Sterilizing Vials

[Leancuisine]

When I bought my lot of vials, I was under the belief that they were sterile, as it said so in the description. Lo and behold, they were not.
Additionally I did some more filtering through a .22 and took all my test from the other 50ml vials they were in and condensed them into the one, making 35ml of Test C at 350mg/ml.
So now I have 2 - 50ml vials that I would like to reuse as well. Also the rubber stopper looks like a $20 lot lizard on pay day.

So first I removed the cap. Don't buy an expensive decrimping tool or whatever they have. I used my leatherman and cavemaned it off.
I used the wire cutter portion of the pliers to cut a slit in the edge of the vial cap and peeled it off. Pretty simple.



Once I'm here, I rinsed them in Isopropyl Alcohol 97%, as well as the stoppers and caps.
While the vials were drying I was cutting small square pieces of tinfoil out, dipping them in benzyl alcohol, and then wrapping the tinfoil around the top of the vial.
I then laid tinfoil down in a glass brownie pan (8x8) and baked them in the oven for an hour at 350F.
For the last few minutes before the hour mark I took some rubber stoppers and caps and rinsed them with BA and placed them on sterile wax paper.
When they were done, I pulled out 2 vials and filtered 16ml of warm Test C that was at 440mg/ml. I then pushed 1 ml of BA and 3ml of BB through the same filter so I now have 20ml in the vial doses at ~352mg/ml.
As I pulled the needle out of the foil, I would unravel the foil, stick the stopper in, place the cap on top, and crimp it.
Boom. 2 vials of 20ml test.





As long as the tinfoil stays in tact, the vials should remain sterile. Personally I would not let them sit this way for more than a few hours though. I will finish sterilizing the rest of the vials I need as I brew more. Tinfoil is too thin and week to trust for long in my opinion.

I didn't see a thread about this anywhere on Meso in the past few years, so I thought I would post up what I do.

If anyone has anything better or more effective please do tell!

 

[master.on]

When I bought my lot of vials, I was under the belief that they were sterile, as it said so in the description. Lo and behold, they were not.
Additionally I did some more filtering through a .22 and took all my test from the other 50ml vials they were in and condensed them into the one, making 35ml of Test C at 350mg/ml.
So now I have 2 - 50ml vials that I would like to reuse as well. Also the rubber stopper looks like a $20 lot lizard on pay day.

So first I removed the cap. Don't buy an expensive decrimping tool or whatever they have. I used my leatherman and cavemaned it off.
I used the wire cutter portion of the pliers to cut a slit in the edge of the vial cap and peeled it off. Pretty simple.
View attachment 73969 View attachment 73970 View attachment 73971 View attachment 73972 View attachment 73973


Once I'm here, I rinsed them in Isopropyl Alcohol 97%, as well as the stoppers and caps.
While the vials were drying I was cutting small square pieces of tinfoil out, dipping them in benzyl alcohol, and then wrapping the tinfoil around the top of the vial.
I then laid tinfoil down in a glass brownie pan (8x8) and baked them in the oven for an hour at 350F.
For the last few minutes before the hour mark I took some rubber stoppers and caps and rinsed them with BA and placed them on sterile wax paper.
When they were done, I pulled out 2 vials and filtered 16ml of warm Test C that was at 440mg/ml. I then pushed 1 ml of BA and 3ml of BB through the same filter so I now have 20ml in the vial doses at ~352mg/ml.
As I pulled the needle out of the foil, I would unravel the foil, stick the stopper in, place the cap on top, and crimp it.
Boom. 2 vials of 20ml test.

View attachment 73975

View attachment 73976

As long as the tinfoil stays in tact, the vials should remain sterile. Personally I would not let them sit this way for more than a few hours though. I will finish sterilizing the rest of the vials I need as I brew more. Tinfoil is too thin and week to trust for long in my opinion.

I didn't see a thread about this anywhere on Meso in the past few years, so I thought I would post up what I do.

If anyone has anything better or more effective please do tell!

Autoclave

it ain't just heat what kills bacteria, it's mostly steam contact and pressure


Properly autoclaved vials cost $1.5-2 on Ebay/Amazon why risk your health and waste your time to save $3/hour?

