Dirts Brew log

[narta]

1) So Kstek sell media bottle + topcap filter + vacuum setup all sterilized.

2) Then what I need after? A suction pump? Any link so I can have an idea on what to buy.

I 'll jump in here,

1) Yes
2) brake bleed pump

 

[Dirthand]

can you please tell me all the equipment you have bought to do the mediacap filter setup.

media bottle filter setup:
-Millipore .22 pvdf stericup bottletop filters
Use for 20%BB or less. Medlabsupply.com

-KS-TEK .22 nylon bottletop filters/w sterile media bottle. KS-TEK aliexpress
- brake bleeder kit (vacuum pump) Amazon
-glass media bottles medlabsupply
- Open top gl45 caps medlabsupply
- ptfe silicone septa medlabsupply

The septa I have I do not care for Sometimes they do not seem to close back up well. @nartPosted a link yesterday about sime in aliexpress that looked gangster.. i am going to try them.

Was the Deca 400 pipless? Still holding at 20% BB

Yes , but I have only done 1 shot at 70mg for my cruise. However I did commit a sin and used 2% guaiacol.. but do not tell anyone..

What about the test D 500 with 25% BB, pipless as well?

Only did 1 shot at 200mg yes pipless for me..

Same with the masteron 350 and Tren 250?

Emptied a couple vials a peice at the end of my cycle zero pip next time I will possibly try tren e at 300 but do not think I would ever go higher than that

castor oil

I tried castor oil to make some tne to come up with a recipe without EO an guaiacol.. although some people do well with castor I do not.. it hurts me so I threw the bottle I bought in the trash..

 

[Dirthand]

I 'll jump in here,

1) Yes
2) brake bleed pump

Sorry I just woke up and it took me like an hour to type all that. This guy right here fellows is a great guy.. I wear his ass out with dumbass questions and he never fails to help me. Thanks Mr. Narta..

 

[Sampei]

View attachment 294889media bottle filter setup:
-Millipore .22 pvdf stericup bottletop filters
Use for 20%BB or less. Medlabsupply.com

-KS-TEK .22 nylon bottletop filters/w sterile media bottle. KS-TEK aliexpress
- brake bleeder kit (vacuum pump) Amazon
-glass media bottles medlabsupply
- Open top gl45 caps medlabsupply
- ptfe silicone septa medlabsupply

The septa I have I do not care for Sometimes they do not seem to close back up well. @nartPosted a link yesterday about sime in aliexpress that looked gangster.. i am going to try them.

Yes , but I have only done 1 shot at 70mg for my cruise. However I did commit a sin and used 2% guaiacol.. but do not tell anyone..

Only did 1 shot at 200mg yes pipless for me..

Emptied a couple vials a peice at the end of my cycle zero pip next time I will possibly try tren e at 300 but do not think I would ever go higher than that

I tried castor oil to make some tne to come up with a recipe without EO an guaiacol.. although some people do well with castor I do not.. it hurts me so I threw the bottle I bought in the trash..View attachment 294892

To me Tren E 250 more then enough, 1.5ml a week is plenty...

Ohhhh so you cheated with GU on the Deca400 hopefully it will work with 20BB without EO or GU


Thank you for the media bottle filter setup, what's the minimum amount you can filter with that ? 100ml? 200?

 

[Dirthand]

To me Tren E 250 more then enough, 1.5ml a week is plenty...

Ohhhh so you cheated with GU on the Deca400 hopefully it will work with 20BB without EO or GU


Thank you for the media bottle filter setup, what's the minimum amount you can filter with that ? 100ml? 200?

the ones i have from kstek are 250ml rated but hey have other options 500ml, 1000ml ect. the Millipore is rated at 1000. So listen though i do the media bottle thing but when i get ready to put in smaller vials i use syringe filter also. The reason i do this is well beside(slight retardation) is when i first started i was just using the syringe filter and kept getting floaters. so i switched to bottle top and sometimes still had a couple of floaters.. by doing it both ways i have failed to find 1 single floater. The nylon filters take a while to filter .. probly because they are protein binding matbe... not sure but the work well for me and if you can EVER find STERILE PTFE bottletop filter anywhere shoot let me know.. i have exhaustedly looked. I do not use the sterile bottles that come with the filters from ks tek because they are plastic and im not sure if thats cool or not! maybe someone can chime in on that.. i vacuum seal them sealed for later..

 

[Dirthand]

Ohhhh so you cheated with GU on the Deca400 hopefully it will work with 20BB without EO or GU

im sure it will my raws were clumpy and were not disolving well so i put the 2% in there and cheated.. it worked well..I definantly do not promote the use of super solvents but will not lie about it if or when i do use them.

