Dirts Brew log

[kain]

I’ve been putting glass media bottles (and any other glass item) in the oven at 550F for 10 min to dry heat sterilize. First time I forgot to take off the plastic ring... I rinse with isopropyl alcohol, then distilled water before putting the item in the oven.

 

[Dirthand]

I’ve been putting glass media bottles (and any other glass item) in the oven at 550F for 10 min to dry heat sterilize. First time I forgot to take off the plastic ring... I rinse with isopropyl alcohol, then distilled water before putting the item in the oven.

Thank you brother

 

[Dirthand]

Using a pressure cooker as an autoclave - PubMed

Using a pressure cooker as an autoclave

pubmed.ncbi.nlm.nih.gov

Thank you brother!

 

[kiss-my-axe]

Amazon has a bunch of options for autocalves

 

[Spaceman Spiff]

When I got into brewing years and years ago I went this route as well.... After that first 100ml I never did it again lol.

This method is super easy lol imo. Can custom brew concentrations easily

 

[Dirthand]

Amazon has a bunch of options for autocalves

You reckon! I was looking into alternative methods with the resources I have on hand. Appreciate the heads up tho bro!

 

[Dirthand]

This method is super easy lol imo. Can custom brew concentrations easily

I kept having trouble with the end that plugs onto the filter kept breaking and I had to go through 3 or 4 hoses. I was probably trying to push it on too tight. And then i blew out a filter.. just wasnt aware of the amount of pressure i was putting it under but I'm learning.

 

[Spaceman Spiff]

I kept having trouble with the end that plugs onto the filter kept breaking and I had to go through 3 or 4 hoses. I was probably trying to push it on too tight. And then i blew out a filter.. just wasnt aware of the amount of pressure i was putting it under but I'm learning.

once you get it, it will be easy. can use multiple caulking guns so it does a slow drip method.

 

[Akc83]

I’ve been putting glass media bottles (and any other glass item) in the oven at 550F for 10 min to dry heat sterilize. First time I forgot to take off the plastic ring... I rinse with isopropyl alcohol, then distilled water before putting the item in the oven.

I was under the impression that dry heat sterilization had to be low heat around 250 for a couple hours with aluminum foil on top of vials for evaporation. I know there’s more than one way to skin a cat. Am I wasting time by doing low heat and baking longer? I buy non sterile vials and give them the 99% iso alcohol bath rinse distilled water and line them all up on a baking sheet open end up put aluminum foil sheet on top and bake low and slow. I also have an autoclave but have yet to use it, for some reason it scares the shit outta me building pressure. It came with no instructions from china so hopefully you see my concern.

 

[Dirthand]

once you get it, it will be easy. can use multiple caulking guns so it does a slow drip method.

I purchased some bottle top filters and a pump but I feel like I would need to do brews of at least 200-300ml for that method.. I have no idea what I would do with that much dope. But I figure I could filter it into media bottles and I bought some open caps(those Dam things are expensive) and some silicone plugs so I can just draw it out as needed.

 

[HereToTeachAndLearn]

I was under the impression that dry heat sterilization had to be low heat around 250 for a couple hours with aluminum foil on top of vials for evaporation. I know there’s more than one way to skin a cat. Am I wasting time by doing low heat and baking longer? I buy non sterile vials and give them the 99% iso alcohol bath rinse distilled water and line them all up on a baking sheet open end up put aluminum foil sheet on top and bake low and slow. I also have an autoclave but have yet to use it, for some reason it scares the shit outta me building pressure. It came with no instructions from china so hopefully you see my concern.

Here is an abstract of a study which determined 150C for one hour should suffice:

Dry-heat sterilization of parenteral oil vehicles - PubMed

The purpose of this study was to evalutate the effect of temperature and time on the dry-heat sterilization conditions of cottonseed, peanut and sesame-seed oils used as vehicles for parenteral drugs. The three oils were inidividually spiked wiht Bacillus subtilis spores and exposed to dry heat...

pubmed.ncbi.nlm.nih.gov

 

[Deskoze38192]

Here is an abstract of a study which determined 150C for one hour should suffice:

Dry-heat sterilization of parenteral oil vehicles - PubMed

The purpose of this study was to evalutate the effect of temperature and time on the dry-heat sterilization conditions of cottonseed, peanut and sesame-seed oils used as vehicles for parenteral drugs. The three oils were inidividually spiked wiht Bacillus subtilis spores and exposed to dry heat...

pubmed.ncbi.nlm.nih.gov

You already posted this in this thread

The person you quoted was asking about sterilization of glass vials. He was not asking about dry heat sterilization of carrier oils which we do not do in home brewing.

