a few more GENERIC GH ASSAYS

ProfessorX

Member
I'll have to look at it closer but noted it's a European standard that not for sale in the U.S.

The fact this type in info does not routinely accompany their research is troubling IMO.

I had to wait awhile for my first GH results from SIMEC because Dr A informed me he had to order the reference standard. I didn't get the exact info of what he ordered

In Jan, 2016.....BioP (U.S.) ordered:

Sigma Cat. no. S0947000 - somatropin reference standard

(Not sure why it would be the EP standard)(it's what was on my invoice)

They still have the reference standard. I'm sure I could get the exact info from BioP

Somatropin European Pharmacopoeia (EP) Reference Standard | Sigma-Aldrich

Other Info:

European Pharmacopoeia Reference Standards - EDQM

https://crs.edqm.eu/db/4DCGI/db/4DCGI/leaflet?leaflet=S0947000_3

Fully Traceable Absolute Protein Quantification of Somatropin That Allows Independent Comparison of Somatropin Standards | Clinical Chemistry

MILLARD also posted some info about SIMEC and their Reference Standards. SIGMA and others were listed
 
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muscle96ss

Member
I'll have to look at it closer but noted it's a European standard that not for sale in the U.S.

The fact this type in info does not routinely accompany their research is troubling IMO.

LOL!! Jim, Simec just happens to be located in Europe and claims to perform their work to EP(Eurpoean Pharmacopeia) standards. It is written as such in what Prof X posted and was fully disclosed by Simec. Do you actually read the posts before you spew your bullshit? There seem to be a lot of excuses here that are based on misinformation where the correct info is right in front of your eyes.
 

janoshik

Member
Unless things have changed over the past FIVE years Sigma does NOT sell or supply rHGH as an analytical (HPLC, LC/MS etc) standard!

Fact is I contacted them last month to confirm the absence of a GH standard for analytical testing was not a web site sales error.

They do sell HGH for use in cell culture and other BIOLOGICAL assays.

Moreover by convention any analytical lab SHOULD reveal the standard being used in their worksheet or narrative discussion.

It should be accompanied with a lot number, manufacturers date, and serial number.

To that end I'm curious who told you SIMEC obtains their GH standard
from Sigma Aldrich?

Do you feel the need to ashame yourself any more?
 

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janoshik

Member
I am going to love how JIM is not going to address all the valid arguments thrown at him at all.

Good night guys.
 

ProfessorX

Member
Simec is not new to that, I agree.

We've been looking and found a trail of samples that had shown a trend of being 'overdosed' by their results spanning long time back.

Mr. V. certainly did not leave a good impression on me when we dealt with him, but even going through his notes I was not able to determine where the problem could've been. Might be something as stupid as using a wrong pipette tip when working with standard in the end... And there's no real way to track that.

Jano,

What info can you give in relation to legit pharmaceutical GH sample results

For the samples that you have tested.....what has been the results (mg/VIAL) in relation to the labeled mg/VIAL

Serostim states that a % mechanical loss is associated with reconstitution and multi-dose administration

Just trying to figure out what is to be expected in mg/vial and what is acceptable

SIMEC tested an authentic:

ANSOMONE 3.33mg/VIAL
JINTROPIN 3.33mg/VIAL

BOTH TESTED 2.69 mg/VIAL

Is this result acceptable?

Does it fall within the acceptable Standard Deviation (SD)

We have lots of color top and "generic" brand results......just not many PHARMA Sample results to compare

Does the Liquid HGH test different than the Lyophilized HGH
 
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janoshik

Member
From pharma samples I've been testing multiple Humatrope vials, one Sero.

The highest deviation from stated content was 8% - and that was with sample that went through multiple dilutions, so adhesion could've artificially lowered the content. Since I've been using the different process to avoid issues like those it had been within 2%.

I've never tested Ansomone or Jintropin, so I can't say.

My standard deviation from the results, intra and inter assay had been within 0.01% since I've upgraded my setup, but I'm not 100% sure I'm calculating SD correctly, as I'm getting lost in statistics. Will PM you with the numbers after I get my breakfast.
 

ProfessorX

Member
From pharma samples I've been testing multiple Humatrope vials, one Sero.

The highest deviation from stated content was 8% - and that was with sample that went through multiple dilutions, so adhesion could've artificially lowered the content. Since I've been using the different process to avoid issues like those it had been within 2%.

I've never tested Ansomone or Jintropin, so I can't say.

My standard deviation from the results, intra and inter assay had been within 0.01% since I've upgraded my setup, but I'm not 100% sure I'm calculating SD correctly, as I'm getting lost in statistics. Will PM you with the numbers after I get my breakfast.

You sir are Awesome! :)

Even though I'm able to find an accredited lab (other than SIMEC) to accept my sample(s)....

