So a weird thing about that 97% tirz. I’ve been getting tirz for my brother since last summer, and he got to the 97% kit about a month ago. As soon as he injected it, instant ISR. Like, golf ball sized. Happened with the second shot too, so I filtered the rest of the vial for him and he finished 2 more 2.5mg shots with no issue. In the off chance that it was just that vial, he injected the first shot of a new vial (same batch) yesterday. Huge reaction again. I’ll be filtering the rest of the kit for him as he goes, but I’ll be curious if you experience the same thing. @Ghoul thoughts?
He’s also going to try tirz from a different vendor to see if he’s developed some sort of allergy to the tirz after this second vial is out.
So a weird thing about that 97% tirz. I’ve been getting tirz for my brother since last summer, and he got to the 97% kit about a month ago. As soon as he injected it, instant ISR. Like, golf ball sized. Happened with the second shot too, so I filtered the rest of the vial for him and he finished 2 more 2.5mg shots with no issue. In the off chance that it was just that vial, he injected the first shot of a new vial (same batch) yesterday. Huge reaction again. I’ll be filtering the rest of the kit for him as he goes, but I’ll be curious if you experience the same thing. @Ghoul thoughts?
He’s also going to try tirz from a different vendor to see if he’s developed some sort of allergy to the tirz after this second vial is out.
So a weird thing about that 97% tirz. I’ve been getting tirz for my brother since last summer, and he got to the 97% kit about a month ago. As soon as he injected it, instant ISR. Like, golf ball sized. Happened with the second shot too, so I filtered the rest of the vial for him and he finished 2 more 2.5mg shots with no issue. In the off chance that it was just that vial, he injected the first shot of a new vial (same batch) yesterday. Huge reaction again. I’ll be filtering the rest of the kit for him as he goes, but I’ll be curious if you experience the same thing. @Ghoul thoughts?
He’s also going to try tirz from a different vendor to see if he’s developed some sort of allergy to the tirz after this second vial is out.
Has it ever been characterized what the other 3% was? I was assuming it was other soluble peptides, if so, filtering wouldn't do anything. (as any filter that would pass tirz would also pass the impurity)
So a weird thing about that 97% tirz. I’ve been getting tirz for my brother since last summer, and he got to the 97% kit about a month ago. As soon as he injected it, instant ISR. Like, golf ball sized. Happened with the second shot too, so I filtered the rest of the vial for him and he finished 2 more 2.5mg shots with no issue. In the off chance that it was just that vial, he injected the first shot of a new vial (same batch) yesterday. Huge reaction again. I’ll be filtering the rest of the kit for him as he goes, but I’ll be curious if you experience the same thing. @Ghoul thoughts?
He’s also going to try tirz from a different vendor to see if he’s developed some sort of allergy to the tirz after this second vial is out.
It makes perfect sense that pain would be less post filtration.
Peptide aggregates, especially large aggregates trigger an immune response.
Aggregates form most easily from degraded peptide/protein. "Degraded" peptide including denatured (unfolded and not in its proper shape), peptide broken into fragments, or impurities (chains of random amino acids) left from the manufacturing process.
I've recently been reading about the way peptide aggregates tend to remain where they're injected in sub-q injection. They sit there attracting the attention of the immune system causing inflammation and pain.
Filtration eliminates most of the aggregates, and all of the large ones, reducing the injecting site reaction.
A new batch won't demonstrate that he's "allergic" to this one. If he doesn't have an ISR to the new one it just means that one isn't as damaged, and therefore there's less "peptide junk" to stick together forming aggregates.
In any case, I think you should always filter anyway. Just because things don't rise to the level of obvious symptoms like inflammation at the injection site, they can still cause numerous problems.
One other thing that reduces aggregation and site reactions is proper dilution. If you can get the Tirz dose to .5ml, or as close as possible, there will be less of a problem.
By understanding the potential causes of aggregation and its consequences, researchers can minimize the risk of immune responses and inflammation
www.pion-inc.com
"When administering drugs subcutaneously, larger particles or aggregates, can linger at the injection site for extended time periods, increasing the chances of interactions with immune cells. The human body's innate defense mechanisms can recognize these aggregates as potential threats, which can cause immune cells to engulf and destroy the drug, rendering it less effective."
