Aegean Analyzer - Steroid Lab Tests

[rpbb]

His only problem is that, as I mentioned in other topic a few weeks before, the university staff doesnt have experience and they got some setbacks because of that. With help of you and guys like you that know what to do, he will set it all up and running soon hopefully.

If it wont be considered shill, I can get my lab tests published time to time, as I am planning to get plenty, even tho I am a bit worried getting unnecessary hammering because of that

go ahead and publish them

 

[master.on]

His only problem is that, as I mentioned in other topic a few weeks before, the university staff doesnt have experience and they got some setbacks because of that. With help of you and guys like you that know what to do, he will set it all up and running soon hopefully.

If it wont be considered shill, I can get my lab tests published time to time, as I am planning to get plenty, even tho I am a bit worried getting unnecessary hammering because of that

Thanks, @Turkish Pharmacy

I believe the university must have qualified chromatographers staff, if they did the previously posted tests.
At worst, they don't have any experience testing steroids or GH. But as long as they know how to use the machines, you can find a suitable testing method online for free. Then it becomes easy.

And yes, I'm glad to help if I can.

go ahead and publish them

Please publish them @Turkish Pharmacy

 

[TRT]

Bump. Or is there another thread started

 

[master.on]

@aegean analyzer do you have more reports to show us
right as they come from the HPLC machine?

 

[aegean analyzer]

@aegean analyzer do you have more reports to show us
right as they come from the HPLC machine?

Right now we are running tests on hgh and other substences for @Turkish Pharmacy and waiting shipment for other customers.

I am going to post reports asap when we have them.

 

[TRT]

Right now we are running tests on hgh and other substences for @Turkish Pharmacy and waiting shipment for other customers.

I am going to post reports asap when we have them.

What's the cost of testing?

 

[aegean analyzer]

What's the cost of testing?

Qualitative Analysis: 60 EUR
Qualitative + Quantitative Analysis: 90 EUR

You can check first post of this thread for other details

 

[master.on]

Qualitative Analysis: 60 EUR
Qualitative + Quantitative Analysis: 90 EUR

You can check first post of this thread for other details

Your GC-MS reports look fine.
Do you have some HPLC reports to show us?

 

[master.on]

I meant HPLC reports for steroids
not necessarily for GH.

 

[Inquiring Mind]

So it's not easily photoshopped.

Not that it makes any difference at all.

You would know.

 

[aegean analyzer]

Here is some hplc results from nandrolone decanoate sample during method development.

Your GC-MS reports look fine.
Do you have some HPLC reports to show us?

Here is some hplc-dad sample rt curves during last deca test. This curves belongs to standart substance (because we cannot trace any deca in last results if you look at them.

We tried hplc-dad to see how it reacts to our standart (during method development)

In few weeks we will receive new results form lab for tablet oxymetholone. I will post that results also.

 

[janoshik]

Here is some hplc results from nandrolone decanoate sample during method development.

Here is some hplc-dad sample rt curves during last deca test. This curves belongs to standart substance (because we cannot trace any deca in last results if you look at them.

We tried hplc-dad to see how it reacts to our standart (during method development)

In few weeks we will receive new results form lab for tablet oxymetholone. I will post that results also.

You have not attached anything

 

[aegean analyzer]

There was some problems with pdf format. Here are the images

 

[janoshik]

There was some problems with pdf format. Here are the images

Sigh.

These data were obtained by who? Professor T. herself?

 

[aegean analyzer]

Actually she is a senior researcher not professor
As ı told you folks those are some hplc readings only. Not results. @master.on asked for some of them and i published some demo. In few weeks I will publish hplc test results.

 

[Heavy Iron]

Test reports delivered between 2 to 4 weeks after sample recived.

So after shipping realistically we're looking at 1.5-2 months for results?

Edit: in total. Shipping+ testing

 

[janoshik]

Actually she is a senior researcher not professor
As ı told you folks those are some hplc readings only. Not results. @master.on asked for some of them and i published some demo. In few weeks I will publish hplc test results.

I asked, because it looks more like a work of a 1st year student than senior/professor. No offense to you, but offense to the person who did those tests. One should be ashamed of producing something like that, especially in senior position.

My opinion will be taken as biased around here, so I'll let others chime in, however the 'demo' shown shows less than undergrad level of knowledge in HPLC.

When @Turkish Pharmacy asked questions before, for the lab, I already had suspicion about their abilities, however, it is now confirmed.

There is pretty much no separation happening in the last image shown, it's more of a guessing game - pretty much anybody can see that it's impossible to determine the area under the curve precisely by a single look:


But more importantly the K Prime is around 0.3

Absolute minimum, often insufficient (with deca most certainly!) is 1.

FDA minimum is 2.

For deca I'd say 3+ is acceptable.

HPLC HINTS and TIPS FOR CHROMATOGRAPHERS: K Prime (also known as: Capacity Factor, Ratio or Retention Factor): One of the Single Most Important HPLC Parameters of All

Simply put, the results produced are invalid, unfortunately.

@XmadXscientist @ProfessorX

 

[master.on]

There was some problems with pdf format. Here are the images

Thanks for posting.

Some unbiased comments:

1 They look like legit HPLC reports.

2 No HPLC report can tell you if it's really Testosterone / Nandrolone (or any other substance) by itself (that's why GC-MS is used for identification).
So while they look like legit HPLC reports, I can't tell if they're for T/Deca indeed (nobody can).

