Save your unusable raws Guaranteed.

[Thorshammer]

Nothing wrong with questioning either.

There's a lot of unknowns in this space

Another reason being able to cross check results myself and understanding the science is so important or this is all blind faith.

 

[Walter Hype]

Another reason being able to cross check results myself and understanding the science is so important or this is all blind faith.

Good standards for yourself too. Really makes me wanna grab a machine and get to work. I'll make a few more sales and buy one. Sounds fun as fuck and another hobby I can lose myself into

 

[Thorshammer]

Nothing wrong with questioning either.

There's a lot of unknowns in this space

Another reason being able to cross check results myself and understanding the science is so important or this is all blind faith

Good standards for yourself too. Really makes me wanna grab a machine and get to work. I'll make a few more sales and buy one. Sounds fun as fuck and another hobby I can lose myself into

Start studying theory and technique now while you wait thats the big part. Getting the tech is the easy part.

 

[Walter Hype]

Another reason being able to cross check results myself and understanding the science is so important or this is all blind faith

Start studying theory and technique now while you wait thats the big part. Getting the tech is the easy part.

more people need to LOVE what they do with the full intensity life gives it. to LOVE what you do means you want to be lost in it. i dont think alot who do this love it. its just job. my moto is you either love what you do or just find a new job where you do love it. you wont last unless its your life and you love it. you gotta care about the tiny details and only someone whos obsessed and loves it will care about the tiny details .

 

[Motojager]

Let's not kid ourselves here. UV Vis is awesome for seeing did my cleanup work and tracking improvement. It is fast and cheap feedback. But for knowing the real purity percentage and what’s actually in there, you still need proper lab testing like Janoshik. One is a useful check, the other is the actual answer. UV misses impurities like heavy metals, leftover solvents non UV absorbing byproducts, inorganic salts etc. There are a few thing that it is blind to. Yes recrystallization removes a lot of them (especially solvents and salts if done right), but not always 100%, and UV won't let you know either way. That said you can get a good quality UV viz for really cheap.I got my Beckman DU-530 for $600 or $700(not anything fancy like Thors but no external software needed graphs right on screen). But as said by Thor you need to be on point with your repeatability or just pissing into the wind. To be honest a good MP can be good enough for tracking progress and being confident in your process before sending out to lab.

 

[Thorshammer]

Let's not kid ourselves here. UV Vis is awesome for seeing did my cleanup work and tracking improvement. It is fast and cheap feedback. But for knowing the real purity percentage and what’s actually in there, you still need proper lab testing like Janoshik. One is a useful check, the other is the actual answer. UV misses impurities like heavy metals, leftover solvents non UV absorbing byproducts, inorganic salts etc. There are a few thing that it is blind to. Yes recrystallization removes a lot of them (especially solvents and salts if done right), but not always 100%, and UV won't let you know either way. That said you can get a good quality UV viz for really cheap.I got my Beckman DU-530 for $600 or $700(not anything fancy like Thors but no external software needed graphs right on screen). But as said by Thor you need to be on point with your repeatability or just pissing into the wind. To be honest a good MP can be good enough for tracking progress and being confident in your process before sending out to lab.

You can tell exact quantity of the substance your measuring you cant tell what the impurity is or exact ID of substance for example all esters of testosterone with be 240nm. But you can definitely find the purity of a single substance. You can not test for heavy metals with HPLC you need ICP MS and most labs dont have it. I use outside labs for my 3rd party coas and finished products because thats multiple substances and you can build a matrix blank minus solute but thats to much. But im not sending samples in between purification and I also like being able to compare those results not just have to take their word for it..

