Qingdao Sigma Chemical Co., Ltd (International, US, EU, Canada and Australia domestic

[Ghoul]

What are the losses encountered when filtering though? Doesn't the filter hold back a lot of the solution? Even .25 ml held up absorbed into the filter would be significant no? Have you measured the before/after volume after filtering say a 2ml reconstitution solution?

That's an excellent question. From what I've found a typical 25mm syringe filter has a .05ml hold up volume.

13mm has a .01ml hold up volume.

Also important to ensure the filter uses a "low protein adhesion" membrane.

Finally, doing an "air purge" into the vial will push most retained liquid out of the filter.

Since smaller aggregates act a "seeds", attracting more peptide to build themselves, you're also likely preserving more unaggregated peptide than losing in the tiny amount of solution retention.

 

[Ghoul]

Here's one of the more detailed reports on protein aggregation. I can't possibly absorb all of it and go down every tangent. For instance they discuss the role of Benzyl Alcohol in multi-vial doses causing aggregation.

One interesting aspect of this has been a focus on why there's such a wide difference in efficacy and side effects between Pharma brands of HGH. Differences in packaging and handling, therefore amounts of aggregation may be the explanation as to why the same peptide has such different results. For instance, single use HGH doses use sterile water for reconstitution. Multi dose products use BAC, containing the (potentially) aggregate encouraging Benzyl Alcohol. Just food for thought...

Aggregation of protein therapeutics enhances their immunogenicity: causes and mitigation strategies

Protein aggregation in biotherapeutics has been identified to increase immunogenicity, leading to immune-mediated adverse effects, such as severe allergic responses including anaphylaxis. The induction of anti-drug antibodies (ADAs) moreover enhances drug clearance rates, and can directly block...

pubs.rsc.org

 

[sushak]

That's an excellent question. From what I've found a typical 25mm syringe filter has a .05ml hold up volume.

13mm has a .01ml hold up volume.

Also important to ensure the filter uses a "low protein adhesion" membrane.

Finally, doing an "air purge" into the vial will push most retained liquid out of the filter.

Since smaller aggregates act a "seeds", attracting more peptide to build themselves, you're also likely preserving more unaggregated peptide than losing in the tiny amount of solution retention.

So you are reconstituting, filtering entire solution into a new vial and then it's g2g? What stops further aggregation from happening post filtering? Especially if it's BA related?

Seems to me the only way to be sure would be to filter each and every shot, which would be prohibitively expensive for most.

 

[Underworld]

Diabetes with kidney failure.

not sure if srs

 

[Ghoul]

So you are reconstituting, filtering entire solution into a new vial and then it's g2g? What stops further aggregation from happening post filtering? Especially if it's BA related?

Seems to me the only way to be sure would be to filter each and every shot, which would be prohibitively expensive for most.

I've had to make peace with the old adage; "Don't allow perfect to become enemy of the good".

Would filtration immediately prior to use be ideal? No. Filtering right after reconstitution into a sterile, pyrogen free vial to eliminate as many aggregate "seeds" as possible to slow the development of new ones, THEN syringe filter again immediately prior to use would be "Ideal", This concept, called "Bedside filtration" is already in practice in some hospitals, and has demonstrated unquestionable better patient outcomes.

This is even further out of reach for what most would be willing or able to do.

Then again, if we're talking someone using a GLP weekly this could be done for under $3/month, given that appropriate filters can be had for under 50¢.

Picking one, the filter immediately prior to use "Bedside" model is likely the better option, but more expensive and time consuming.

Filtering once after reconstitution into a new vial is still a major improvement over not filtering at all, and if that's as far as someone wants to go they'll still reap benefits.

Do what you can, or want to do, or nothing at all. The point is to define "Best Practice" , using the latest knowledge, and let individuals decide what they want to do. Some folks only eat organic chicken. I don't. Would it be better for me? Probably, certainly unlikely to be worse.

To each his own.

 

[Docsunshine23]

I've had to make peace with the old adage; "Don't allow perfect to become enemy of the good".

Would filtration immediately prior to use be ideal? No. Filtering right after reconstitution into a sterile, pyrogen free vial to eliminate as many aggregate "seeds" as possible to slow the development of new ones, THEN syringe filter again immediately prior to use would be "Ideal", This concept, called "Bedside filtration" is already in practice in some hospitals, and has demonstrated unquestionable better patient outcomes.

This is even further out of reach for what most would be willing or able to do.

Then again, if we're talking someone using a GLP weekly this could be done for under $3/month, given that appropriate filters can be had for under 50¢.

Picking one, the filter immediately prior to use "Bedside" model is likely the better option, but more expensive and time consuming.

Filtering once after reconstitution into a new vial is still a major improvement over not filtering at all, and if that's as far as someone wants to go they'll still reap benefits.

Do what you can, or want to do, or nothing at all. The point is to define "Best Practice" , using the latest knowledge, and let individuals decide what they want to do. Some folks only eat organic chicken. I don't. Would it be better for me? Probably, certainly unlikely to be worse.

To each his own.

I will say man you are super fucking intelligent and I like that you do your own research.

The bedside filtering isn’t practical, I’ve been involved in many hospital systems across the country and the only units that have done it are the oncology floors. A few ICUs tried to but that never worked out given the immediate need.

