Very uneducated question about peptide raws

[Luke391]

1) Why is the raw powder lyphilized? is it for sterility or for preservation? If it is for sterility, wouldn't reconstituting the raw powder right when you have to use it and filtering it with 0.22um achive the same result?
I don't understand why it would be for preservation since the raw powder can be preserved as is, without being lyophilized.

2) How is it mixed with mannitol or other fillers without loosing a lot of the raw peptide and ensuring the peptide is evenly distributed trough the filler?

I don't plan to sell so likely will never buy raw powders but I'm curious.

 

[Dial It In]

Increases shelf life and micro bacterial growth. Microbacterial growth can degrade the product. Back in the day peptides coming constituted would lump up in the vial

 

[Luke391]

Increases shelf life and micro bacterial growth. Microbacterial growth can degrade the product. Back in the day peptides coming constituted would lump up in the vial

ah thanks. I'm aware of how dumb I sound asking this, but if I was to take the raw peptide powder, mix with mannitol and maybe a buffer like citrate to keep ph stable, and portion directly into sterile vials. Could I then when I have to use it reconstitute it and filter it to make it sterile? Or would not lyphilizing or fuck something up?
Like what if I made single dose vials and filter it vial by vial at the moment of use?

 

[BigDadd7]

ah thanks. I'm aware of how dumb I sound asking this, but if I was to take the raw peptide powder, mix with mannitol and maybe a buffer like citrate to keep ph stable, and portion directly into sterile vials. Could I then when I have to use it reconstitute it and filter it to make it sterile? Or would not lyphilizing or fuck something up?
Like what if I made single dose vials and filter it vial by vial at the moment of use?

He just gave you the answer, and it was no

 

[Luke391]

He just gave you the answer, and it was no

but with single dose vials degradation or microbacterial growth wouldn't be an issue no? the raw powder is stable as is, so why would it not be once put in a vial?

 

[Dial It In]

ah thanks. I'm aware of how dumb I sound asking this, but if I was to take the raw peptide powder, mix with mannitol and maybe a buffer like citrate to keep ph stable, and portion directly into sterile vials. Could I then when I have to use it reconstitute it and filter it to make it sterile? Or would not lyphilizing or fuck something up?
Like what if I made single dose vials and filter it vial by vial at the moment of use?

Are you wanting to add mannitol when you reconstitute? Mannitol is already added to a lot of peptides to help with that caking, some have sorbitol. Not sure I’m tracking what you are trying to do. If you are trying to lyphilize peptide then most need some sort of bulking agent like mannitol for stabilization. I’m not as familiar with peptide raws but maybe I can get someone in here to help with tons of knowledge and of the smartest fellas round here. @Sampei

 

[BigDadd7]

It's made in giant batches, scooped into containers, and then whatever small portion you purchase is scooped out, weighed, and dropped into a plastic bag. What makes you think it's still sterile at that point?

 

[Ghoul]

1) Why is the raw powder lyphilized? is it for sterility or for preservation? If it is for sterility, wouldn't reconstituting the raw powder right when you have to use it and filtering it with 0.22um achive the same result?
I don't understand why it would be for preservation since the raw powder can be preserved as is, without being lyophilized.

It's for preservation. Protein in its natural state is "wet". In pharma it's always maintained in a sterile condition. In UGL it's just handled like any other powder, and therefore unsterile.

Carefully freeze drying proteins / peptides via lyophilization preserves it that same way eggs (almost entirely protein) last much longer when dried and powdered.

2) How is it mixed with mannitol or other fillers without loosing a lot of the raw peptide and ensuring the peptide is evenly distributed trough the filler?

The raw is reconstituted using a precise amount of water, the excipients like mannitol are added to this brew, it's gently but thoroughly mixed, vials are filled using a syringe with a .22um PES filter attached, then stoppers are loosely placed in each vial, and it's run through the lyophilization machine. A manual or automatic mechanism then presses the stoppers into place while the vials are still under vacuum. Finally they're removed and collars with caps are crimped over each.

Unfortunately unlike pharma, this isn't done in a certified clean room, so while the filtering sterilizes, the vials and environment are often unsterile, leading to a high proportion of peptides being unsterile.

