McLovin.
New Member
next up a vacuum cleaner pulling vacuum in the vial pulling the oils through the syringe filter
Hi Narta can you explain this setup a bit more? i looked up silicone seplas and am not sure of their purpose,
fucking hell, i am so embarassed. I spent days and days looking to find this exact thing but the only stoppers i could find were from a pharma company, ill link pic below.
You can sterilize the gl45 glassware and silicone stoper, converting the gl45 receiver as a giant sterile vial. Use a long ass needle to transfer the filtered gear to smaller presterilized vials.
I don't use bottletops but anything reputable that has a gl45 neck would do. Opt for PTFE hydrophilic with PVDF being a second option. .22micronsfucking hell, i am so embarassed. I spent days and days looking to find this exact thing but the only stoppers i could find were from a pharma company, ill link pic below.
Thanks so much Narta you are a gentleman and a scholar.
Would you mind linking the bottle top filters you use? Also what kind of needle do you use to draw from the bottom of big ass GL45 bottles
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I can get it all pre sterilized here in Germany right from the manufacturer. But to my understanding if I take the bottle top filter off the glass bottle and put the pre sterilized silicone stopper on it there is a small risk of contamination by not using a flow hood, right? Like some unfiltered air is going in during the 2-3 seconds of changing the bottle top filter to a silicone stopper.
You can sterilize the gl45 glassware and silicone stoper, converting the gl45 receiver as a giant sterile vial. Use a long ass needle to transfer the filtered gear to smaller presterilized vials.
I am happy enough to take the chance of a tiny bit of unsterile air getting into the bottle tbh.I can get it all pre sterilized here in Germany right from the manufacturer. But to my understanding if I take the bottle top filter off the glass bottle and put the pre sterilized silicone stopper on it there is a small risk of contamination by not using a flow hood, right? Like some unfiltered air is going in during the 2-3 seconds of changing the bottle top filter to a silicone stopper.
Thats the actual reason I am still using vials and syringe filters as I have no place to hide a flow hood this size anywhere, my son will find it and I honestly can not tell if its a risk or not to do it without a flow hood.
What kind of filter do you use Narta if you dont mind me asking? I have seen some glass ones that use disposable filters, would you reccomend one of them? Seems like they would be much more economical in the long term compared to single use bottle tops.I don't use bottletops but anything reputable that has a gl45 neck would do. Opt for PTFE hydrophilic with PVDF being a second option. .22microns
As for needles you don't need long ones, just think that the gl45 with the septa is a big ass vial, so just flip and draw
There are long needles but usually they need sterilization between each batch and it's a pita. Search for 100mm dispensing needles
There is virtually no danger if you don't have air circulating, sneeze, fart or spit on dat thang.Like some unfiltered air is going in during the 2-3 seconds of changing the bottle top filter to a silicone stopper.
I use syringe filters my volume per batch rarely exceeds 200ml for mutiple rawsWhat kind of filter do you use Narta if you dont mind me asking?
No the sand core ones are a pita to clean and sterilize properlyI have seen some glass ones that use disposable filters, would you reccomend one of them?
There are apparatuses that can adapt for gl45 bottles search on AliExpress for JoanlabI have seen glass ones that need to be clamped on to the bottle, do you think one of them would work with a threaded GL45 bottle?
I found the one below, it seems suitable to what i am thinking. it uses disposable filters.I use syringe filters my volume per batch rarely exceeds 200ml for mutiple raws
No the sand core ones are a pita to clean and sterilize properly
There are apparatuses that can adapt for gl45 bottles search on AliExpress for Joanlab
Could you please link the vacuum buchner filter attachment that you use?Hand rinse with soap>dishwasher>ISO wash>pressure cooker
I dont even do all that myself
I like using gl45 media bottles with a vacuum buchner filter attachment as soon it's done I just hand swap to a sterilized gl45 septa cap. I also use sterile borosilicate glass syringes to measure out my 10ml volumes into vials if I break it down further. If it's just for personal use you can literally just use the oil straight out of the GL45 media bottle lol
Thanks for the advice, much appreciated, i think i will go for disposable bottle tops to start with for convenience.There's literally a million dude just Google "gl45 vacuum filter" and pick something that you can easily source .22 micron filter membranes for. If you wanna dabble in this hobby you're gonna have to expend the time to research properly and pick a setup you're comfortable with. Make sure it's autoclavable, and not a fritted one. Fritted filters are designed for powderized filter cakes using celite not filter papers.
Just get pressure cooker they're like 40 bucks and dedicate it to your glassware only don't cook food in it