Use more or less bac water for hgh?

[LeeroyMatinta]

„The higher you dilute the more chances of the peptides interacting with the ions in the bac water which could cause slight degradation to peptides. Always dilute with the least amount of bac water possible, but enough that you can draw up a measurable amount in the syringe. 2ml of bac is plenty.“

I found this on reddit, is it true?

 

[Nidus]

I would take everything posted on reddit with a giant grain of salt. I have not seen any studies or actual evidence ever about this.

 

[Ghoul]

I would take everything posted on reddit with a giant grain of salt. I have not seen any studies or actual evidence ever about this.

It's absolutely false. The higher the concentration the greater the rate of aggregation of the proteins rendering them inactive and worse.

 

[narta]

It's absolutely false. The higher the concentration the greater the rate of aggregation of the proteins rendering them inactive and worse.

So we dilute the 10iu of hgh with 10ml bac? Or there is a min amount of recommended bac like 1ml for 6mg hgh according to Serono?

 

[lilhawk]

I mix Serostim with .9ml of Bac water. that way each 10 units on insulin syringe is 2iu of GH. Been doing it for years this way and never a problem.

 

[tweek]

Found on reddit? Lol I wouldn't take anything seriously on reddit

 

[Ateam2023]

„The higher you dilute the more chances of the peptides interacting with the ions in the bac water which could cause slight degradation to peptides. Always dilute with the least amount of bac water possible, but enough that you can draw up a measurable amount in the syringe. 2ml of bac is plenty.“

I found this on reddit, is it true?

 

[birdland]

Random but what is the shelf life on bac water? Does it go bad?

 

[Juiced_lizard]

Random but what is the shelf life on bac water? Does it go bad?

No it’ll be fine for years

 

[Ghoul]

So we dilute the 10iu of hgh with 10ml bac? Or there is a min amount of recommended bac like 1ml for 6mg hgh according to Serono?

That's the highest concentration of GH I ever found in a commercial formulation. Of course that's under perfect conditions, with anti-aggregation excipients, so going a little more dilute than that with UGL is probobly a good idea, especially if it's going to take more than a few days to get through the vial. 36iu I'd use 3ml.

The other factor with dilution is the pharmacokinetics, ie, how fast or slow the drug is absorbed. Higher concentration the faster blood levels rise. But unlike other peptides like GLPs where too quick absorbtion can worsen sides or hypoglycemia, none of the pharma companies seem to care about that with GH, so I assume it doesn't matter.

You almost can't go wrong with GH as long as it's not more than about 15iu / ml. More dilution is better for long term stability (stuffing more peptide molecules in a small volume of liquid always worsens aggregation since they bump into each other).

 

[xrayphoton13]

I made two changes at the same time so I'm not sure which one it was but I switched my 36ius from 1cc to 2cc BAC water, and started to slowly let the water enter instead of firing it in and letting it foam and instantly that night my CT that I've always gotten at 6-8iu so bad that I sometimes wore wrist braces is completely gone. So I can't explain it but I've been doing it this way for over a week now and 0 signs of CT. Batch is still the same qsc I've always been using. I'm dumbfounded

 

[Ghoul]

For anyone who wants to nerd out on the subject:


Probably the easiest to read scientific paper on the topic of aggregation factors:

https://royalsocietypublishing.org/doi/10.1098/rsfs.2017.0030

And a little less easy to read:

https://www.cell.com/fulltext/S0006-3495(08)78463-7

 

[Ghoul]

I made two changes at the same time so I'm not sure which one it was but I switched my 36ius from 1cc to 2cc BAC water, and started to slowly let the water enter instead of firing it in and letting it foam and instantly that night my CT that I've always gotten at 6-8iu so bad that I sometimes wore wrist braces is completely gone. So I can't explain it but I've been doing it this way for over a week now and 0 signs of CT. Batch is still the same qsc I've always been using. I'm dumbfounded

I've heard this before, but mainly as the difference between batches, or ugl/pharma, not the reconstitution method, which is interesting.

Obviously HGH can cause CT.

So can hyped up immune system from inflammation.

So maybe the foaming reconstitution caused enough degradation (aggregate formation) that an immune reaction added to the "regular" CT effect of GH so that the combo caused acute CT, but the "clean HGH" didn't. Would've been interesting to see if filtering made a difference by removing the aggregates.

