Qingdao Sigma Chemical Co., Ltd (International, US, EU, Canada and Australia domestic

Why even post comments like this. The guy has a valid question and you are just being an asshole.

It’s a rhetorical question, so don’t bother trying to come up with some witty comment in an attempt to insult me. It won’t be read
Cause if one can, for a fact, see aggregated proteins with the naked eye, that would put him up for the 8th wonder of the world
 
Cause if one can, for a fact, see aggregated proteins with the naked eye, that would put him up for the 8th wonder of the world

Not that I'm rushing to that clown's defense, but fyi:

You can see individual aggregated proteins, at least once they're larger than 100um.

IMG_0074.webp

Below that size, if there are a lot, the liquid looks cloudy.

That's why they're broken down into sub-visible (below 100um) and visible aggregate sizes (above 100um).


This shows the aggregates formed after dropping a reconstituted peptide vial, and how polysorbate as an excipient prevents aggregation:

IMG_0075.webp
 
Last edited:
Why even post comments like this. The guy has a valid question and you are just being an asshole.

It’s a rhetorical question, so don’t bother trying to come up with some witty comment in an attempt to insult me. It won’t be read
Moto,

It's not about insults... don't take it that way.

Sometimes, you just have to wait and see how a thread develops, how questions are answered. Some of these guys have profoundly refined knowledge about these subjects, and each share in their own unique way.

A King Fu master will kick his student in the chest, just to teach him the important lesson of keeping his guard up.

Some of these guys are masters at this, and at times, need to teach important lessons.

I'll bet none of us who just read that string of posts is ever going to forget the lesson on spotting aggregates... we all just got kicked in the chest!
 
Back
Top