 

[daylight driller]

@Leancuisine thank you, very informative. @master.on is right about the inexpensive autoclave vials on ebay $100 for 100 vials shipped is cheap. Although @master.on look up depyrogenate if done correctly dry heat will work, you can use an alcohol bath or even a caustic solution in order to depyrogenate vials. Sterilizing with dry heat can be accomplished as well its all about time and temperature.

 

[daylight driller]

@Leancuisine noticed you said you cut it with ba/bb what is your ba/bb percentage now? And how did you get cyp to hold at 440mg

 

[Docd187123]

@Leancuisine thank you, very informative. @master.on is right about the inexpensive autoclave vials on ebay $100 for 100 vials shipped is cheap. Although @master.on look up depyrogenate if done correctly dry heat will work, you can use an alcohol bath or even a caustic solution in order to depyrogenate vials. Sterilizing with dry heat can be accomplished as well its all about time and temperature.

Masteron is not correct. Dry heat is the preferred method of sterilization and depyrogenation for glassware. Autoclave is less effective.

500deg for an hour is good

 

[Docd187123]

When I bought my lot of vials, I was under the belief that they were sterile, as it said so in the description. Lo and behold, they were not.
Additionally I did some more filtering through a .22 and took all my test from the other 50ml vials they were in and condensed them into the one, making 35ml of Test C at 350mg/ml.
So now I have 2 - 50ml vials that I would like to reuse as well. Also the rubber stopper looks like a $20 lot lizard on pay day.

So first I removed the cap. Don't buy an expensive decrimping tool or whatever they have. I used my leatherman and cavemaned it off.
I used the wire cutter portion of the pliers to cut a slit in the edge of the vial cap and peeled it off. Pretty simple.
View attachment 73969 View attachment 73970 View attachment 73971 View attachment 73972 View attachment 73973


Once I'm here, I rinsed them in Isopropyl Alcohol 97%, as well as the stoppers and caps.
While the vials were drying I was cutting small square pieces of tinfoil out, dipping them in benzyl alcohol, and then wrapping the tinfoil around the top of the vial.
I then laid tinfoil down in a glass brownie pan (8x8) and baked them in the oven for an hour at 350F.
For the last few minutes before the hour mark I took some rubber stoppers and caps and rinsed them with BA and placed them on sterile wax paper.
When they were done, I pulled out 2 vials and filtered 16ml of warm Test C that was at 440mg/ml. I then pushed 1 ml of BA and 3ml of BB through the same filter so I now have 20ml in the vial doses at ~352mg/ml.
As I pulled the needle out of the foil, I would unravel the foil, stick the stopper in, place the cap on top, and crimp it.
Boom. 2 vials of 20ml test.

View attachment 73975

View attachment 73976

As long as the tinfoil stays in tact, the vials should remain sterile. Personally I would not let them sit this way for more than a few hours though. I will finish sterilizing the rest of the vials I need as I brew more. Tinfoil is too thin and week to trust for long in my opinion.

I didn't see a thread about this anywhere on Meso in the past few years, so I thought I would post up what I do.

If anyone has anything better or more effective please do tell!

Dipping vials in BA isn't going to do anything for sterilization. Skip it.

Start with a detergent or lab glassware cleaner and clean the vials thorough and rinse thoroughly with distilled water. Dry heat at 500deg for an hour or more.

Stoppers shouldn't be exposed to those high temps. I don't think a few minutes at 350 damaged them but I do no more than 200deg for the stoppers.

 

[Leancuisine]

@Leancuisine noticed you said you cut it with ba/bb what is your ba/bb percentage now? And how did you get cyp to hold at 440mg

2/22 with 50/50 GSO and EO. Will never do again. EO seemed very different. No pip. Was a bitch to get to stay in solution. Go with Test E instead. EO isn't needed and you can use lower BB to hold as high as 500 pretty easily.

 

[Leancuisine]

Dipping vials in BA isn't going to do anything for sterilization. Skip it.

Start with a detergent or lab glassware cleaner and clean the vials thorough and rinse thoroughly with distilled water. Dry heat at 500deg for an hour or more.

Stoppers shouldn't be exposed to those high temps. I don't think a few minutes at 350 damaged them but I do no more than 200deg for the stoppers.