 

[Thorshammer]

I use MP more for substance ID because the MP varies more between and I have RF measurements stored for each thing also for TLC. Multiple methods. I will always use outside lab for verification but I do not want to have to trust them 100%

 

[Walter Hype]

I use MP more for substance ID because the MP varies more between and I have RF measurements stored for each thing also for TLC. Multiple methods. I will always use outside lab for verification but I do not want to have to trust them 100%

melting point is great but the thing you use to melt it needs to be pretty spot on! unironically a modified 3d heater bed stolen from my ender 3 is pretty dang spot on after PID calibration. obviously apparatus is made for that purpose but i love a good MacGyver solution. you can watch how the more pure e becomes the more stable it gets at room temp! after getting many batches iv found most wanna melt as soon as you touch it but some freshly recrystallized e that is pretty stable will sit on your hand for a bit before it just turns into a mush

 

[Motojager]

But you can definitely find the purity of a single substance.

We can get close but not to true purity if there are things in there it can't see is all I am saying. I think it is a great fucking tool. But 3rd party testing is still a validation I need. Even if I could afford the equipment or had the knowledge to do full spectrum testing I still need to rely on someone else.

 

[Walter Hype]

You can tell exact quantity of the substance your measuring you cant tell what the impurity is or exact ID of substance for example all esters of testosterone with be 240nm. But you can definitely find the purity of a single substance. You can not test for heavy metals with HPLC you need ICP MS and most labs dont have it. I use outside labs for my 3rd party coas and finished products because thats multiple substances and you can build a matrix blank minus solute but thats to much. But im not sending samples in between purification and I also like being able to compare those results not just have to take their word for it..

also running a few runs off the same sample would increase accuracy and you avg out that result and combine with many methods to get a really great spot on guess. theres some open source software that lets you use a camera and uv and ai that will run the plate for you and get pretty qualitive based on the paper i read. not perfect but its pretty close for something within 10%!~ would rule out the dif between 80 and 90% at least.

 

[Motojager]

also running a few runs off the same sample would increase accuracy and you avg out that result and combine with many methods to get a really great spot on guess. theres some open source software that lets you use a camera and uv and ai that will run the plate for you and get pretty qualitive based on the paper i read. not perfect but its pretty close for something within 10%!~ would rule out the dif between 80 and 90% at least.

With a known standard you can get really close. You don't need AI or any special software. It is a simple peak/shape you are trying to match. Where its quick and easy is crude sample to recyrst sample. Just watching the peak and shape improve. But repeatability on sample prep is key. Weighing, volume, solvent, dissolution, cuvette, concentration matching etc. MP can show you alot as well. As you get higher in zone and crisp on the melt. MP apparatus was one of my best investments. I decent one makes things alot easier on you.

 

[Thorshammer]

We can get close but not to true purity if there are things in there it can't see is all I am saying. I think it is a great fucking tool. But 3rd party testing is still a validation I need. Even if I could afford the equipment or had the knowledge to do full spectrum testing I still need to rely on someone else.

also running a few runs off the same sample would increase accuracy and you avg out that result and combine with many methods to get a really great spot on guess. theres some open source software that lets you use a camera and uv and ai that will run the plate for you and get pretty qualitive based on the paper i read. not perfect but its pretty close for something within 10%!~ would rule out the dif between 80 and 90% at least.

It's simpler than that and don't use AI for those calculations chatGPT sucks with numbers horribly and will gaslight the shit out of you. When you have a 99% sample make 6 dilutions about 10% change in concentration and plot a calibration curve then you dont even need the beer lambert math. Just see where lambda max intercects on the line graph .

 

[Thorshammer]

Your measurements are the fundamental error point. I recommend sitting in front of your balance with pipettes and get really good at accurate pipettimg because if you cant make consistent dilutions the numbers dont mean shit.

 

[Motojager]

It's simpler than that and don't use AI for those calculations chatGPT sucks with numbers horribly and will gaslight the shit out of you. When you have a 99% sample make 6 dilutions about 10% change in concentration and plot a calibration curve then you dont even need the beer lambert math. Just see where lambda max intercects on the line graph .

Its crazy to see them line up, or not when something is fucked up. I use for Test .010-.050mg/ml(a little lower on tren) you probablly using .005-.025? I have 5mm cuvettes though.

