a few more GENERIC GH ASSAYS

[Dr JIM]

I dont know whats up with that lab, except the pursuit of the almighty dollar being their
priority, and that tends to limit quality IME.

Just ask them to run the results using Alanine ONLY, something they SHOULD know to do already if "matrix" interference is suspected as the cause of "innacuracies"

 

[ProfessorX]

I'm just happy I can be of help.

Regarding SIMEC, to this day I am not sure what exactly is wrong with their assays of HGH, but at least I think the consensus had been reached that those are wrong.

MANDS had mentioned that SIMEC is new to HGH testing

Now, I will agree with him about PM's HGH sample results having "issues" (not accurate)

But.....He is incorrect about SIMEC being new to testing HGH

I had "Dr A" from SIMEC do some HGH testing back in 2014 (pre-ANABOLICLAB)

Dr. A was doing testing for 'JuicedMuscle' even before that

Link:
Growth Hormone part 4 | JuicedMuscle.com

I think the issues revolve around Matthias V doing the testing instead of Dr. A, plus the volume of emails and independent samples pooring in......

This is just my opinion

There's an email I read from Matthias V expressing his frustration about this

The incredible volume of independent samples, emails, etc taking a toll on him

 

[janoshik]

Simec is not new to that, I agree.

We've been looking and found a trail of samples that had shown a trend of being 'overdosed' by their results spanning long time back.

Mr. V. certainly did not leave a good impression on me when we dealt with him, but even going through his notes I was not able to determine where the problem could've been. Might be something as stupid as using a wrong pipette tip when working with standard in the end... And there's no real way to track that.

 

[Dr JIM]

MANDS had mentioned that SIMEC is new to HGH testing

Now, I will agree with him about PM's HGH sample results having "issues" (not accurate)

But.....He is incorrect about SIMEC being new to testing HGH

I had "Dr A" from SIMEC do some HGH testing back in 2014 (pre-ANABOLICLAB)

Dr. A was doing testing for 'JuicedMuscle' even before that

Link:
Growth Hormone part 4 | JuicedMuscle.com

I think the issues revolve around Matthias V doing the testing instead of Dr. A, plus the volume of emails and independent samples pooring in......

This is just my opinion

There's an email I read from Matthias V expressing his frustration about this

The incredible volume of independent samples, emails, etc taking a toll on him

SIMEC GH "purity" results were posted on PM that used ELISA, nuf said IMO.

Moreover reading Bio Syns web site one would be led to believe they can also produce reliable results, and in some instances I suspect they can, but CONSISTENTLY ACCURATE results is the analytical gold standard!

And to that end I've every reason to believe SIMEC GH assays are INACCURATE and that was MANDS point of emphasis.

 

[ProfessorX]

SIMEC GH "purity" results were posted on PM that used ELISA, nuf said IMO.

Moreover reading Bio Syns web site one would be led to believe they can also produce reliable results, and in some instances I suspect they can, but CONSISTENTLY ACCURATE results is the analytical gold standard!

And to that end I've every reason to believe SIMEC GH assays are INACCURATE and that was MANDS point of emphasis.

SIMEC tested according to:

The European Pharmacopoeia (Pharmacopoeia Europaea, Ph. Eur.)

ASSAY : mg/vial (HPLC (EP) Reference Standard / Sigma-Aldrich)

RELATED PROTEINS :

Testing method: HPLC (To assay the percentage of rhGH and the impure proteins in the product)

DIMMER AND RELATED SUBSTANCES OF HIGHER MOLECULAR MASS TEST :

Testing method: HPLC (To assay the percentage of active protein and the impure proteins in the product)


HAIZYIUAN HYGENE BIOPHARMA

Sample F (Jim's Lab AAA)

2.46 mg/vial

SIMEC - HPLC

2.87 mg/vial

----------------

GENLEI JINTROPIN

Sample G (Jim's Lab AAA)

2.80 mg/vial

SIMEC - HPLC

2.69 mg/vial

The SIMEC testing was done by Dr A (results seem close to AAA results) compared to the recent testing done by Matthias V (posted on PM)

I'm not in disagreement with Mands about the recent GH inaccuracies from SIMEC

 

[PrideofNYC123]

SIMEC GH "purity" results were posted on PM that used ELISA, nuf said IMO.