I was hoping you would chime in! I've been using the 97% alcohol for vials and everything, but also Dip the stoppers and caps in BA before I crimp them. As well as the tinfoil. I'll bump the temp up and bake em longer! 500 seems excessive though. I think the glass my vials are made from have a ridiculous temperature it can withstand. Guess we'll find out.
I would put the vials in before I even turned the oven on so the vials would gradually heat up with the oven. Don't know if that makes a difference.

 

[Leancuisine]

Masteron is not correct. Dry heat is the preferred method of sterilization and depyrogenation for glassware. Autoclave is less effective.

500deg for an hour is good

Out of curiosity why 5 hundo? Everywhere I've been reading has said 340F for an hour is ay okay.
I could have missed something during my reading though. For some reason I have a Texas-sized hemorrhoid. Can't help but curse your name every time I move.

 

[daylight driller]

Masteron is not correct. Dry heat is the preferred method of sterilization and depyrogenation for glassware. Autoclave is less effective.

500deg for an hour is good

Everything Ive read says that autoclave is actually the preferred method. Also everything that Ive read on how to depyrogenate glassware says 250 celius and even less for sterilization. Although with dry heat you are suppose to do it more than just once.

 

[Docd187123]

2/22 with 50/50 GSO and EO. Will never do again. EO seemed very different. No pip. Was a bitch to get to stay in solution. Go with Test E instead. EO isn't needed and you can use lower BB to hold as high as 500 pretty easily.

1% BA is all that's needed Mr. Cuisine. I never go higher than that and parenteral drugs use 0.9%.

 

[master.on]

@Leancuisine thank you, very informative. @master.on is right about the inexpensive autoclave vials on ebay $100 for 100 vials shipped is cheap. Although @master.on look up depyrogenate if done correctly dry heat will work, you can use an alcohol bath or even a caustic solution in order to depyrogenate vials. Sterilizing with dry heat can be accomplished as well its all about time and temperature.

Masteron is not correct. Dry heat is the preferred method of sterilization and depyrogenation for glassware. Autoclave is less effective.

500deg for an hour is good

Maybe, maybe not

But sterilization is only good in an enclosed environment
Are ovens perfectly sealed to outside bacteria? They are not

Unless you're a cheapo-ass source looking to save every last dime,
Why risk your health when you can buy 10 vials for $15 or $20 on Ebay/Amazon, some sterilized at large, industrial autoclaves
the same ones big-pharma used for almost a century with zero issues?


What's next? Making your own BB from BA to save a couple dollars?
(and inject impurities yourself in the process)
Synthesis of benzyl benzoate

 

[Docd187123]

I was hoping you would chime in! I've been using the 97% alcohol for vials and everything, but also Dip the stoppers and caps in BA before I crimp them. As well as the tinfoil. I'll bump the temp up and bake em longer! 500 seems excessive though. I think the glass my vials are made from have a ridiculous temperature it can withstand. Guess we'll find out.
I would put the vials in before I even turned the oven on so the vials would gradually heat up with the oven. Don't know if that makes a difference.

Dry heat depyrogenation needs high temps or lower temps with lover time but they aren't exactly equivalent in results. Using a combo of high temps and longer time makes it a bit more efficient at killing bacteria and endotoxins. I got my goals from medlabsupply and they take 500deg all day erryday lol.

BA doesn't sterilize anything and it's very, very weak at killin any bacteria. It's use is to prevent new bacteria from growing so dipping anything in it is not doing much of anything. The bottom of the stopper will already be exposed to BA once you fill and cap the vial and that's enough. The outside of the stopper will be exposed to air and other shit so it doesn't matter what you do it it before, it will always get contaminated. Dip the stoppers in 70ish % isopropyl for about 15-20min then dry in the oven at 180-200deg for 20min or so. Use as quickly as possible.

Putting vials in oven as it heats will make slight differences but you still want the time of about an hour or more inside the oven when it reaches temps.

Edit* you can autoclave the stoppers too

 

[daylight driller]

Dry heat and an autoclave are both sufficent for sterilization. They both have advantages. If you look into scientific paper you will find this schedule for dry heat

 

[Docd187123]

Out of curiosity why 5 hundo? Everywhere I've been reading has said 340F for an hour is ay okay.
I could have missed something during my reading though. For some reason I have a Texas-sized hemorrhoid. Can't help but curse your name every time I move.

