I'll look around and do more research, I have info from ppl that capped their own vial without any flow hood or hepa filters and they never had issues but I prefer to be on the safe side.
MESO-Rx
Anabolic Steroids
How do you filter castor oil?
- Thread starter Sampei
- Start date
Spaceman Spiff
Well-known Member
I feel like it would be a bigger pain in the ass to use a bottle top filter like that for PERSONAL use. but it would be fun to learn
If you use a bottle top filter, how do you then get the oil into the sterilized vials without air contamination?
Spaceman Spiff
Well-known Member
food for thought
When they do open surgeries, there is a sterile field and no flow hood above the open body.
well you can't use syringe filters and cap your own vials UNLESS you don't mind not having a flip off cap... I do mind tho. I like pretty flip off cap.
So the next step is syringe filters into media bottle with septum and then draw, fill vials, cap vials straight away.
All of this is for personal use, I love the idea of having all my 10ml vials with flip off on ahaha
Yeah I'm retarded I know.
Spaceman Spiff
Well-known Member
You absolutely can.
get a decapper lol. leave the stopper on there. Make sure it stays closed to keep the inside sterile
Sure, but operating rooms tend to have very expensive air treatment and ventilation systems installed. Doors usually closed while operating, etc. I'm sure this doesn't remove all bacteria but probably more sterile than my house.
The YouTube videos I've watched of pharma facilities making oils are also using a clean room with HEPA ventilation.
I suppose a flow hood could work. I used one of those back when I was working on my chemistry undergrad degree a 100 years ago (before dirt was dirt). My question is whether a laminar flow hood is enough. People use them for growing mushrooms, but is it okay for brewing injectibles? I guess I'll keep reading.
Edit: I found something called "terminal heat sterilization" so I will research that next.
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so you mean just buy pre sterilized, decap them, and recap them.
Yeah that's one way to do it.
Or I can sterilize my own vials, put the stopper on, inject the oil through the stopper and then cap it, the injection for filling can be done directly from a syringe filter (that takes time tho) or I can filter everything into a media bottle using syringe filters and then aspire with a syringe and fill the vial with the content of the syringe through the stopper as you said and then cap it.
I don't really wanna start using bottle top filter, it's a waste of money for me. Max I'll brew in one go is 100ml. Bottle top filter come in no less than 150ml and it's 20$ each, I can filter a lot more gear with 20$ of syringe filters.
For big quantity it gets a lot cheaper the bottle top, 500ml are 30$ each.
Spaceman Spiff
Well-known Member
I mean, I guess you can put the stopper on. I probably could save money by dry heat sterilization and an autoclave. Maybe I should consider that lol
you don't need both, if you buy silicone PTFE/stopper you can dry heat vials and stoppers together
or you can just isobath them if they are butyl stopper, many ppl says it's enough
tcri
New Member
I was going to say, "very slowly."
And it sounds like I'm being a smart ass, because I am. But, I mean no harm. It just seems like you experienced the viscosity.
And I have nothing valuable to offer, I'm sorry.
Musmadar
Member
In surgery room they use ultraviolet+ozone lamps for air sterilization ...I wonder if this could be used for ours settings.???
Probably but imagine the cost lol
I bough an air purifier yesterday, wife looked and me and said: I believe this project is getting a little bit out of hand...
I couldn't answer back because... Fuck... Yeah it went from: it's just 300-400$ of equip and it's game on... To I'm not sure but I believe I need this nuclear reactor for some gamma radiation sterilization
Musmadar
Member
A uv+ozone lamp is not that expensive...I have one that cost me around 50€...it's good enough to sanitize a regular room, in 10 minutes the air is totally different and the smell of ozone is strong enough to say the job is done.
Oh I thought a medical grade hospital level kinda of things. Yeah I'm thinking to buy one, ozone is not good to be breath tho for what I remember so you gotta air the room afterwards wouldn't that counter the sterilization thing? I was thinking to pair it with the air purifier, I'm not sure tho if I should keep the air purifier on while the UV lamps sterilize or have the air purifier start when the UV lamps has finished. Trying to find an answer to that
Musmadar
Member
Maybe it's a good matter to start debating on...
Yeah indeed, consumer UV lamps can't sterilize far away tho so for example it's better to keep it very close to the place/tabletop you gonna brew, run it for the time it needs and then keep an air purifier on so that the room continue to be as sterile as possible.
Enter the room after the sterilization have everything set up before so that you just start brewing, best is to clean with bleach and then iso before doing everything then final sterilization with UV lamp + air purifier the whole time (or after the UV lamp has finished).
This one way to do it, anorher one could be to sterilize with bleach than iso, start brewing WHEN you are ready to cap the vials and fill, you keep the brew warm and sterilize everything with UV lamp + air purifier and then you cap it. I mean one way or another it's really a lot more then average UGL is probably doing xD
Next step after this is getting a laminar flow hood, you get that one you are set for life. Can't beat that shit, is it necessary? Probably not, but sure as hell I'm gonna buy one when I get my home built, it's too cool ahahha
I read a lot yesterday about air purifier
Best for a medium small room is: Levoit Vital 200s or Winix 5500-2. Price are 150-200$ and it works well.
Keep it at maximum intensity 30-45 minutes before brewing and during the whole time.
Cunt check PM
jj89
Well-known Member
One thing I found to help with using syringe filters for those who don't know, don't fill the syringe all the way to the max, leave some space for an air gap between the oil and the plunger.
When you press down on the plunger you compress the air trapped above the oil and it does a lot of the work as long as you keep some pressure on the plunger.
When you press down on the plunger you compress the air trapped above the oil and it does a lot of the work as long as you keep some pressure on the plunger.
Bought a consumer UV lamp, AI perplexity says you can pair it with an air purifier but only if the flow of air is not disrupted by the air purifier.
than later on it says to make the UV lamp more effective there should be a flow of air ahahah so it's a bit confusing.
I'll do it this way: UV lamp alone than as soon as it finishes, start the air purifier and keep it going the whole time. end of story.
Air purifier will be kept near the brewing station and the UV lamp will be placed in a way that it does shine on the brewing station specifically during the sterilization process of 45/60 minutes.
Best I can do. total cost 200 euro. Not cheap, not horrible either.
I'll not be able to see any effect from it but If I don't get an infection while capping my own vials I can say maybe it helped xD
than later on it says to make the UV lamp more effective there should be a flow of air ahahah so it's a bit confusing.
I'll do it this way: UV lamp alone than as soon as it finishes, start the air purifier and keep it going the whole time. end of story.
Air purifier will be kept near the brewing station and the UV lamp will be placed in a way that it does shine on the brewing station specifically during the sterilization process of 45/60 minutes.
Best I can do. total cost 200 euro. Not cheap, not horrible either.
I'll not be able to see any effect from it but If I don't get an infection while capping my own vials I can say maybe it helped xD
