T-Bagger
Member
Looking for thoughts on what is better or if a UVS would be an acceptable replacement for an autoclave? People use their ovens for their vials, so would the sterilizer be any different/worse/same as an oven or autoclave?
MESO-Rx
Anabolic Steroids
UV Sterilizer vs Autoclave
- Thread starter T-Bagger
- Start date
Subbed on this one. I was looking at these earlier this week and was trying to find out just how good they are for sterilizing. I was thinking it could be used to sterilize non-sterile bottletop filters.
T-Bagger
Member
I don’t see why it couldn’t be used for all things. The pics I saw were for salon tools, so why not anything and everything else?
Same ones I was looking at. I just couldn’t find any info about the level of sterilization they are able to achieve.
T-Bagger
Member
T-Bagger
Member
Also found this article interesting. Most interesting is the part that says UV radiation is not good at killing bacteria where parts are overlapping. It would appear to me that UV sterilization of caps, stoppers and vials is more than sufficient. If you think about it, they sell UV sterilizer for baby bottles. If it’s good enough to sterilize those, surely vials and etc. are going to be sterilized as well.
https://waterpower.pnnl.gov/jsats/pdf/PNNL-21126.pdf
https://waterpower.pnnl.gov/jsats/pdf/PNNL-21126.pdf
MisterSuperGod
New Member
Where's that fucking robot to tell you all about it? He might even have a step by step guide on how to make your own with a couple lightbulbs, some duct tape and a garden hose.
i'm not tagging him. He might think that i like him.
i'm not tagging him. He might think that i like him.
T-Bagger
Member
I considered tagging himWhere's that fucking robot to tell you all about it? He might even have a step by step guide on how to make your own with a couple lightbulbs, some duct tape and a garden hose.
i'm not tagging him. He might think that i like him.
T-Bagger
Member
I found a cabinet that is being sold to dentist offices and have asked them my question regarding vials, caps and stoppers. They said they answer in 24-48 hours, so I’ll post it when they reply. Not bad for $120.
It's possible that the UV light can work on metal and not glass.
T-Bagger
Member
Idk man, they have baby bottle sterilizers and those are plastic. I would think glass is the easiest to sterilize.
T-Bagger
Member
@master.on your input would be greatly appreciated!
JackSmooth
Member
If the light can not contact it then it can not be sterilized. Like bottle top dispensers for example.
This is what I think.
T-Bagger
Member
Someone told me they use a UV sterilizer for a transfer tube. I suppose if it’s a clear tube, the light is touching it and works?
So it sounds like you’re saying the stoppers and caps would not be good candidates but the vials would be fine?
JackSmooth
Member
Correct. Now that's only an assumption, Im no expert on how they work.
Why not go clave? I got one on ebay for 300. The time and peace of mind is well worth 300.
T-Bagger
Member
I like cost efficiency. They’ve actually raised the prices on claves
boost creep
Member
I would be concerned with the inside of a vial. Glass is reflective even clear glass to an extent. JMO/IDK? lol. I do know if you heat the fuck out of something it will kill bacteria.
master.on
New Member
1 UV won't kill enough bacteria.
2 If it's meant for personal use, just buy sterile vials. i.e. Hospira is true big pharma quality and they're like $5 at Amazon or Ebay.
3 If it's meant for anything larger scale
buy a wet autoclave (those that leave vials wet after sterilization), they are cheap and widely available
then rinse with STERILE FILTERED 75% alcohol and allow them to fully dry, in a sterile enclosure.
4 Dry heat sterilization ain't suitable because vials glass ain't heat resistant and proper sterilization would need high temperatures and long times that would surely crack glass (and melt rubber stoppers).
Aluminum wrapped oven heating just doesn't work. Vials would need to be exposed to hot air (to get exposed to hot Oxygen) and that would need a huge, expensive sterile oven.
https://microbeonline.com/dry-heat-sterilization-principle-advantages-disadvantages/
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T-Bagger
Member
Hmm... this is totally contrary to what many believe of an autoclave. If you get the wet clave with heated dry you’re saying that isn’t sufficient either?
What about these UGL’s that set their oven to 500°F - is that not sufficient sterilizing?
master.on
New Member
While autoclaves don't leave vials that wet,
even a little water condensation can make oil based formulations cloudy.
Sterile filtered 70% alcohol is a great and cheap way to ensure vials are bone dry.
Baking in oven doesn't work, as ovens aren't sterile
and they usually wrap vials in Aluminum foil that prevents hot air from getting in.