Another really far out possibility is that we know foam causes dimerization of HGH. Regular dimer is weaker HGH. But when HGH dimers cross link, which can happen in the vial, they become a super potent form of HGH, sticking to the receptors a lot more strongly, and maybe acting like a much larger dose would, worsening sides.

Cross-linked growth hormone dimers have enhanced biological activity - PubMed

In this study we have investigated the effect on the bioactivity of pituitary-derived human growth hormone (hGH) and recombinant bovine (b) GH after the addition of various concentrations of the water soluble cross-linking agent 1-ethyl-3(3-dimethylaminopropyl) carbodiimide (EDC; 6.25-100...

pubmed.ncbi.nlm.nih.gov

 

[AlexDavis43]

„The higher you dilute the more chances of the peptides interacting with the ions in the bac water which could cause slight degradation to peptides. Always dilute with the least amount of bac water possible, but enough that you can draw up a measurable amount in the syringe. 2ml of bac is plenty.“

I found this on reddit, is it true?

It's weird they give that warning then say 2 mL is plenty.

2 mL is close to the max amount that fits in a vial (3 mL).

After the warning I would've expected them to say something like 0.5 mL. But it's nonsense anyway.

 

[SBD1]

It's weird they give that warning then say 2 mL is plenty.

2 mL is close to the max amount that fits in a vial (3 mL).

After the warning I would've expected them to say something like 0.5 mL. But it's nonsense anyway.

You can fit 3.75mL+/- in a 3mL vial. 2mL is closer to halfway than max

 

[narta]

I've heard this before, but mainly as the difference between batches, or ugl/pharma, not the reconstitution method, which is interesting.

Obviously HGH can cause CT.

So can hyped up immune system from inflammation.

So maybe the foaming reconstitution caused enough degradation (aggregate formation) that an immune reaction added to the "regular" CT effect of GH so that the combo caused acute CT, but the "clean HGH" didn't. Would've been interesting to see if filtering made a difference by removing the aggregates.

Another really far out possibility is that we know foam causes dimerization of HGH. Regular dimer is weaker HGH. But when HGH dimers cross link, which can happen in the vial, they become a super potent form of HGH, sticking to the receptors a lot more strongly, and maybe acting like a much larger dose would, worsening sides.

Cross-linked growth hormone dimers have enhanced biological activity - PubMed

In this study we have investigated the effect on the bioactivity of pituitary-derived human growth hormone (hGH) and recombinant bovine (b) GH after the addition of various concentrations of the water soluble cross-linking agent 1-ethyl-3(3-dimethylaminopropyl) carbodiimide (EDC; 6.25-100...

pubmed.ncbi.nlm.nih.gov

CT is linked directly to HGH edema. It's a physical condition due to pressure applied to nerve endings from the increased water retention.

Stop injecting your fear mongering bs to everything resulting in harm to the community.

This is a harm reduction forum and you are doing the harm by giving theoretical advice with zero first hand knowledge on things you don't even use

 

[rexy]

I made two changes at the same time so I'm not sure which one it was but I switched my 36ius from 1cc to 2cc BAC water, and started to slowly let the water enter instead of firing it in and letting it foam and instantly that night my CT that I've always gotten at 6-8iu so bad that I sometimes wore wrist braces is completely gone. So I can't explain it but I've been doing it this way for over a week now and 0 signs of CT. Batch is still the same qsc I've always been using. I'm dumbfounded

Interesting. I've recently had a batch of non-vacuum HGH and I've noticed no CT symptoms since switching from vacuum. I would screw up and direct the jet at the puck in the vacuum vials more often than not, but I always carefully do it drop by drop down the side when the vacuum isn't there. I didn't change volume of injection and my actual dose has gone up since - 15IU per day now vs. 10IU. I know this is breaching a controversial topic and I wouldn't therefore say non-vacuum is superior etc, but it just stopped me from loading that water like a jet stream straight into the puck more reliably, and just thought it added up with your experience.

 

[Ateam2023]

You can fit 3.75mL+/- in a 3mL vial. 2mL is closer to halfway than max

i use 2 ml per vial of hgh and definitely have ALOT more room for bac water if needed , ,