You measured using a beaker? Use a syringe or graduated cylinder. Beaker isn't nearly as preciseHonestly the only thing I can think is the beaker from med lab supply was inaccurate. That’s the only thing that makes sense. I’m going to buy another beaker from a different science supply company and see if they are identical in the amount of fluid they hold at the 100ml mark. Because the hormone was fully dissolved and I kept it stirring as I was filtering. I know I didn’t stir it long, But it’s the only conclusion I can come up with because I’m good at math.