AlexDavis43
Member
How would one conduct a receptor binding study or test for receptor binding? And functional assays?
It's nerd shit in cell culture studies - 30000 foot view:
The growth hormone receptor is expressed in a cell line and they see if/how the new GH compound binds (&/or competes with native GH if it's supposed to do that)
Functional assay would be measuring something that activating the receptor is supposed to do (this would be their example of a proliferation assay)
Something could bind the wrong spot and pass the binding assay but since it's the wrong spot, it wouldn't activate downstream signaling
