Enhanced Testing Database

[Ghoul]

You are too kind. And here I was thinking I'm just a whiny c*nt, lol.

Now @Ghoul, that's a different matter. A knowledge KING.

You do the heavy lifting around here.

I just set heads on fire.

 

[janoshik]

Some more beauty.

0.999 Calibration Curve and 0.1 and 0.01 aas oil spike.

 

[-Maui-]

Holy shit first time we see something that is not 0.00x

@janoshik could this be because you are doing it in house now? Compared to the old tests

Hi! Do you really have samples with a 0.00x level?
Can you show them? It's interesting.
Thanks!

 

[readalot]

Hi! Do you really have samples with a 0.00x level?
Can you show them? It's interesting.
Thanks!

Check out the thread. Jano recently brought the method in house. My suspicion is the original testing outside his lab was confounded by oil matrix. He has since solved that issue with proper endotoxin recovery method from the oil as part of the assay.

I'll post some links if you are truly curious.

 

[readalot]

@-Maui-

R

Post in thread 'Janoshik Analytical laboratory testing services'

Jan 29, 2025

endotoxin test on AAS oil

See below. While you are interacting via email could you inquire about item 2?

Fyi...

Open questions

1. Calibration method on water based endotoxin analyses. @Ghoul the method procedure was provided above along with the info on assay kit used.

2. Recovery method used to extend endotoxin analysis to oil based products [OPEN]. Jano invited someone to contact his company to get more info.

...

• readalot

Example from Project 2

R

Post in thread 'Readalots Enhanced Testing'

Nov 18, 2024

PROJECT 2 coming soon.

Raws HPLC testing, metals, and endotoxins.

Focus was on the raws but we got some finished oil vials and lyophilized GH vial in there as well.

I want to thank @Sampei very much for his collaboration on this project. His contributions to further our body of knowledge as a community is simply incredible. He donated the 9 raws, finished oil vials, and a lyophilized GH vial. He did all the heavy lifting and got the samples to Jano. We went in half on the HPLC testing and I covered the remainder of the tests.

Quote number #xxxxx ...

• readalot

• 
• 

I caught alot of shit early on that all these samples "were free from endotoxins". Still don't look too bad.

 

[readalot]

Some more beauty.

0.999 Calibration Curve and 0.1 and 0.01 aas oil spike.View attachment 317026

Sweet!

 

[Ghoul]

@-Maui-

R

Post in thread 'Janoshik Analytical laboratory testing services'

Jan 29, 2025

endotoxin test on AAS oil

See below. While you are interacting via email could you inquire about item 2?

Fyi...

Open questions

1. Calibration method on water based endotoxin analyses. @Ghoul the method procedure was provided above along with the info on assay kit used.

2. Recovery method used to extend endotoxin analysis to oil based products [OPEN]. Jano invited someone to contact his company to get more info.

...

• readalot

Example from Project 2

R

Post in thread 'Readalots Enhanced Testing'

Nov 18, 2024

PROJECT 2 coming soon.

Raws HPLC testing, metals, and endotoxins.

Focus was on the raws but we got some finished oil vials and lyophilized GH vial in there as well.

I want to thank @Sampei very much for his collaboration on this project. His contributions to further our body of knowledge as a community is simply incredible. He donated the 9 raws, finished oil vials, and a lyophilized GH vial. He did all the heavy lifting and got the samples to Jano. We went in half on the HPLC testing and I covered the remainder of the tests.

Quote number #xxxxx ...

• readalot

• 
• 

I caught alot of shit early on that all these samples "were free from endotoxins". Still don't look too bad.

It would also be wise to consider that the limits placed on endotoxin are based on not inducing an immediate fever.

It's becoming increasingly clear that cumulative endotoxin exposure over a lifetime, without symptoms, leads to all sorts of health problems from arthritis to dementia.

You'd be hard pressed to find a group that injects a greater volume of unregulated, underground produced liquids than we do.

More than any heroin addict.

That's why it keeping it to a minimum is in everyone's interest, if you plan on living a long healthy life.

 

[readalot]

It would also be wise to consider that the limits placed on endotoxin are based on not inducing an immediate fever.

It's becoming increasingly clear that cumulative endotoxin exposure over a lifetime, without symptoms, leads to all sorts of health problems from arthritis to dementia.

You'd be hard pressed to find a group that injects a greater volume of unregulated, underground produced liquids than we do.

More than any heroin addict.

That's why it keeping it to a minimum is in everyone's interest, if you plan on living a long healthy life.

Looking forward to see @janoshik 's generous offer of a quarterly report out after we get some more data with his validated in house method!

