if you're working in a gmp workplace forget what i said, from your messages i dont assume that.
say that to guidelines everywhere and phifzers label or the many studies. atleast do 5 minute research so you dont end up giving yourself an infection is all im trying to help you with or other people thats reading your message that wanna make cheap bac water.
make 10ml vials instead of 100ml if you're not using it within a month or two
the main point he dosent get is puncturing your vial introduces contaimanation and other germs. the bac dosent kill it but can hold it back for 30 days before the big pharma studies showed low level risk of infection and other issues. its 101 basic and super cheap just throw it out after 30 days
I did not want to sound rude and I appreciate your concerns and that u want to help others not go get an infection
I don't work under GMP conditions and don't brew myself steroids, but I do have a laminar flow hood and I do have experience with controlling contaminations, since I'm into mycology and need to work under sterile conditions. Before I worked with a laminar flow hood, I rarely had contaminations with my liquid cultures and they contain 4 percent sugar, proteins and other stuff microorganisms can grow in. Just wiping everything with isopropanol and keeping everything clean worked quite well. I used some of my liquid cultures for months without a contamination. Of course rarely some contaminations happened, but it worked out quite well.
If that worked well, how should BAC water only last 30 days before the risk of contamination is too high?
I don't want to sound rude, but I'm just not sure about the rational of 30 days. The 30 day rule circulates around since years and I could not find convincing research about it.
30 days is just a nice number we can remember easily. If a month would have 40 days, we would have probably the 40 day rule. Drugs also have a expiration date, but we know that some of them are usable even decades later without much degradation.
Also one could puncture the vial 3 times over the course of 3 months or 5 times a week over 30 days. The second one would definitely have a higher risk of contamination and I would throw that vial out after some reconstitutions.
Microorganisms surely don't grow in pure bac water (there's no energy source, no nitrogen for amine synthesis etc), but they can stay dormant and contaminate future reconstitutions but the risk doesn't depend on time but the number of penetrations of the vial. So instead of talking of a 30 day rule, it would probably make more sense to talk about a X penetrations/reconstitutions rule.
Each penetration brings a risk to introduce some microbes into the BAC water.
Also 100 ml water for injection vials are easier to source for at my pharmacy than 10 ml vials. That's the main reason I use them.