That's not correct. There was immunoassay testing done but not via simec. Doesn't make their results anymore accurate, but wanted to correct that.


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[janoshik]

SIMEC GH "purity" results were posted on PM that used ELISA, nuf said IMO.

Are you retarded or do you lack the basic ability of comprehension?

I'm thinking both if you make such a statement that is completely wrong and has no basis on truth. I'm thinking evidence based blah blah stuff you pull out of your ass on every possible occasion is only a phrase to you.

SIMEC did standard testing with use of HPLC, Eu. Ph. methods.

I did ELISA + HPLC. Purity readings posted on PM were from the HPLC. Quantitative data were posted from both ELISA and HPLC of my doing. You cannot get purity from ELISA and I have stated many times myself it's impossible.

Clear enough for you JIM, or do we go need to go through it again?

Why do people listen to somebody who lacks any background in analytical chemistry (while refusing to educate himself, contrary to many other people who lack the background), contradicts himself half the time and lack the ability to comprehend basic information, which was even SPOONFED to him before?



Edit: Mr. Noswttea4u did this in much more polite manner, as I should probably have. But I think my anger at JIM LYING ALL AROUND is more than justified.

 

[Dr JIM]

That's not correct. There was immunoassay testing done but not via simec. Doesn't make their results anymore accurate, but wanted to correct that.


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Fair enough, bc the lack of transparency on PM creates a huge problem for anyone attempting to validate their testing, including me.

Nonetheless its my understanding SIMEC no longer conducts GH testing.

 

[muscle96ss]

Fair enough, bc the lack of transparency on PM creates a huge problem for anyone attempting to validate their testing, including me.

Nonetheless its my understanding SIMEC no longer conducts GH testing.

There is no lack of transparency whatsoever in regards to the testing done on PM as Jano just explained perfectly. Why Jim has to continue to make false statements(that have been proven to be false) suggests either a lack of understanding of simple concepts on his part or an outright purposeful attempt to lie and cover up for his third failed endeavor at GH testing.

 

[Dr JIM]

SIMEC tested according to:

The European Pharmacopoeia (Pharmacopoeia Europaea, Ph. Eur.)

ASSAY : mg/vial (HPLC (EP) Reference Standard / Sigma-Aldrich)

RELATED PROTEINS :

Testing method: HPLC (To assay the percentage of rhGH and the impure proteins in the product)

DIMMER AND RELATED SUBSTANCES OF HIGHER MOLECULAR MASS TEST :

Testing method: HPLC (To assay the percentage of active protein and the impure proteins in the product)


HAIZYIUAN HYGENE BIOPHARMA

Sample F (Jim's Lab AAA)

2.46 mg/vial

SIMEC - HPLC

2.87 mg/vial

----------------

GENLEI JINTROPIN

Sample G (Jim's Lab AAA)

2.80 mg/vial

SIMEC - HPLC

2.69 mg/vial

The SIMEC testing was done by Dr A (results seem close to AAA results) compared to the recent testing done by Matthias V (posted on PM)

I'm not in disagreement with Mands about the recent GH inaccuracies from SIMEC

Another HPLC where the standard is not mentioned, and since "Pharma" is considered GTG by many I can only hope theirs was NOT a Serostim, unless quantified by an AAA.

In fact this could be the reason SIMEC
results were noted to be "overdosed"
in the past.

 

[janoshik]

Another HPLC where the standard is not mentioned, and since "Pharma" is considered GTG by many I can only hope theirs was NOT a Serostim, unless quantified by an AAA.

In fact this could be the reason SIMEC
results were noted to be "overdosed"
in the past.

