10ml or 20ml or 30ml vials

[Sampei]

Sterile and Water for Injection are two different things. Sterility is not enough. Sterility only means an absence active bacteria.

Water being injected must be "pyrogen free", and sterilized distilled water isn't.

I'm sure a lot of UGL and bullshit Amazon brands of BAC isn't pyrogen free, but isn't a great idea to use it.

If you have a hard time getting pharma BAC, buy "sterile water for injection" and add benzyl alcohol to make BAC.

Well one can argue that If you buy distilled water and make it sterile (that doesn't mean pyrogenic free as you well said) with a .22um filter it should be probably very ok and not contain endotoxins at any dangerous level.

But yes it's best to use non pyrogenic water aka sterile water for injection

 

[MyNameIsJeff]

So the procedure for making BAC water would be something like this?

Step 1) Buy, say, 500ml of Sterile water and a small quantity of 99.95+% pure BA
Step 2) Add 0.908ml of BA to each 100ml of sterile water
Step 3) Swirl it around a bit (beneficial to warm it up to ~40 degree Celsius perhaps?)
Step 4) Withdraw the created BAC water with big ass syringe
Step 5) Attach a sterile 33mm hydrophilic 0.22 micron PTFE filter to the syringe, followed by a needle.
Step 6) Filter and inject the BAC water into empty, sterile, sealed vials.
Step 7) Profit

 

[Ghoul]

Well one can argue that If you buy distilled water and make it sterile (that doesn't mean pyrogenic free as you well said) with a .22um filter it should be probably very ok and not contain endotoxins at any dangerous level.

But yes it's best to use non pyrogenic water aka sterile water for injection

The main reason you want endotoxin (pyrogen) free water for peptides isn't because there will be such a high level it'll cause illness.

The immune system will respond rapidly to endotoxin injected subcutaneously, the location where the body has the strongest immune defenses, and destroy it before any symptoms develop.

But this is precisely the problem. The last thing you want to do when injecting a peptide is to draw the immune system's attention to the area, because immunogenicity to the peptide will be much worse.

Many vaccines, where you want the strongest immune system response possible, include "contaminants" specifically to draw the immune system to the area so a stronger immunity develops to the vaccine proteins being injected.

Obviously, we don't want immunity to develop to the peptides being used.

 

[bigMoJo]

i have also used a sterile .22um ptfe hydrophobic syringe filter to filter oils , i could comfortably push 30-40 ml through each filter with no problems, is there that big a difference between hydrophobic and hydrophilic when filtering oils? Or am i mistaken?View attachment 307216

Well bro, great question. Of course, @Sampei makes a great point about the condensation.

Between the two types (hydrophobic, hydrophilic):

For water based, like peptides, for sure PES hydrophilic, so the water is filtered through the filter membrane.

For AAS oils, on paper, either seems to supply the right filtering since there is no water. However, from what I gathered, hydrophobic is better for air & gases. Although, whatever small amount of water is in BA, it's brewed/mixed into the oil. Is there still any there? I don't know, I'm not a chemist. If there is, hydrophilic will filter it along with the oil, where hydrophobic will keep it out of the entire oil. Does it need to stay in with the oil? IDK. When researching, I found that the most suitable for AAS oils was hydrophilic. Now, @Sampei just put a wrench in that, lol.

To make a long post short, I think eother will do, unless there is evidence to the contrary.

 

[bigMoJo]

So the procedure for making BAC water would be something like this?

Step 1) Buy, say, 500ml of Sterile water and a small quantity of 99.95+% pure BA
Step 2) Add 0.908ml of BA to each 100ml of sterile water
Step 3) Swirl it around a bit (beneficial to warm it up to ~40 degree Celsius perhaps?)
Step 4) Withdraw the created BAC water with big ass syringe
Step 5) Attach a sterile 33mm hydrophilic 0.22 micron PTFE filter to the syringe, followed by a needle.
Step 6) Filter and inject the BAC water into empty, sterile, sealed vials.
Step 7) Profit

Jeff,
PTFE because of the alcohol, correct?

 

[Dxt25]

i have also used a sterile .22um ptfe hydrophobic syringe filter to filter oils , i could comfortably push 30-40 ml through each filter with no problems, is there that big a difference between hydrophobic and hydrophilic when filtering oils? Or am i mistaken?View attachment 307216

These are great but ebay has a brand called celltreat and feel that they are superior.