Lmao!!! Curse my name like I do yours

500deg bc dry heat oven tunnels used in pharmaceutical companies use clean and unidirectional air. Your oven has food residue and crap in it withou having constant fresh clean air being drawn over them. Also, a dry heat tunnel has multiple temp sensors to ensure its actually at required temps. Your ovens thermometer can be off an easy 10-15deg or more and there are hot and cold spots in the ovens that can alter actual temps considerably more. It's better safe than sorry IMO to use more heat and more time bc after a 3-log reduction in endotoxins, they don't die off linearly after that. It's a biphasic reduction so again, better safe than sorry.

Everything Ive read says that autoclave is actually the preferred method.

It's preferred but that depends upon the application. You cannot use wet heat sterilization for oil based compounds, water sensitive materials, etc and it's generally accepted that dry heat or terminal sterilization is much better and should be used whenever possible over wet heat/autoclave.

Also everything that Ive read on how to depyrogenate glassware says 250 celius and even less for sterilization. Although with dry heat you are suppose to do it more than just once. View attachment 74003 View attachment 74004

I'm glad you posted that bc I think it's the same reference I will talk about here. FDA stops short of recommending time and temps for dry heat tunnels. What they do state is that a 3-log reduction in endotoxin quantity must be accomplished. In that study, posted here A Comparative Study of Different Methods for Endotoxin Destruction , autoclaves were unable to achieve a 3-log reduction in endotoxin quantity. Only dry heat was able to do that.

As for why I suggest high temps and long times, see my response to LeanCusiine.

 

[Leancuisine]

Lmao!!! Curse my name like I do yours

500deg bc dry heat oven tunnels used in pharmaceutical companies use clean and unidirectional air. Your oven has food residue and crap in it withou having constant fresh clean air being drawn over them. Also, a dry heat tunnel has multiple temp sensors to ensure its actually at required temps. Your ovens thermometer can be off an easy 10-15deg or more and there are hot and cold spots in the ovens that can alter actual temps considerably more. It's better safe than sorry IMO to use more heat and more time bc after a 3-log reduction in endotoxins, they don't die off linearly after that. It's a biphasic reduction so again, better safe than sorry.




It's preferred but that depends upon the application. You cannot use wet heat sterilization for oil based compounds, water sensitive materials, etc and it's generally accepted that dry heat or terminal sterilization is much better and should be used whenever possible over wet heat/autoclave.



I'm glad you posted that bc I think it's the same reference I will talk about here. FDA stops short of recommending time and temps for dry heat tunnels. What they do state is that a 3-log reduction in endotoxin quantity must be accomplished. In that study, posted here A Comparative Study of Different Methods for Endotoxin Destruction , autoclaves were unable to achieve a 3-log reduction in endotoxin quantity. Only dry heat was able to do that.

As for why I suggest high temps and long times, see my response to LeanCusiine.

Exactly. Better safe than sorry. That's why I always buy TWO packs of babybel cheese, because I know there is a 70% chance the first pack will be gone by the time I get home.

 

[Docd187123]

Exactly. Better safe than sorry. That's why I always buy TWO packs of babybel ch**se, because I know there is a 70% chance the first pack will be gone by the time I get home.

If you hear of a hairy, fat powerlifter found hanging from a ceiling rafter without a note....you'll know who it is.

 

[Leancuisine]

Maybe, maybe not

But sterilization is only good in an enclosed environment
Are ovens perfectly sealed to outside bacteria? They are not

Unless you're a cheapo-ass source looking to save every last dime,
Why risk your health when you can buy 10 vials for $15 or $20 on Ebay/Amazon, some sterilized at large, industrial autoclaves
the same ones big-pharma used for almost a century with zero issues?


What's next? Making your own BB from BA to save a couple dollars?
(and inject impurities yourself in the process)
Synthesis of benzyl benzoate

Thought about it, but my dick juices are a lot better solvent. Not the semen. The pre cum.

 

[Leancuisine]

If you hear of a hairy, fat powerlifter found hanging from a ceiling rafter without a note....you'll know who it is.

If you leave the location of your AAS Stash to me, I'll bring a pan of brownies to your grave every week so your spirit can smell the aromas.
I think that's A thing, right?
I can pull up a chair and tell you all about my week while enjoying a grilled cheese sandwich with mozzarella sticks.

 

[master.on]

bottomline:
don't bother sterilizing any vials

if anything, just sterilize receiver bottles in vacuum filtration units.