sasquatchhunter
New Member
I let myself get to 20% this year!!!! I don't know if I believe the calipers though. I was looking at pictures of 20% and I just don't look like I have that much fat on me. Anyways my goal is 10-12% by May.
MESO-Rx
Anabolic Steroids
a few more GENERIC GH ASSAYS
- Thread starter Dr JIM
- Start date
I let myself get to 20% this year!!!! I don't know if I believe the calipers though. I was looking at pictures of 20% and I just don't look like I have that much fat on me. Anyways my goal is 10-12% by May.
muscle96ss
Subscriber
He has you two on block can't you tell. lol
On the SIMEC issue I think it was you @muscle96ss that stated they were not experienced with GH testing and they basically stopped GH testing for the moment when their results were posted on PM.
Correct me if I'm wrong? And 2014 is pretty damn new to testing in my book @ProfessorX
mands
I don't think they ever stopped testing GH. They did admit that it is very tough to test for GH in the manner that we were doing. They stated that their testing doesn't normally involve people sending them a vial and then trying to determine how much GH is in the vial. I am not sure what applications they use their testing for; but I have heard this from other labs before as well. In any event Jano as well as the lab that performed the immunoassay got the contents of the Serostim correct, while Simec was way off; so its obvious that there is something wrong with either their methodology or their tester. My guess is that it is their tester.
Also, while Jim may have me blocked; the details about Simec using EP(European Pharmacopeia) was posted by Prof X; so no excuses for him not comprehending it.
He has you two on block can't you tell. lol
On the SIMEC issue I think it was you @muscle96ss that stated they were not experienced with GH testing and they basically stopped GH testing for the moment when their results were posted on PM.
Correct me if I'm wrong? And 2014 is pretty damn new to testing in my book @ProfessorX
mands
I had communication with Mr. Vogt this morn.
They do test HGH for Purity and content Usin HPLC methods. I know HGH testin was a small portion of their business I did not ask if they ever stoppped testin My belief is they never have...
As far as How long they have been testin HGH this is the answer I was givin.."We do this since some years, I cannot give more specific answers to our internal things"
Kinda a shame that Dr. Jim has some on Block . My feel is that you are never to old or to educated, Or xperienced to learn from others.....
Kinda Ironic some censor others on a uncensored Board.
although controversial at times Dr. Jim is educational..
Big-Amsterdam
New Member
prof x, dr jim; how much would it cost me to have a vial of HGH tested?
its great to see these tests. amazing how much difference there is. from nothing to 150% overdosed... its realy a lottery with HGH.
its great to see these tests. amazing how much difference there is. from nothing to 150% overdosed... its realy a lottery with HGH.
Thank you for the clarification. My stance still remains the same on SIMEC and GH testing.
I agree. He has become a little different in a good way since his Uncle's passing recently. I have seen the nature of his posts change and this I like from him. He is a good man and has good intentions and has never lied to me. That's one thing I hate most is a liar(they can't be trusted) and we have one here at MESO and PM.
And I really wouldn't call it censoring as other members can see all post made.
As always, thank you @Buck1973
mands
sasquatchhunter
New Member
I don't know if Jim is a liar. I can't decipher half of his posts and I'm college educated. I do think he like many doctors have a hard time admitting they are wrong. Egos are a big deal. From my experience in life holding such ideas are a hinderence to my growth as a human both logically and emotionally...
That's a EUROPEAN STANDARD Jano and MH do either of you know the difference, apparently NOT! And to that end SIGMA does NOT sell a GH STANDARD for use in THE US! Call them and you will be referred to USP as I was!
Your attempts to discredit others will not enhance your own credibility and its obvious the latter is THE ONLY REASON you continue to post irrelevant nonsense and IS WHY I placed you and those like you "on block".
Adious
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janoshik
Subscriber
You were referring to European Company testing according to European standards.
Doctors have no more difficulty admitting they are wrong than some "patients" have admitting they are RIGHT.
And the reason many patients believe their Dr is "wrong" is often based on one factor,
their individual results or outcome. I wish it were that simple!
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Eman
Master
You're suddenly much less outspoken...
janoshik
Subscriber
Do you want me to write an assay on that? What else is there to write in response to that?
Eman
Master
You're quite thin skinned, aren't you?
janoshik
Subscriber
I never claimed otherwise
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JIM
Eman
Master
If you could just grow a pair of nuts you could actually be a contributing member...
janoshik
Subscriber
Which facts that I had posted were wrong?
janoshik
Subscriber
http://www.sigmaaldrich.com/catalog/product/usp/1615708?lang=en®ion=CZ&cm_sp=Insite-_-prodRecCold_xviews-_-prodRecCold10-6 (Somatropin United States Pharmacopeia (USP) Reference Standard | Sigma-Aldrich)
Not sure how it shows to you, but for me it shows as directly available to buy.