 

[Dial It In]

It's made in giant batches, scooped into containers, and then whatever small portion you purchase is scooped out, weighed, and dropped into a plastic bag. What makes you think it's still sterile at that point?

Oh for sure gotta run a sterilization on raws.

 

[Dial It In]

It's for preservation. Protein in its natural state is "wet".

Carefully freeze drying proteins / peptides via lyophilization preserves it that same way eggs (almost entirely protein) last much longer when dried and powdered.



The raw is reconstituted using a precise amount of water, the excipients like mannitol are added to this brew, it's gently but thoroughly mixed, vials are filled using a syringe with a .22um PES filter attached, then stoppers are loosely placed in each vial, and it's run through the lyophilization machine. A manual or automatic mechanism then presses the stoppers into place while the vials are still under vacuum. Finally they're removed and collars with caps are crimped over each.

Unfortunately unlike pharma, this isn't done in a certified clean room, so while the filtering sterilizes, the vials and environment are often unsterile, leading to a high proportion of peptides being unsterile.

Ah I knew a smart one would show up

 

[dinfar1337]

Unfortunately unlike pharma, this isn't done in a certified clean room, so while the filtering sterilizes, the vials and environment are often unsterile, leading to a high proportion of peptides being unsterile.

get a big enough flow hood to fit a lyophilizer in there, work with sterile equipment from the get go and chinese raw powders test sterile "enough".

best we can do when homebrewing peptides. no need to stress it, most likely what every ugls does and there seems to be no issues whatsoever.

its still not 100% sterile but sterile enough

 

[BigDadd7]

You just won't take no for an answer

 

[Luke391]

It's for preservation. Protein in its natural state is "wet". In pharma it's always maintained in a sterile condition. In UGL it's just handled like any other powder, and therefore unsterile.

Carefully freeze drying proteins / peptides via lyophilization preserves it that same way eggs (almost entirely protein) last much longer when dried and powdered.



The raw is reconstituted using a precise amount of water, the excipients like mannitol are added to this brew, it's gently but thoroughly mixed, vials are filled using a syringe with a .22um PES filter attached, then stoppers are loosely placed in each vial, and it's run through the lyophilization machine. A manual or automatic mechanism then presses the stoppers into place while the vials are still under vacuum. Finally they're removed and collars with caps are crimped over each.

Unfortunately unlike pharma, this isn't done in a certified clean room, so while the filtering sterilizes, the vials and environment are often unsterile, leading to a high proportion of peptides being unsterile.

what would happen if one was to use a unlyophilozed peptie? like would reconstituting a single dose and using right away avoid the degradation (obviosuly filtering)

 

[dinfar1337]

You just won't take no for an answer

i swear ive told him its too expensive to indulge in to.

ive spent 1000 buck on building my lyophilizer having to add a vacuum to it. it just fit condenser temperatures.

and now i have to spend big $$ on better gear to make more than 5 peptide vials every brew lmao

 

[Luke391]

You just won't take no for an answer

I'm trying to see if the risk exceedes my risk tolerace

 

[BigDadd7]

I'm trying to see if the risk exceedes my risk tolerace

It sounds more like you're trying to open a budget ugl. This stuff is so cheap, you could have a years worth for $300. No equipment needed and no hassle.

 

[Luke391]

It sounds more like you're trying to open a budget ugl. This stuff is so cheap, you could have a years worth for $300. No equipment needed and no hassle.

no it would just be to have a large supply for myself for a long time.

 

[BigDadd7]

no it would just be to have a large supply for myself for a long time.

Why would you need multiple years of unsterile raws that would be degrading over time because you didnt sterilize them? Make it make sense.

 

[Dial It In]

what would happen if one was to use a unlyophilozed peptie? like would reconstituting a single dose and using right away avoid the degradation (obviosuly filtering)

I mean you got to use it faster if you don’t. The longer it sits wet the faster it degrades. Mannitol doesn’t extend the shelf life of a constituted peptide the same period of time as lyophilized. Plus you still got to sterilize those raws anyway so if you going to do all that then just freeze dry it anyway.

 

[Dial It In]

no it would just be to have a large supply for myself for a long time.

Brother what peptide are you worried about That you need to stock up long term?