 

[Ghoul]

Looking forward to see @janoshik 's generous offer of a quarterly report out after we get some more data with his validated in house method!

The beauty of your campaign, is that as members from here influence other boards and online groups, "Where's the endotoxin test?" may very well become the expected standard in short order, just like dimer testing quickly became a must have for hgh.

And good for Jano's business too, since this is likely out of reach for most other labs to do properly.

 

[janoshik]

Looking forward to see @janoshik 's generous offer of a quarterly report out after we get some more data with his validated in house method!

We've so far had only one product with outright dangerous levels of endotoxin and that was recombinantly manufactured saccharide raw powder

 

[readalot]

The beauty of your campaign, is that as members from here influence other boards and online groups, "Where's the endotoxin test?" may very well become the expected standard in short order, just like dimer testing quickly became a must have for hgh.

And good for Jano's business too, since this is likely out of reach for most other labs to do properly.

And kudos to you big time for continuing to daylight the oil matrix concern. I should have done a better job there before too many tests were done with v1.0.

 

[AlexDavis43]

Some more beauty.

0.999 Calibration Curve and 0.1 and 0.01 aas oil spike.View attachment 317026

That is a good-looking standard curve. Also giving me PTSD from thousands of hours pipetting.

 

[janoshik]

That is a good-looking standard curve. Also giving me PTSD from thousands of hours pipetting.

I had to have fancy pipettes express delivered because my lovely CSO and her assistant were threatening me with a strike after 10 hours of pippetting straight with not-so-fancy pipettes.

 

[Spaceman Spiff]

I had to have fancy pipettes express delivered because my lovely CSO and her assistant were threatening me with a strike after 10 hours of pippetting straight with not-so-fancy pipettes.

you're a pipette

 

[janoshik]

you're a pipette

Apparently not fancy enough

 

[readalot]

Some more beauty.

0.999 Calibration Curve and 0.1 and 0.01 aas oil spike.View attachment 317026

So now that you have a calibrated in house endotoxin method with proper recovery, what do you recommend on the prior generation of testing that all showed endotoxin levels below LOD?

I believe you mentioned you have some type of guarantee with the third party lab with respect to their testing. I'm concerned the first generation of testing gave the community a false sense of confidence with respect to the endotoxin levels in oil based gear.

Would you offer a repeat testing complimentary or at reduced price for the prior testing contained in this thread? This is partly my fault for not properly ensuring you had a validated method.

I'd appreciate your consideration of this situation.

 

[FastBun]

So now that you have a calibrated in house endotoxin method with proper recovery, what do you recommend on the prior generation of testing that all showed endotoxin levels below LOD?

I believe you mentioned you have some type of guarantee with the third party lab with respect to their testing. I'm concerned the first generation of testing gave the community a false sense of confidence with respect to the endotoxin levels in oil based gear.

Would you offer a repeat testing complimentary or at reduced price for the prior testing contained in this thread? This is partly my fault for not properly ensuring you had a validated method.

I'd appreciate your consideration of this situation.

This is a good request… I think comped repeat testing would be fair… would generate more testing once these ugl start getting exposed…

 

[Spaceman Spiff]

Let's say hypothetically this was really done a year ago with the manufacturing testing. After all these shutdowns it shows that consistent enhanced testing would have been a pointless crusade.


We can't even get a hold of raws that easily lol.

All the "enhanced testing bought" was probably done on a manufacturer that didn't exist anymore.


For some reason I thought this thread was supposed to be dead/given up

 

[readalot]

For some reason I thought this thread was supposed to be dead/given up

You thought wrong. You mean you wanted it to be dead/given up? Does it appear dead/given up?

Selling an improperly validated/calibrated assay and relying on that data is an issue. You understand this.

Hence, one compromise is to allow a credit on future endotoxin testing by the same persons/vendors who purchased the first test. Therefore getting a new set of benchmark data.

 

[Spaceman Spiff]

You thought wrong. You mean you wanted it to be dead/given up? Does it appear dead/given up?

Selling an improperly validated/calibrated assay and relying on that data is an issue. You understand this.

Hence, one compromise is to allow a credit on future endotoxin testing by the same persons/vendors who purchased the first test. Therefore getting a new set of benchmark data.

It's pretty clear this place is their world, not mine. If there was any significant support for this we would have seen it by now. The last 10 pages here tell the story. I've taken this testing bit about as far I will be able to. Perhaps someone with better approach can salvage something out of it.

There is such little support,

even neo anabolics is just posting general HPLC testing to advertise itself. lol