QUANTITATIVE, DIY Elisa kits will revolutionize Steroid testing forever!

[master.on]

Among others:
Trenbolone ELISA kit
http://www.randoxfood.com/trenbolone-elisa
http://www.genemedsyn.com/ccpc1110-12115-trenbolone-elisa-kit--96-tests-28for-animal-tissue-de-100170.htm (Trenbolone ELISA kit, 96 tests (For Animal Tissue, Aquatic, Urine)-Genemed Synthesis Inc.)

They are designed to QUANTIFY Steroids in meat, shrimp, blood, urine etc.
You'll need to read the specs or ask the manufacturer about the detection range and dilute your steroid samples accordingly.
They will be highly diluted, for example the detection range for this one is 0.2-16 ng/ml with a sensivity of 0.083 ng/ml
https://www.salimetrics.com/biomarker/testosterone
Make sure you only use the approved solvents for dilution i.e. Some need Ultra pure water, not plain water.


They use a highly specific* antigen for the steroid in question.
After closely following the steps in the instructions they give a colour intensity proportional to the amount of steroid present.

These are complete kits, truly DIY
but you'd still need to outsource the colour reading, to be accurate you need a machine.
Watch

As you just saw, the lab creates a chart with a high-purity standard as reference. They don't need to be specially prepared, certified reference standards as in HPLC just high-purity TRUE PHARMACOPEA grade powders.
Make sure the lab machine can handle the same 96 well plates, and they have a filter for the specific light required to read the plate. Usually specified in nm.

They typically cost about $400 a kit but they typically contain enough chemicals for 96 samples.
So I'd use a single row of 12:
1 100% reference
2 90% ref
3 80% ref
4 70 % ref
5 60% ref
6 50% ref
7 40% ref
8 30% ref
9 20% ref
10 0 % ref
11 UG sample to test
12 duplicate of UG sample for redundancy

So you can test 8 UG samples for about $400 plus the lab charge for colorimetric analysis.
Not bad at all!
Remember these are professionally made kits, specifically designed for steroids.
(now watch bogus HPLC shills with made up charts begin to rant)

I think fellow members at Meso or AnaboliLab can get the 8 samples and some donation for it together.

 

[master.on]

This is just a general Elisa overview.
By the way Elisa stands for Enzyme Linked Immunosorbent Assay


Some of the kits are even easier, two steps in one, and even pre-coated plates.
Always follow the manufacturer instructions carefully. Ask when in doubt.

NEVER EVER mention bodybuilding.
If asked why do you buy these kits or why do you need the lab colour analysis, say something like:
-I resell meat from meat, chicken or shrimp farms and supermarkets turn down hormonized meat.
-I own an organic food restaurant and want to make sure no hormones in meat.
- I work at a meat consumer advocacy group.

When sending plates to the lab
DO NOT look like a bodybuilder. Wear formal, full or baggy clothes.
By the way these kits specify the maximum time allowed after completion (pouring chemicals, washing) before colorimetric analysis. Make sure it is a quick drive to the lab.

 

[master.on]

This time I'm going to make many small posts as to create some page breaks as to avoid crashing from too many pictures in a single page.
The images are badly needed to make it more understandable.

@Millard Baker
Can you please post some other ways to avoid image-loaded pages from crashing?
Thanks and pardon my ignorance.

 

[master.on]

Steroid reference standards have been troublesome to get as they are still controlled substances.

Many ELISA (Enzyme-LInked-Immuno-asSAy) test kits
already contain very diluted reference standards
kits seem to be available for purchase without a DEA license as their standards are too diluted (ppp=parts per BILLION, or NANOgrams per mL), and diluted in very poisonous chemicals, thus no potential for abuse.

So, first check the ELISA test kit to make sure it already includes reference standards

As an example check these tren test kits specs


1

https://www.genwaybio.com/media/custom/upload/File-1313531067.pdf
Page 2



2

https://www.4adi.com/objects/catalog/product/extras/DE-100170-Trenbolone-ELISA-kit-manual.pdf
Page 2

 

[master.on]

Then you should check out the specs for cross-reactivity

1

2

Pardon the huge size I don't know WTF

Point is
The in the first test Nandrolone only has a 4.5% cross-reactivity, compared to 41% for the second test.
Thus the vial/powder may actually contain Nandrolone and you could fall for it believing it was Trenbolone.

 

[master.on]

Next you'll need a good online calculator with units conversion
like this one
Dilution Calculator - Mass per Volume - PhysiologyWeb

 

[master.on]

If we were to dilute 1 mL of oil containing a steroid at 250 mg/mL
to 2.5 ppb = 2.5 NANOgrams/mL, so we can use the standarized solutions for comparison
(remember 1 ppb = 1 ng/mL)

If you tried to dilute it in a single step
for the mL of oil you'd need

100,000 Liters of solvent!
That's ungodly even if the solvent were plain tap water or cheap ocean water

Of course there's a way around it...

 

[master.on]

It's called serial dilution
you take a concentrated solution, dilute it, take a small portion of the previously diluted solution, dilute it again, repeat as desired

 

[master.on]

The first test kit contained standard solutions containing
0ppb, 0.1ppb, 0.3ppb, 0.9ppb, 2.7ppb and 8.1ppb

For oils testing, you should never take the top solution as 100%, to allow for possible overdosed oils.
It is also advisable to use a similar number range, i.e. for 250 mg/mL oils, might have been overdosed to 270 mg/mL

If 2.7 ppb = 100%
0 ppb = 0%
0.1 ppb = 3.70%
0.3 ppb = 11.11%
2.7 ppb = 33.33%

If possible, I'd recommend diluting the standards to have close reference ranges, especially in the upper end.
Something like 100%, 90%, 80% and so on
If so desired, the close ranges don't need to go all the way down, i.e. for us anything at or below 50% = crap.