3 While you might roughly identify steroids by their with a know method with Relative Retention Times, no method was disclosed
https://www.agilent.com/cs/library/applications/5991-8948_UHPLC-MSMS_AppNote.pdf
So I can't tell if they're T/Deca charts indeed by looking at their RTs.

4 Aegan used UPLC instead of HPLC, so fewer methods are available online for comparison.

5 UPLC is the way to go. Tests takes <5 minutes with UPLC as compared to the typical 15-20 minutes for HPLC.

6 @aegean analyzer can you please ask them how the peak height or Area Under Curve in the chart translated to concentration or purity % for raws?
i.e. what's the math behind it?
This is general info every customer is entitled to know.

I asked, because it looks more like a work of a 1st year student than senior/professor. No offense to you, but offense to the person who did those tests. One should be ashamed of producing something like that, especially in senior position.

My opinion will be taken as biased around here, so I'll let others chime in, however the 'demo' shown shows less than undergrad level of knowledge in HPLC.

When @Turkish Pharmacy asked questions before, for the lab, I already had suspicion about their abilities, however, it is now confirmed.

There is pretty much no separation happening in the last image shown, it's more of a guessing game - pretty much anybody can see that it's impossible to determine the area under the curve precisely by a single look:
View attachment 100734

But more importantly the K Prime is around 0.3

Absolute minimum, often insufficient (with deca most certainly!) is 1.

FDA minimum is 2.

For deca I'd say 3+ is acceptable.

HPLC HINTS and TIPS FOR CHROMATOGRAPHERS: K Prime (also known as: Capacity Factor, Ratio or Retention Factor): One of the Single Most Important HPLC Parameters of All

Simply put, the results produced are invalid, unfortunately.

@XmadXscientist @ProfessorX

The first two charts look fine. No way they're invalid in any way.
But I agree that peaks should better get sharper.

But I agree that the third one even has negative mAU.
I wonder if they switched the mobile phase (solvent) just to try it, I wonder why.
Why do we get negative peaks in HPLC?

I also wonder if they were trying to develop a method using another, less suitable column, the one that's already in the machine.
This is UG testing, so they can't ask management for a check to buy another column.
While Aegan could buy the proper column himself, but maybe he ain't allowed to open the machine to replace it.

I also wonder if they switched detectors. i.e. refraction instead of UV.

So after shipping realistically we're looking at 1.5-2 months for results?

Edit: in total. Shipping+ testing

Wait time is unavoidable for a proper lab
not only Aegan must wait so they can sneak the test in,

labs must also wait to gather enough samples of the same substance to make the most out of every reference standard.
Some pharma labs test 10 samples between each calibration run,
and that's for pharma QC
so for UG testing, you can expect 20, or 30+ sample runs between calibrations.



Bottomline:
So far @aegean analyzer looks like the ONLY legit UG testing lab in Meso at the current time.
Only equaled by former Analyzer.

 

[Sdryx]

Thanks for posting.

Some unbiased comments:

1 They look like legit HPLC reports.

2 No HPLC report can tell you if it's really Testosterone / Nandrolone (or any other substance) by itself (that's why GC-MS is used for identification).
So while they look like legit HPLC reports, I can't tell if they're for T/Deca indeed (nobody can).

3 While you might roughly identify steroids by their with a know method with Relative Retention Times, no method was disclosed
https://www.agilent.com/cs/library/applications/5991-8948_UHPLC-MSMS_AppNote.pdf
So I can't tell if they're T/Deca charts indeed by looking at their RTs.

4 Aegan used UPLC instead of HPLC, so fewer methods are available online for comparison.

5 UPLC is the way to go. Tests takes <5 minutes with UPLC as compared to the typical 15-20 minutes for HPLC.

6 @aegean analyzer can you please ask them how the peak height or Area Under Curve in the chart translated to concentration or purity % for raws?
i.e. what's the math behind it?
This is general info every customer is entitled to know.





The first two charts look fine. No way they're invalid in any way.
But I agree that peaks should better get sharper.

But I agree that the third one even has negative mAU.
I wonder if they switched the mobile phase (solvent) just to try it, I wonder why.
Why do we get negative peaks in HPLC?

I also wonder if they were trying to develop a method using another, less suitable column, the one that's already in the machine.
This is UG testing, so they can't ask management for a check to buy another column.
While Aegan could buy the proper column himself, but maybe he ain't allowed to open the machine to replace it.

I also wonder if they switched detectors. i.e. refraction instead of UV.




Wait time is unavoidable for a proper lab
not only Aegan must wait so they can sneak the test in,

labs must also wait to gather enough samples of the same substance to make the most out of every reference standard.
Some pharma labs test 10 samples between each calibration run,
and that's for pharma QC
so for UG testing, you can expect 20, or 30+ sample runs between calibrations.



Bottomline:
So far @aegean analyzer looks like the ONLY legit UG testing lab in Meso at the current time.
Only equaled by former Analyzer.

You mean Jano is LYING when he says the results are invalid??

I didn’t see that coming!

 

[janoshik]

You mean Jano is LYING when he says the results are invalid??

I didn’t see that coming!

I see you've chosen master.on as your source of professional knowledge.

It's not like I didn't know you are retarded, but you are truly working hard to shine.