We've all seen the posts before where someone drawing 1 ml from a 10 ml vial comes up short on the last draw.
The answer is always that some oil remains in the needle and the needle hub and that small amount adds up to be close to 1 ml by the time the vial is completely tapped.
For science (and my own curiosity) i wanted to test a theory that i had. i pulled .5 ml from a vial using an insulin syringe with a fixed 27g, .5" needle with virtually no dead space.
i pulled the plunger down further after drawing to get all the oil and to make sure i had a precise .5 ml.
i transferred the .5 ml into a BD 3 ml syringe and pulled the plunger down to the 2 mark.
Below you will see that the volume is one slash higher than it's supposed to be.
Now before someone drones on about why anyone would worry about .1 ml, save your breath. That's not the point of this post.
The point is to come to a definitive conclusion for why so many people come up short. Could it be as simple as the markings on the syringe aren't accurate?
i apologize, the pic is cropped and pretty blurry, but you can still see the lines and oil level.