The severity of an adverse immune reaction to a medication can vary, ranging from minor localized discomfort to potentially fatal anaphylaxis for the patient. Often immune reactions to a subcutaneous medicine will result in some amount of inflammation, which, if substantial, can cause significant pain for the patient."
Protein aggregates are a major risk factor for immunogenicity. Until now most studies on aggregate-driven immunogenicity have focused on linking physicochemical features of the aggregates to the formation of anti-drug antibodies. Lacking is however, ...
Has it ever been characterized what the other 3% was? I was assuming it was other soluble peptides, if so, filtering wouldn't do anything. (as any filter that would pass tirz would also pass the impurity)
The impurities easily form into aggregates, and those are effectively filtered out once they're above .2um. Since the largest aggregates cause the strongest immune reactions, filtering is an effective way to deal with them.
Aggregates form most easily from degraded peptide/protein. "Degraded" peptide including denatured (unfolded and not in its proper shape), peptide broken into fragments, or impurities (chains of random amino acids) left from the manufacturing process.
I've recently been reading about the way peptide aggregates tend to remain where they're injected in sub-q injection. The sit there attracting the attention of the immune system causing inflammation and pain.
I’ve been on the aggregate bandwagon since you’ve been beating this drum, but I gotta admit it’s a whole new thing seeing the evidence in person. His reaction definitely lasted close to 5 days, so it makes sense that the aggregates stuck around.
Man, glad I started filtering everything (in large part because of you), and I’ll teach him how to do it too.
I’ve been on the aggregate bandwagon since you’ve been beating this drum, but I gotta admit it’s a whole new thing seeing the evidence in person. His reaction definitely lasted close to 5 days, so it makes sense that the aggregates stuck around.
Man, glad I started filtering everything (in large part because of you), and I’ll teach him how to do it too.
It moved from theory to reality with me once I started using Tesamorelin, so prone to aggregation even the pharma version is known to cause painful site reactions with nearly every (daily) injection.
The first time I filtered a vial the pain disappeared, a huge eye opener. BUT, with each subsequent injection the pain progressively got worse.
Realizing aggregates must be reforming over time, I moved to the next level, loading a 3ml syringe with the entire vial contents and filtering right into an insulin syringe just before injecting.
It's how I use all my peptides now, and, in some cases, they've become so much more effective, I've had to lower the dose. I suspect it's due to less immunogenicity, so less of the drug is neutralized.
Next I plan to eliminate backfilling and the needle on the 3ml syringe by direct coupling the filter right onto a zero deadspace syringe and attach a zero deadspace 31g needle.
While searching for the CNY end date for my last post I saw this response from QSC. If anyone still thinks this was an exit scam, he did a terrible job at pulling in orders.
The impurities easily form into aggregates, and those are effectively filtered out once they're above .2um. Since the largest aggregates cause the strongest immune reactions, filtering is an effective way to deal with them.
How do you go about it just filter a vial or 2 at a time or for GH if I’m using a kit a month just reconstitute the whole thing, filter it and backload 60 slin pins
Never-mind scrolled down a few more post pics and everything.
How do you go about it just filter a vial or 2 at a time or for GH if I’m using a kit a month just reconstitute the whole thing, filter it and backload 60 slin pins
Aggregates form over time. The less time between reconstitution and use the better. Or the less time between filtering and use is better.
Since you're using a vial every 3 days, I'd reconstitute however many vials would fit into a 10ml syringe. Draw them all into the syringe, remove needle and attach filter, reattach needle.
Then backfill an insulin syringe just before you use it. Once you do it a few times it only takes seconds. Then whatever aggregates grow in syringe are filtered out and there's not enough time for new ones to form in the insulin syringe,
If that's too much work, just filter a few vials at a time (draw into syringe, attach filter, inject back into vial) and use however you normally would, or backfill a bunch of insulin syringes in advance from the filtered syringe,
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Funny how they both tested at 97%.