 

[HereToTeachAndLearn]

You already posted this in this thread

The person you quoted was asking about sterilization of glass vials. He was not asking about dry heat sterilization of carrier oils which we do not do in home brewing.

You win the prize for superior intellect and I win the prize for failing to read on a 5th grade level. My apologies, Sir!

 

[HereToTeachAndLearn]

You already posted this in this thread

The person you quoted was asking about sterilization of glass vials. He was not asking about dry heat sterilization of carrier oils which we do not do in home brewing.

Some people do dry heat sterilize carrier oils, but obviously not you! Congratulations!

What amazes me is how some people are so stuck in their ways, that they fail to believe anything different can be of benefit. Instead of accepting that there could be scientific, potential harm reduction reasons for doing things differently, they just blindly try to argue that there is no reason to do so. This level of ignorance is unbelievable!

Each person will have their own level of tolerance for harm reduction and there is absolutely nothing wrong with that.

You do you!

 

[iris]

You win the prize for superior intellect and I win the prize for failing to read on a 5th grade level. My apologies, Sir!

Some people do dry heat sterilize carrier oils, but obviously not you! Congratulations!

You are just being passive aggressive and he does not deserve your useless sarcasm, just because he pointed out the obvious.

The person you quoted was asking about sterilization of glass vials

You can't recognise what you meant in this instance was misguided.
There's one thing for you to learn.


So I see you edited your message, adding a lot, including:

Each person will have their own level of tolerance for harm reduction and there is absolutely nothing wrong with that.

You do you!

Exactly.
And when you wrote about it, nobody took the mickey out of you

 

[Akc83]

You win the prize for superior intellect and I win the prize for failing to read on a 5th grade level. My apologies, Sir!

So you’re not smarter than a 5th grader… sorry my intellect isn’t as superior but you still haven’t answered the question

 

[HereToTeachAndLearn]

The answer to his question can be found here, or rather one version of an answer.

https://www.medisca.net/pdf/shortage-support/en-us/injection/F%20004%20571v2%20Testosterone%20100%20mg%20per%20mL%20Intramuscular%20Injection.pdf

 

[Deskoze38192]

Some people do dry heat sterilize carrier oils, but obviously not you! Congratulations!

What amazes me is how some people are so stuck in their ways, that they fail to believe anything different can be of benefit. Instead of accepting that there could be scientific, potential harm reduction reasons for doing things differently, they just blindly try to argue that there is no reason to do so. This level of ignorance is unbelievable!

Each person will have their own level of tolerance for harm reduction and there is absolutely nothing wrong with that.

You do you!

When you first joined you argued with a chemical biologist about the use of heat as he was trying to explain the benefit of using as little as possible when brewing
In this thread your “advice” caused the OP to overheat his brew and eventually decide to toss it.
You are still very much in the learning stage but are pushing your beliefs on to others because you read about something about a study.
I mean no harm or offense and you are entitled to your beliefs and methods but do not push them on others when there are specific known reasons not to use those methods in the brewing process.
There have been very knowledgeable and experienced brewers that have explained that your “dry heat sanitization method” is not needed or recommended.

 

[HereToTeachAndLearn]

When you first joined you argued with a chemical biologist about the use of heat as he was trying to explain the benefit of using as little as possible when brewing
In this thread your “advice” caused the OP to overheat his brew and eventually decide to toss it.
You are still very much in the learning stage but are pushing your beliefs on to others because you read about something about a study.
I mean no harm or offense and you are entitled to your beliefs and methods but do not push them on others when there are specific known reasons not to use those methods in the brewing process.
There have been very knowledgeable and experienced brewers that have explained that your “dry heat sanitization method” is not needed or recommended.

Ignorance is bliss!!! Do research, and then ask yourself why Bayer sold one of their testosterone patents for 500 million pounds a couple of years ago. That 500 million pound patent references dry heat sterilization.

Look at the above document. It also references terminal dry heat sterilization.

How can you be so ignorant of information that is readily available?

You don’t have to agree, but accept that there is also evidence that is contrary to your established beliefs.

Unbelievable!

 

[DECLAN]

Can I afford 500million? I guess I am getting additional ass holes soon lmao.