PEP test-e contaminant testing?

The only means of determining a cause and effect relationship using any culture technique is to recover the same organism in a patients wound or abscess.

But I must mention there is NO EVIDENCE that supports the notion bacterial gear contamination causes PIP.

First while PIP is relatively common site infections are NOT.

Second because the responsible organisms are almost always Staph and Strep, without sub-typing, proving the UGL is responsible party, rather than the patients poor technique, approximates the impossible.

I know bro lore dictates the cause of PIP is the "ester" being used but again NOTHING supports this contention either.

So what does cause PIP you ask? Look no further than the AAS powder guys.

Now Jim how can that be after all my source double or triple filters their powders.

Ok right, assuming that does in fact occur (and the better UGLs do exactly that) the etiology is the number of times the final product is distilled, precipitated and reconstituted.

Ya see each time this triple phase process is performed a variable quantity is "lost" in the primary solution, and that lowers the WEIGHT available for SALE.

I'll give one example easily detected using MS or HPLC

Thanks Dr. Jimmy. Am I correct in assuming that it would be extremely hard to have MRSA survive away from skin and in a vial of oil and some concentration of benzoyl alcohol, and that a MRSA infection would be far more commonly attributed to staph on the skin being plunged into muscle during injection; as is the case with most insect bites that turn MRSA?
 
During the production of Primo BROMIDE is used followed by LITHIUM. These agents are used to "open" the double bond of the third benzene ring allowing the insertion of the required alkyl group to form Primo.

However each step requires "washing" to remove the dissociated Bromide. Because otherwise the dissolved "free Bromide" will combine with YOUR H-2-O to form BROMIC-Acidor OR in the case of Lithium the formation of Lithium -Hydroxide.

The former is a relatively strong acid while the latter is a relatively strong base (ever heard of Lithium batteries)

Ok so NOW YOU KNOW WHY PRIMBOLAN is so damn expensive..... the "washing" required for a purified product and the "waste" which occurs as a result!
 
To finish with the bro PIP dogma.

We know the PRIMSRY causes of "PIP" when drugs are used for medicinal purposes.

Either the osmotic forces causing tissue distention or the PH of the product itself being less than neutral.

(Somewhere around a Ph of 7.0 or grater than 8.0)
(The generation of free radicles may also be causative, especially when "alcohols" are the preservative or the solvents used)

Regarding your question KS?

Unfortunately the BA concentration used for AAS (1-2%) should be considered bacteriostatic rather than bacteriocidal. (As I mentioned above want some "PIP" bump that BA concentration to 3-4%)

While BA at a
concentration of 1-2% MAY be bacteriocidal in an aqueous media that's certainly not the case in oils where uniform dispersion is quite limited AND utilization by the relatively dormant bacteria approximates ZIPPO.

What does BA do then?

It prevents bacterial reproduction as does the oil, since VERY few microbes fixate triglycerides as a metabolic energy. source.

Jimmy
 
Powerful Dr. Jim schooling the masses. Thank you sir! Very insightful stuff.
I should change my major to bio chem and start brewing. Lol I kid I kid...
 
Would you recommend tryptic soy over blood
When I get ready in the next few weeks is it cool if I pm you

Blood agar is rich medium, not necessarily TSA, you can use it I do not know how much it costs but I imagine with the blood it is more expensive...Sure go ahead and shoot me a PM.
 
To finish with the bro PIP dogma.

We know the PRIMSRY causes of "PIP" when drugs are used for medicinal purposes.

Either the osmotic forces causing tissue distention or the PH of the product itself being less than neutral.

(Somewhere around a Ph of 7.0 or grater than 8.0)
(The generation of free radicles may also be causative, especially when "alcohols" are the preservative or the solvents used)

Regarding your question KS?

