Using Peptide Raws Without Lyophilization?

[nixu]

Apologizes if my question is ignorant.

I've tried to do some research on peptide raws and what I've seen is most people emphasize on freeze-drying before using them. From what I understand, lyophilization is primarily done to improve long-term stability during storage. How critical is this step if the intention is to use the raw material relatively quickly?
I would, of course, filter through a 0.22 µm filter, use a high-quality accurate scale for dosing, check pH, and try to maintain sterility to the best of my ability.

Does anyone have experience homebrewing peptide raws without freeze-drying? Would skipping the lyophilization step be possible for GLP or BPC-157 powders?

 

[gainslol]

I've done it and it works but as for safety goes it's hard to say.

It is my understanding that lyophilization isn't only done for stability but by removing the water it also gives less room for bacterial growth.

Measuring small doses accurately can also be difficult and the powder will be exposed to air the entire time.

Milligram scales often have an error rate of 5-10mg and for some peptides that's a lot. This of course becomes less of a problem if you plan to reconstitute more at once.

 

[frenexen]

I do it with every peptide I take. I use a mg accurate scale with draft shield. This will cost at least a few hundred for an accurate one. Use a supplier that will provide water content of raw peptide so you can account in measurements.

Pre-split into small 3-5ml containers and protect from humidity. Then just add BAC and filter into sterile vial when you want to use it 1 at a time.

Freeze drying is needed to protect peptide from humidity in differing environments. Most peptides are much more stable than people think. I've used HGH raws over a year old and they still worked fine. As long as you store properly you shouldn't have any issues.

 

[lipzz17]

I do it with every peptide I take. I use a mg accurate scale with draft shield. This will cost at least a few hundred for an accurate one. Use a supplier that will provide water content of raw peptide so you can account in measurements.

Pre-split into small 3-5ml containers and protect from humidity. Then just add BAC and filter into sterile vial when you want to use it 1 at a time.

Freeze drying is needed to protect peptide from humidity in differing environments. Most peptides are much more stable than people think. I've used HGH raws over a year old and they still worked fine. As long as you store properly you shouldn't have any issues.

Do you use as soon as reconstitute ? Or can be put in fridge ? 1 mg of hgh raw is equivalent to how much of finished product ?

 

[frenexen]

Do you use as soon as reconstitute ? Or can be put in fridge ? 1 mg of hgh raw is equivalent to how much of finished product ?

You can store it in the fridge like any reconstituted peptide after filtering. HGH specifically though lacks some excipients that are commonly added to enhance stability so I’d use within a couple days unless you add your own to the raw.

1mg = 3 IU, but you need to account for any water content or additives added by your supplier of course.

 

[lipzz17]

You can store it in the fridge like any reconstituted peptide after filtering. HGH specifically though lacks some excipients that are commonly added to enhance stability so I’d use within a couple days unless you add your own to the raw.

1mg = 3 IU, but you need to account for any water content or additives added by your supplier of course.

Hmm i see, a little bit complicated haha, need a very good scale too i suppose, semi analytical can be used ?

 

[frenexen]

Hmm i see, a little bit complicated haha, need a very good scale too i suppose, semi analytical can be used ?

I’m sure as long as the brand is good. I use an ohaus spx123. Accurate and not too expensive