You brainless illiterate asshole "(HPLC (EP) Reference Standard / Sigma-Aldrich). "

I'm gonna bash SIMEC all day for their results, but one thing they cannot be accused of is not getting proper standards and they have no problem with providing invoices for those.

You are a joke.

 

[ProfessorX]

Another HPLC where the standard is not mentioned, and since "Pharma" is considered GTG by many I can only hope theirs was NOT a Serostim, unless quantified by an AAA.

In fact this could be the reason SIMEC
results were noted to be "overdosed"
in the past.

When Dr A was doing some HGH testing/HGH experimenting with 'JuicedMuscle'.....I think they may have used Hygetropin, as a comparison (standard)

For my testing, (anyone sending in GH sample for analysis) Dr A did order the correct pure standard :

Sigma Cat. no. S0947000 - somatropin reference standard (SIGMA-ALDRICH)

When I had 'BioP' do some quantitative testing they did it properly:

Determine quantity present in grams via HPLC-MS response of intact protein relative to calibration curve of Sigma somatropin standard Cat. no. S0947000

Determination of intact mass of provided sample.

Sigma Cat. no. S0947000 - somatropin reference standard

HPLC-MS of known quantity Sigma Cat. no. S0947000 - somatropin reference standard to generate 5-point calibration curve

I can see where you might think they were using a PHARMA (Serostim) Sample as a reference from the 'JuicedMuscle' articles

 

[janoshik]

I can see where you would think they were using a PHARMA Sample as a reference from the 'JuicedMuscle' articles

Interestingly enough that was not the post he had quoted...

 

[ProfessorX]

Interestingly enough that was not the post he had quoted...

Did I misinterpret his post? (i do that often )

There's some old postings (juicedmuscle) where they were doing some testing (comparisons)

I think they were comparing Hygetropin, JINTROPIN, Blue Tops and some other miscellaneous generics or counterfeits

It's a very old article

 

[janoshik]

I always assume people refer to the post they quote.

Also, according to my info, SIMEC really uses standards acquired from SIGMA, is ready to provide the info and generally I don't have a reason to distrust that. Unless of course JIM has insider info that SIMEC falsifies invoices now. JIM seems to be big on the insider info about everything.

 

[ProfessorX]

I always assume people refer to the post they quote.

Also, according to my info, SIMEC really uses standards acquired from SIGMA, is ready to provide the info and generally I don't have a reason to distrust that. Unless of course JIM has insider info that SIMEC falsifies invoices now. JIM seems to be big on the insider info about everything.

Oh...I must have totally misread all that LOL!

SIMEC uses SIGMA standards for individual testing or legitamate pharmaceutical testing

They are not using a known PHARMA Sample as a comparison

That would be cray cray for an accredited lab to do that

 

[Dr JIM]

Unless things have changed over the past FIVE years Sigma does NOT sell or supply rHGH as an analytical (HPLC, LC/MS etc) standard!

Fact is I contacted them last month to confirm the absence of a GH standard for analytical testing was not a web site sales error.

They do sell HGH for use in cell culture and other BIOLOGICAL assays.

Moreover by convention any analytical lab SHOULD reveal the standard being used in their worksheet or narrative discussion.

It should be accompanied with a lot number, manufacturers date, and serial number.

To that end I'm curious who told you SIMEC obtains their GH standard
from Sigma Aldrich?

 

[PrideofNYC123]

This is the standard simec is using according to Jano and ProfessorX. Correct me if I'm wrong.
Somatropin European Pharmacopoeia (EP) Reference Standard | Sigma-Aldrich


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[Dr JIM]

I'll have to look at it closer but noted it's a European standard that not for sale in the U.S.

The fact this type in info does not routinely accompany their research is troubling IMO.

 

[PrideofNYC123]

I'll have to look at it closer but noted it's a European standard that not for sale in the U.S.

Here is the US standard, though simec wouldn't use that since they are eu.

Somatropin United States Pharmacopeia (USP) Reference Standard | Sigma-Aldrich


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