I think the issue might've been that you are not a registered business.
Over here Sigma only sells to those.
Attachments
It should be obvious NUTS are NOT an AAS forum prerequisite, lol!
Dang.....drama drama
Imma take your advice and try and filter out all that static and stick with the legit info thats available. Soooo.... anyway:
Jim,
I haven't gotten a response on those 4 samples (BioP) still out for testing. I'm assuming it won't be until after the holidays when I'll get the results
This is some info I got from another lab I recently reached out to
It seems that formulation is important and Glycine seems to be a potential issue with the data and results (depending on testing methods)
It doesn't seem that the Seros sample contained Glycine, but quite a few of the Chinese generics do (JINTROPIN and OMNITROPE did aswell)
Protein concentration in my sample
The appropriate analysis depends on the sample complexity and if the quantification needs to be absolute in picomoles & micrograms or just relative to other proteins & samples.
We offer a range of quantification services including:
- Absolute quantification of the purified protein batch.
- Relative quantification of multiple proteins in complex samples.
- Absolute quantification of single, individual proteins in complex samples.
We follow the principles for analysis of identity, purity, quantity, as well as product- and process-related impurities as described in the ICH Q6B guidelines: Test Procedures and Acceptance Criteria for Biotechnological/Biological Products.
Analysis Details
This Amino acid analysis refers to the method used to quantify proteins and peptides.
Sixteen amino acids are quantified – the normal 20 amino acids except tryptophan and cysteine. Asparagine is determined as aspartic acid and glutamine as glutamic acid. The analysis process includes:
- Protein hydrolysis
- HPLC analysis
- Data calculation
Apparatus
The analysis is performed on a BioChrom 30 amino acid analyser by ion-exchange chromatography with post-column derivatization using ninhydrin. All amino acids except Proline (440 nm) are monitored at 570 nm.
Protein hydrolysis – Vapour phase
The sample is placed in a 0.5 mL hydrolysis tube and dried down. The tube is placed in a vessel containing an acid hydrolysis solution (6M HCl containing, 0.1% phenol, 0.1% thioglycolic acid). The vessel is flushed with argon and after evacuation (<10 mBar) the vessel is placed at 110°C for 24 hours. After hydrolysis the test sample is dried in vacuum and the sample is dissolved in loading buffer (sodium citrate pH 2.20) including a fixed amount of sarcosine (Sar). This is used as an internal standard to correct for changes in reagent stability and flow rates, and the variation for each sample should not exceed 5%.
Calibration of Instrument
A standard mixture of amino acids is analyzed at 3 different concentrations levels (double determination) to show linearity within the measurement range (700 to 2800 pmol). A plot is made showing peak area versus known concentration of each standard solution. The r-value of the calibration has to be better than 0.999 (proline is accepted at 0.995 due to lower signal/noise). The instrument is linear to 5600 pmol and usually up to ~20 nmol. Depending on the amino acid residue, the signal goes into saturation around 25-35 nmol.
Accuracy & Precision
For each batch run we include a duplicate analysis of a NIST BSA quality control standard. The results are used to determine the precision and the accuracy of the test. The precision should be below 5% and the accuracy within 15%.
Data calculations
A standard amino acid solution is analyzed for each 5 samples, and based on at least two runs, a compensation factor is calculated to correct for ninhydrin activity. Each amino acid in the sample is corrected by this compensation factor.
Unknown protein samples: If a protein sequence is not given, the total protein amount is calculated based on the amount of each amino acid (in pmol) multiplied by the mass of the given amino acid residue. Please note that as the content of cysteine and tryptophan is not included, the obtained value is underestimated by approximately 2.7% (average Cys and Trp content in proteins in the Swiss-Prot database).
Known protein samples: If the protein sequence is known, the measured AA amount in pmol is divided by the number of each specific amino acid in the sequence providing a pmol protein. The average of these numbers in pmol is used to calculate the amount of protein in µg using the molecular weight of the protein.
The calculations against known amino acid sequence is done using a best linear fit based on all detected amino acids, but will be modified for each experiment not to include amino acids with greater than 5 per cent variation.
Lab states they have experience testing pharmaceutical formulated Somatropin
Depending on the results from BioP, I would like to try sending a few samples here
Just wanting your opinion.....this test is a bit more expensive and extensive than the $200-250 AAA
What I'm looking for is a lab I (we) can trust to produce accurate results
As you can see with the comparison results I've posted....all labs are not created equal (as you've correctly pointed out)
Even some of the SIMEC GH results have inconsistent results
The SIMEC results I've post here seem to be a bit more accurate than PMs results
Thanks
(I can always PM the lab info also)
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