It doesn't automatically mean I'm going to get the most accurate results...the lab might not have a lot of experience testing samples like what I'm sending

Not to mention my lack of communicating the correct terminology of the type of testing that I need

It plays into what Jim was saying about choosing the correct lab

I'm glad I asked this question...this is very helpful

I think what could help is including a 'control sample' with each batch of GH samples

A scientific control is an experiment or observation designed to minimize the effects of variables other than the independent variable.[1] This increases the reliability of the results, often through a comparison between control measurements and the other measurements. Scientific controls are a part of the scientific method


https://www.edqm.eu/medias/fichiers/bsp004_somatropin_crs1.pdf
 

janoshik

Member
I'll be forwarding you the data for pharma sample tested by SEC (SEC HPLC, SE HPLC) by me.

For examply I don't use intraassay, or internal control sample, but the machine is calibrated before each use and a control sample is ran in order to see, if retention time and area are still within the ranges acceptable for normal variation. Since getting an autosampler the variation is about 1/20th or less of the acceptable maximum, which is astonishing even to me.
 

mands

Member
AnabolicLab.com Supporter
LOL!! Jim, Simec just happens to be located in Europe and claims to perform their work to EP(Eurpoean Pharmacopeia) standards. It is written as such in what Prof X posted and was fully disclosed by Simec. Do you actually read the posts before you spew your bullshit? There seem to be a lot of excuses here that are based on misinformation where the correct info is right in front of your eyes.

I am going to love how JIM is not going to address all the valid arguments thrown at him at all.

Good night guys.
He has you two on block can't you tell. lol

On the SIMEC issue I think it was you @muscle96ss that stated they were not experienced with GH testing and they basically stopped GH testing for the moment when their results were posted on PM.

Correct me if I'm wrong? And 2014 is pretty damn new to testing in my book @ProfessorX :)

mands
 

janoshik

Member
He has you two on block can't you tell. lol

I can, but I think that my replies are appreciated by a part of the community at least and by calling JIM out on BS it can be noticed by more people that he lacks valid counterarguments.

Or any sense at all at times.
 
I can, but I think that my replies are appreciated by a part of the community at least and by calling JIM out on BS it can be noticed by more people that he lacks valid counterarguments.

Or any sense at all at times.

Jano,

Based on the assays thus far and what you believe. Would you use the Greys? Would you use the blacks?
 
To be honest I don't plan on using HGH at all, at least for now.

Greys would be my choice, though.

This is my first run with GH only on my second week and can't tell anything. 6iu of black, numb hands when I wake which I'm not sure if it is from that or me sleeping on my arm? Greys start in Feb. I do know it's gonna take months and I will go for 6 and see if I think it benefits me. There are so many other things happening i.e. I am in a caloric deficit and other compounds even at say the 5 month mark I doubt I'll know what is doing what.
I took my before pics just this morning. Biggest thing I want is an assist in my fat loss.
I just know my diet is what dictates that. Which has me wondering if I blew a ton of cash.... we will see...
 

janoshik

Member
You could consider lowering the dosage or getting off the product for a while, to see, if the problem persists.
 

Mr.B66

Member
AnabolicLab.com Supporter
This is my first run with GH only on my second week and can't tell anything. 6iu of black, numb hands when I wake which I'm not sure if it is from that or me sleeping on my arm? Greys start in Feb. I do know it's gonna take months and I will go for 6 and see if I think it benefits me. There are so many other things happening i.e. I am in a caloric deficit and other compounds even at say the 5 month mark I doubt I'll know what is doing what.
I took my before pics just this morning. Biggest thing I want is an assist in my fat loss.
I just know my diet is what dictates that. Which has me wondering if I blew a ton of cash.... we will see...

If fat loss is your main goal with GH then you have not wasted your cash IMO. GH along with a fairly clean diet will give you fat loos results unlike anything you have ever experienced , at least thats been my experience and i dont see why it wouldnt be the same for you. If i remember correctly , about the 2-3 month mark i started noticing fat loss.
Good luck and enjoy!!!!
 
You could consider lowering the dosage or getting off the product for a while, to see, if the problem persists.


The morning numb hands are not a bother. I just grab the arm and shake the hand and feeling comes back. If CPS kicks in and my lifts are effected I will lower my dose. As of right now not much in terms of anything to report. Just notice if I try to inject subq certain parts of the stomach have more nerve endings than others.
 
If fat loss is your main goal with GH then you have not wasted your cash IMO. GH along with a fairly clean diet will give you fat loos results unlike anything you have ever experienced , at least thats been my experience and i dont see why it wouldnt be the same for you. If i remember correctly , about the 2-3 month mark i started noticing fat loss.
Good luck and enjoy!!!!

I hope to see this! I really do. This weekend my diet won't be so good. But as a whole the past 7 days(when my bulk ended) has been much cleaner than the four months prior.
I went dirty bulk this year to 248 from 228.
 

Mr.B66

Member
AnabolicLab.com Supporter
I hope to see this! I really do. This weekend my diet won't be so good. But as a whole the past 7 days(when my bulk ended) has been much cleaner than the four months prior.
I went dirty bulk this year to 248 from 228.

Its all up to you, as long as your diligent in your efforts pertaining to diet and training you will see rhe results your after. BTW i was only pinning 2iu per night and went from about 16% to 8% in about 8 months. I took it slow so as not to loose muscle in the process. I was very satisfied with my results. Even posted pics in the biggest most shredded [emoji41]
 

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