 

[Thorshammer]

Its crazy to see them line up, or not when something is fucked up. I use .010-.050 you probablly using .005-.025? I have 5mm cuvettes though.

for steroids except tren. yeah I have the 1cm path length. Other stuff depends on where I can get AU around 1. Bro I justify the equipment all kinds of ways but if I'm being honest I'm kind of a nerd and I like playing in the lab more than working.

 

[Walter Hype]

for steroids except tren. yeah I have the 1cm path length. Other stuff depends on where I can get AU around 1. Bro I justify the equipment all kinds of ways but if I'm being honest I'm kind of a nerd and I like playing in the lab more than working.

The playing is the testing and the experiments lol its all I research my brain often feels too full with info. But there's always more to learn

 

[Motojager]

for steroids except tren. yeah I have the 1cm path length. Other stuff depends on where I can get AU around 1. Bro I justify the equipment all kinds of ways but if I'm being honest I'm kind of a nerd and I like playing in the lab more than working.

It definitely has its ups and downs. It can be very satisfying and very breaking on your soul at the same time. I do find myself dorking out on small things like sterility/endo testing etc.

 

[Walter Hype]

It definitely has its ups and downs. It can be very satisfying and very breaking on your soul at the same time. I do find myself dorking out on small things like sterility/endo testing etc.

There's a service I found that will validate any of your methods with strips. Many versions. Ozone chamber is one thing im working on. Very cutting edge stuff. Zero heat needed. Pure ozone and hydrogen peroxide vapor. Very quick. Total nuke for any low temperature material. Just not compatible with most plastics except PP and PTFE. But are we really using anything else? It also destroys endos unlike autoclave. Prob the best idea I had so far and I just do it to overkill my process and I still autoclave anyways.

Glass and stainless steel is fine too. Just not 306 stainless. Has to be 316

I pump ozone into my stainless steel filter setup in a vacuum chamber and let it sit for 15 mins at lethal concentration then I autoclave it. On paper this is sufficient for 6 log reduction in endotoxins on surfaces and poreorus material. Vacuum does the heavy lifting for getting ozone where you want. 10 grams per hour in 40 L container is 10 x the amount needed compared to a commercial machine.

The only side effect is venting ozone out of your environment....makes you very sick if you don't vent...... During one of my experiments of using pure oxygen I almost blew myself up with a fire and ozone. It's ok now though I figured out what I did wrong. ...........lol .........

 

[Motojager]

There's a service I found that will validate any of your methods with strips. Many versions. Ozone chamber is one thing im working on. Very cutting edge stuff. Zero heat needed. Pure ozone and hydrogen peroxide vapor. Very quick. Total nuke for any low temperature material. Just not compatible with most plastics except PP and PTFE. But are we really using anything else? It also destroys endos unlike autoclave. Prob the best idea I had so far and I just do it to overkill my process and I still autoclave anyways.

Glass and stainless steel is fine too. Just not 306 stainless. Has to be 31

I pump ozone into my stainless steel filter setup in a vacuum chamber and let it sit for 15 mins at leath concentration then I autoclave it. On paper this is sufficient for 6 log reduction in endotoxins on surfaces and poreorus material. Vacuum does the heavy lifting for getting ozone where you want.

I do membrane testing with FTM and TSB. Use PTS for the endo. Used to do LAL reagent in he uv before I got the PTS. I had to waste so much so I got the PTS.

 

[Thorshammer]

I do membrane testing with FTM and TSB. Use PTS for the endo. Used to do LAL reagent in he uv before I got the PTS. I had to waste so much so I got the PTS.

Where you getting your PTS cartridges is it the Charles Daily. I only have like 30 cartridges left so I mainly use the LAL gel clot kits from firegene but they are 10 tests at once and it gets expensive and not quantive results. You doing manual membrane filtration or using steritest kits. I use the Toone ones and found a use milipore equinox for only 500 bucks used. I need more of those PTS cartridges.