@ThinkTrenbolone what temp do you have your oven on when you throw the vials in with the autoclave bags and for how long bro?
MESO-Rx
Anabolic Steroids
Pressure cooker for steriliIzing
- Thread starter Sampei
- Start date
Just 50c is more then enough it's only to dry the humidity left
Awesome thanks bro
egruberman
Well-known Member
Looks.... Okay. I'd be concerned about that valve. If it fails, there are no parts.
Most of the failures of those things are from the fact that one cooks meat or beans or whatever in them and food matter gets stuck in the valve, so maybe fine for your case.
Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications
The sterilization processes of nanoparticles (NP) by autoclaving and filtration are two of the most utilized methods in the pharmaceutical industry but are not always a viable option. For this reason, the search for alternative options such as UV and ...
www.ncbi.nlm.nih.gov
It talks about UVC light for sterilzing.
ULTRAFIALOVÁ GERMICÍDNA STERILIZAČNÁ LAMPA 32W UV-C AIR SANITIZER LEDVANCE - ledziarovka.eu
ULTRAFIALOVÁ GERMICÍDNA STERILIZAČNÁ LAMPA 32W vhodná pre alergikov
www.ledziarovka.eu
Could make a box for it and put all the vials etc in it to have concentrated UVC exposure. Not sure how much heat it produces so It might be wise to monitor temp with such a small space.
After covid I bought 2 of this in a supermarket. Original price was 60 euro, and it was off 90%, so got it for 6 eur lol.
What if no pressure cooker, but:
1. standard rinsing (dishsoap + water, then isopropyl bath, rinsing with sterile water)
2. UVC light with disassembled vials+ stoppers (60mins)
3.
4. Philips Baby bottle sterilizer with drying function, maybe 2 rounds (1 round is 45 mins with drying)
5. another round of UVC light this time with assembled vials, so no more contaminant can get in, just the light.
A) is it a viable option having no pressure cooker that is capable 15psi+?
B) what about sterilizing the finished product for less time with the UVC light? Does it harm the standard test C brew for example?
narta
Member
Most commercial uvc lights don't penetrate regular glass.
The brand new toaster is a great idea. I could buy one and send it back to Amazon every few months and exchange a new one. I have access to a fancy autoclave at a research center but it still seems to leave some question of cleanliness because I lack full control of the autoclave and it’s immediate surroundings. Also even after it’s finished with the drying cycle there is some residual moisture trapped inside of the vials so I have to transfer to a secondary oven to get it completely dry. The glass looks shiny tho.
That’s a lot of steps! My QM used to tell me cleanliness is godliness!
HB_22
Well-known Member
The instant pot, the pressure cooker that tested the best in the linked study, is widely available even in the EU. You can buy it on Amazon for 150-190€.
I must have missed it, can you link to me the model? thanks man!
bighunanballs
Well-known Member
Yeah just get an instapot if that's available in your country it's got the heater mechanism built into it It doesn't sit on the stove top
HB_22
Well-known Member
Here it is available in Germany, but also in other countries from Amazon. If you wait for "Black Friday" deal or any other special promo it is available for 120-130€.
It even has the "sterilize" function as a menu point.
The pro plus WiFi I have checked reach 15psi the only one of the latest instant pot . I'm waiting to come back in stock in the Italian website
Now I'm gonna need some fucking media bottle and silicone septa cap and a vacuum pump too, shit I haven't even started and I have already upgraded to the next step ahahah
Ok I'll be a good boy and buy first a caulking gun and at least try syringe filters and caulking gun before the rest haha
egruberman
Well-known Member
Really?
Huh. Mine might suddenly have to become "broken" so my spouse will let me replace it.
Unfortunately, that’s what happened to my old one
Don't waste your time bro. Get straight to vacuum filtration and then as quick as you can to using a peristaltic pumpThe pro plus WiFi I have checked reach 15psi the only one of the latest instant pot . I'm waiting to come back in stock in the Italian website
Now I'm gonna need some fucking media bottle and silicone septa cap and a vacuum pump too, shit I haven't even started and I have already upgraded to the next step ahahah
Ok I'll be a good boy and buy first a caulking gun and at least try syringe filters and caulking gun before the rest haha
Bro I ain't selling shit so I'm probably gonna stick to syringe filters, for the quantity I'll brew it looks the most sensible thing to do.
Trentok061
New Member
@Sampei what's your opinion on this PC ?
https://amzn.eu/d/hZ8RGnJ
It goes up to 14,5 psi do you think that is enough to sterilize?
I'm from Europe too and have the same problem like you.
https://amzn.eu/d/hZ8RGnJ
It goes up to 14,5 psi do you think that is enough to sterilize?
I'm from Europe too and have the same problem like you.
how much is it? Instapot can get to 15psi.
14.5psi btw is probably ok it just takes a bit longer.
