be concerned about that valve. If it fails, there are no parts.

Most of the failures of those things are from the fact that one cooks meat or beans or whatever in them and food matter gets stuck in the valve, so maybe fine for your case.

 

[jankoch]

Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications

The sterilization processes of nanoparticles (NP) by autoclaving and filtration are two of the most utilized methods in the pharmaceutical industry but are not always a viable option. For this reason, the search for alternative options such as UV and ...

www.ncbi.nlm.nih.gov

It talks about UVC light for sterilzing.

ULTRAFIALOVÁ GERMICÍDNA STERILIZAČNÁ LAMPA 32W UV-C AIR SANITIZER LEDVANCE - ledziarovka.eu

ULTRAFIALOVÁ GERMICÍDNA STERILIZAČNÁ LAMPA 32W vhodná pre alergikov

www.ledziarovka.eu

Could make a box for it and put all the vials etc in it to have concentrated UVC exposure. Not sure how much heat it produces so It might be wise to monitor temp with such a small space.

After covid I bought 2 of this in a supermarket. Original price was 60 euro, and it was off 90%, so got it for 6 eur lol.

What if no pressure cooker, but:
1. standard rinsing (dishsoap + water, then isopropyl bath, rinsing with sterile water)
2. UVC light with disassembled vials+ stoppers (60mins)
3.
4. Philips Baby bottle sterilizer with drying function, maybe 2 rounds (1 round is 45 mins with drying)
5. another round of UVC light this time with assembled vials, so no more contaminant can get in, just the light.

A) is it a viable option having no pressure cooker that is capable 15psi+?
B) what about sterilizing the finished product for less time with the UVC light? Does it harm the standard test C brew for example?

 

[narta]

Most commercial uvc lights don't penetrate regular glass.

 

[bigtom343]

Also keep in mind, most people who are sterilizing glass want it to look clean. So either dry sterilization (oven or toaster oven) or an autoclave WITH A DRYING cycle option. Most cheap autoclaves don’t have a drying cycle. This led me to seek a name brand autoclave from a legitimate medical supply company by using a friend who has medical credentials and a vets office to hide my identity.


Your best bet if you have limited options is a large toaster oven and use it ONLY for sterilization. You can use a loose aluminum foil tent to cover any openings in glassware. This is the method I’ve used for years and it’s worked great.

The brand new toaster is a great idea. I could buy one and send it back to Amazon every few months and exchange a new one. I have access to a fancy autoclave at a research center but it still seems to leave some question of cleanliness because I lack full control of the autoclave and it’s immediate surroundings. Also even after it’s finished with the drying cycle there is some residual moisture trapped inside of the vials so I have to transfer to a secondary oven to get it completely dry. The glass looks shiny tho.

 

[bigtom343]

First dish soap. Then alcohol bath. Then distilled water. Then one more bath if alcohol. Then if I have it. Ethanol that I light on fire after most is evaporated.

Then straight into autoclave bags with gloves. Sealed. Pressure cook. Then dry out in oven in the sealed auto clave bags at low temp to get rid of any moisture from the inside if it exists at all

That’s a lot of steps! My QM used to tell me cleanliness is godliness!

 

[HB_22]

Yeah but those are American brands and a research done in America. We don't have canned/pressure cooker culture here in Europe, it's like none of the normal available pressure cooker goes to 15psi lol

The instant pot, the pressure cooker that tested the best in the linked study, is widely available even in the EU. You can buy it on Amazon for 150-190€.

 

[Sampei]

The instant pot, the pressure cooker that tested the best in the linked study, is widely available even in the EU. You can buy it on Amazon for 150-190€.

I must have missed it, can you link to me the model? thanks man!

 

[bighunanballs]

Yeah just get an instapot if that's available in your country it's got the heater mechanism built into it It doesn't sit on the stove top

 

[HB_22]

I must have missed it, can you link to me the model? thanks man!

Here it is available in Germany, but also in other countries from Amazon. If you wait for "Black Friday" deal or any other special promo it is available for 120-130€.

It even has the "sterilize" function as a menu point.

Amazon.de

 

[Sampei]

Here it is available in Germany, but also in other countries from Amazon. If you wait for "Black Friday" deal or any other special promo it is available for 120-130€.

It even has the "sterilize" function as a menu point.

Amazon.de

The pro plus WiFi I have checked reach 15psi the only one of the latest instant pot . I'm waiting to come back in stock in the Italian website

Now I'm gonna need some fucking media bottle and silicone septa cap and a vacuum pump too, shit I haven't even started and I have already upgraded to the next step ahahah

Ok I'll be a good boy and buy first a caulking gun and at least try syringe filters and caulking gun before the rest haha

 

[egruberman]

The pro plus WiFi I have checked reach 15psi

Really?

Huh. Mine might suddenly have to become "broken" so my spouse will let me replace it.

 

[kain]

Really?

Huh. Mine might suddenly have to become "broken" so my spouse will let me replace it.

Unfortunately, that’s what happened to my old one

 

[Bigguy81]

The pro plus WiFi I have checked reach 15psi the only one of the latest instant pot . I'm waiting to come back in stock in the Italian website

Now I'm gonna need some fucking media bottle and silicone septa cap and a vacuum pump too, shit I haven't even started and I have already upgraded to the next step ahahah

Ok I'll be a good boy and buy first a caulking gun and at least try syringe filters and caulking gun before the rest haha

Don't waste your time bro. Get straight to vacuum filtration and then as quick as you can to using a peristaltic pump

 

[Sampei]

Don't waste your time bro. Get straight to vacuum filtration and then as quick as you can to using a peristaltic pump

Bro I ain't selling shit so I'm probably gonna stick to syringe filters, for the quantity I'll brew it looks the most sensible thing to do.

 

[Bigguy81]

Bro I ain't selling shit so I'm probably gonna stick to syringe filters, for the quantity I'll brew it looks the most sensible thing to do.

Oh ok. Sorry bro...

.....

 

[Trentok061]

@Sampei what's your opinion on this PC ?

Amazon.de

It goes up to 14,5 psi do you think that is enough to sterilize?
I'm from Europe too and have the same problem like you.

 

[Sampei]

@Sampei what's your opinion on this PC ?

Amazon.de

It goes up to 14,5 psi do you think that is enough to sterilize?
I'm from Europe too and have the same problem like you.

how much is it? Instapot can get to 15psi.
14.5psi btw is probably ok it just takes a bit longer.