 

[Sampei]

How many beakers I need and of what size? I was thinking 100ml 250ml 500ml 600ml?

Bought graduated cylinder as well.

Hot plate separated from the magnetic stirrer or all together?

 

[Dirthand]

How many beakers I need and of what size? I was thinking 100ml 250ml 500ml 600ml?

i got a set from 10ml to 1000ml..

Bought graduated cylinder as well.

i have some but dont use them i use disposable,20,30, 60ml and 100ml syringes to measure.. im lazy and dont want to have to clean extra stuff

Hot plate separated from the magnetic stirrer or all together?

its one and the same

 

[Sampei]

i got a set from 10ml to 1000ml..

i have some but dont use them i use disposable,20,30, 60ml and 100ml syringes to measure.. im lazy and dont want to have to clean extra stuff

its one and the same

What beakers do you use more usually? Do you use sterile syringe instead of the graduated cylinder or you use non sterile?

 

[Dirthand]

What beakers do you use more usually? Do you use sterile syringe instead of the graduated cylinder or you use non sterile?

500 250 100.. sterile

 

[ThinkTrenbolone]

Thanks for actual showing the equipment. Rare to find good pics these days

 

[Sampei]

500 250 100.. sterile

Could one use non sterile syringes instead of the cylinders? I mean you are sterilizing the gear anyway after.

 

[Dirthand]

Could one use non sterile syringes instead of the cylinders? I mean you are sterilizing the gear anyway after.

I guess you can.. I have boxes and boxes of sterile ones they are pretty cheap . I wouldn't even know if there was a price difference..

 

[Sampei]

I guess you can.. I have boxes and boxes of sterile ones they are pretty cheap . I wouldn't even know if there was a price difference..

do you use surgical drapes to cover the table you are working on?

 

[ksm2]

and if you can EVER find STERILE PTFE bottletop filter anywhere shoot let me know..

qpore® bottle-top filter, PVDF, 250 ml, 0.22 µm, Ø 50 mm 24 pcs./pack

qpore® offers a comprehensive range of bottle-top filters made of different materials, which are particularly suitable for the filtration of larger sample volumes. All membranes are manufactured under the highest quality standards from the best raw materi

www.neolab.de

They come without media bottles though

 

[Dirthand]

do you use surgical drapes to cover the table you are working on?

No painters paper only because I can wad everything up easy that way

 

[Dirthand]

qpore® bottle-top filter, PVDF, 250 ml, 0.22 µm, Ø 50 mm 24 pcs./pack

qpore® offers a comprehensive range of bottle-top filters made of different materials, which are particularly suitable for the filtration of larger sample volumes. All membranes are manufactured under the highest quality standards from the best raw materi

www.neolab.de

They come without media bottles though

Those are pvdf..

 

[ksm2]

Those are pvdf..

Right, i'm retarded. I will look again after getting some sleep

 

[ksm2]

Looks like your only option for sterile PTFE botte tops is some korean labstore. All the other vendors (Autofil, Labforce and Celltreat) offer it only as non sterile.

If i have to guess, i would say the ulabs that use PTFE bottle tops just get the non sterile ones and yolo it. Not that i would recommend it, but i can't imagine them actually caring that much.

Anyways, order at your own risk lol

PTFE-H (Hydrophilic Polytetrafluoroethylene)

http://www.hdmicro.co.kr/en/?c=193&s=&gp=1&gbn=viewok&ix=2108

and

PTFE-D (Hydrophobic Polytetrafluoroethylene)

http://www.hdmicro.co.kr/en/?c=193&s=&gp=1&gbn=viewok&ix=2105

 

[Exhortae]

Sterility testing for 240 USD - TAMC and TYMC test checking the presence of bacteria and molds in the product.

GCMS or LCMS analysis for 170 USD - blind test for contaminants, which is able to identify presence of harmful substances in the product. Those tests do not give concentration of such substances, however, it can be offered for additional price once such substance is detected. They do not check for heavy metals, bacteria, molds or LPS, which require specific test below


these are 2 test jano offer.I have never seen any ugl or anyone post results foe this except I seen someone post test for endotoxins on peptides. After talking with my sister whom I found out has the capability to perform these test she said she would for me. The sterility test will take about 3 weeks she said.. has anyone had these test performed before?

I do sterilty testing for bacteria and fungus at a biological labs near me.

Costs me 12 usd and i get the results after 4 days

But I live half of the year in a third world country. Not sure in europe they would agree.

I put the oil in a 1 ml sterile vial. I write on the sticker vitamin b12 and an expiration date.