Unfortunately the BA concentration used for AAS (1-2%) should be considered bacteriostatic rather than bacteriocidal. (As I mentioned above want some "PIP" bump that BA concentration to 3-4%)

While BA at a
concentration of 1-2% MAY be bacteriocidal in an aqueous media that's certainly not the case in oils where uniform dispersion is quite limited AND utilization by the relatively dormant bacteria approximates ZIPPO.

What does BA do then?

It prevents bacterial reproduction as does the oil, since VERY few microbes fixate triglycerides as a metabolic energy. source.

Jimmy
Your the man Jim...Testing the gear will give piece of mind to the masses if it comes back clean. As I said before I would be surprised if there were microbes, the environment is a difficult one.
 
Welcome back Doc, I may even look over the fact you still refer to yourself in third person ,some emergency medicine doctors' god complex, with the information and knowledge you share with us !!!!
Thank you
 
For those interested you will locate Blood agar plates are available via Google for about a buck per plate.
(Blood agar is a nice general purpose media especially for gram positive microbes such as Staph and Strep)

However just remember when "streaking" you AND everything else which comes into contact with the plate must be sterile.

(Providing the plate is open for only a few seconds the concern for ambient air droplet contamination is MUTE!

Why? Because contamination of that kind will
be far removed from the streaked area..... which is diagnostic of aerosol dispersion = tainted colony!

FYI

I've been involved in teaching for close to twenty years and have discovered using the "third person" is ONE OF MANY ways to communicate so others may learn.

(It also limits the need for transitional sentence adjectives or adverbs, which I certainly appreciate :) )

JIM
 
Just get a large bottle of trypticase soy agar it will last forever, it's cheap, and it stores for a ling time in the fridge. You aren't trying to find out if you have strep throat, the most common use of blood agar, you are trying to grow bacteria.
 
Just get a large bottle of trypticase soy agar it will last forever, it's cheap, and it stores for a ling time in the fridge. You aren't trying to find out if you have strep throat, the most common use of blood agar, you are trying to grow bacteria.

Personally I like the ease of prepped and sterile sheep's blood soy agar plates, and the added nutrient value makes it a bit easier at home when one doesn't have access to a precision controlled warm temp environment because from my understanding, because of the nutrient value of the sheep's blood, cultures will grow easier at room temperature. This could be mistaken, but I seem to remember being told that in some bio sciences class of another...

Anyways, my laziness and lack of sterile environment means I don't want to try and pour my own sterile plates, but I completely agree that your way is cheaper! :D
 
Typically, the only difference in blood agar is 5% sheep's blood. This is used to differentiate between bacteria that lyse(breakopen) red blood cells. It's very difficult to grow pathogenic bacteria at room tempurature, they thrive inside bodies of animals where the temp is 37° C. KISS( keep it simple stupid) lol.
 
Unfortunately the BA concentration used for AAS (1-2%) should be considered bacteriostatic rather than bacteriocidal. (As I mentioned above want some "PIP" bump that BA concentration to 3-4%)

My (slightly off-topic) question is why is BA so bro-popular vs. chlorobutanol?
 
Typically, the only difference in blood agar is 5% sheep's blood. This is used to differentiate between bacteria that lyse(breakopen) red blood cells. It's very difficult to grow pathogenic bacteria at room tempurature, they thrive inside bodies of animals where the temp is 37° C. KISS( keep it simple stupid) lol.

I'm lucky enough that on top of my fridge I'm probably in the ~32* C range. You think that's acceptable, or do I need to invest in a craigslist wine warmer?
 
uploadfromtaptalk1390845789164.jpg
These small dots are 24 hour contamination colonies for a reference.
uploadfromtaptalk1390845862912.jpg
uploadfromtaptalk1390845878849.jpg
I am going to make a slide right now to determine if this is bacteria. To me it looks clean, the cloudyness is most likely moisturized oil. It does not smell like a bacteria, I will take a closer look. Note the positive control, what you typically see in contamination is a set colony and I am not seeing that.
 
Back
Top