Is this best practice? Fuck yeah. But are juice heads and peptide monkeys gonna do it? I’d say 0.4% might. I think if we could filter out the dimer in HGH without affecting the rest there would be a bigger desire at least here.

But I’ve enjoyed both of your most recent post.

Ok now I’ll take your dick out of my mouth, happy Saturday all.

 

[Ghoul]

I will say man you are super fucking intelligent and I like that you do your own research.

The bedside filtering isn’t practical, I’ve been involved in many hospital systems across the country and the only units that have done it are the oncology floors. A few ICUs tried to but that never worked out given the immediate need.

Is this best practice? Fuck yeah. But are juice heads and peptide monkeys gonna do it? I’d say 0.4% might. I think if we could filter out the dimer in HGH without affecting the rest there would be a bigger desire at least here.

But I’ve enjoyed both of your most recent post.

Ok now I’ll take your dick out of my mouth, happy Saturday all.

60% of ICUs and neonate departments in the US have this policy now, at least for IV drips, because it cuts the average patient stay down by 1 day. Less inflammation, fewer incidents of organ failure, fewer complications generally.

Of course that's for a number of reasons beyond protein aggregation. Sediment is thing with many meds, .22 filtration at the last minute also corrects for any earlier errors causing a loss of sterility in the parenteral medication, etc.

But hey, some folks reuse syringes, don't wipe vial tops, etc. I'm not judging. I'm sure there's a wide variety of practices folks have adopted to suit their risk tolerance.

It's useful to know what fact-based practices one can incorporate into their routine if they choose to, and reducing protein aggregates is just another tool in the box if someone wants what it offers.

 

[BuildABro]

60% of ICUs and neonate departments in the US have this policy now, at least for IV drips, because it cuts the average patient stay down by 1 day. Less inflammation, fewer incidents of organ failure, fewer complications generally.

Of course that's for a number of reasons beyond protein aggregation. Sediment is thing with many meds, .22 filtration at the last minute also corrects for any earlier errors causing a loss of sterility in the parenteral medication, etc.

But hey, some folks reuse syringes, don't wipe vial tops, etc. I'm not judging. I'm sure there's a wide variety of practices folks have adopted to suit their risk tolerance.

It's useful to know what fact-based practices one can incorporate into their routine if they choose to, and reducing protein aggregates is just another tool in the box if someone wants what it offers.

I suspect most people that don’t have access to a highly controlled environment along with the knowledge and strict adherence to safety protocols would try some shit like this and just end up doing more harm than good.

So what you are left with in the vast majority of cases is just precautionary theatre.

 

[narta]

I still think than ParalysisByAnalysis is more appropriate than Ghoul

 

[BuildABro]

I still think than ParalysisByAnalysis is more appropriate than Ghoul

I think maybe he should take up, oh I dunno, maybe lifting weights to get some hobby diversity? I have heard this activity is popular around these parts

 

[Ghoul]

I suspect most people that don’t have access to a highly controlled environment along with the knowledge and strict adherence to safety protocols would try some shit like this and just end up doing more harm than good.

So what you are left with in the vast majority of cases is just precautionary theatre.

A couple of decades ago many homebrewers denounced the concept of filter sterilization as "theater", because everyone knew boiling is what killed bacteria. duh.. That, along with the "common sense" knowledge that you don't use freshly made gear immediately to give the Benzyl Alcohol time to sterilize the vial contents.

This isn't rocket science for most. It's no more difficult than using a larger gauge to draw and switching to a smaller gauge to pin, a common practice many use daily.

 

[Ghoul]

I still think than ParalysisByAnalysis is more appropriate than Ghoul

I think maybe he should take up, oh I dunno, maybe lifting weights to get some hobby diversity? I have heard this activity is popular around these parts

What happens when the 2 special ed kids accidentally wander into the AP class.

Notice folks, it's not enough for them to ignore it, they want to make sure you can't read it either.

 

[narta]

What happens when the 2 special ed kids accidentally wander into the AP class.

Notice folks, it's not enough for them to ignore it, they want to make sure you can't read it either.

When your mom said you were special it didn't mean bright son. So don't self project your google-fu as knowledge.

Fun fact about being special: it doesn't mean you are useful. You are the annoying little rug that no one bothers to throw away because it can be used once in a while

 

[Ghoul]

When your mom said you were special it didn't mean bright son. So don't self project your google-fu as knowledge.

Fun fact about being special: it doesn't mean you are useful. You are the annoying little rug that no one bothers to throw away because it can be used once in a while

By "annoying" you mean I remind you of how fundamentally stupid you are.

Otherwise you'd just hit the ignore button and remove my posts from your sight, instead of pretending you're speaking on behalf of the majority, demanding I stop posting.

Just do it man. Hit ignore, pussy.

 

[BuildABro]

By "annoying" you mean I remind you of how fundamentally stupid you are.

Otherwise you'd just hit the ignore button and remove my posts from your sight, instead of pretending you're speaking on behalf of the majority, demanding I stop posting.

Just do it man. Hit ignore, pussy.

I do not see anyone suggesting anything in particular, only making observations. Observations which you have clearly taken offense to as evidenced by your last couple paragraphs of ad hom that would be right at home on the playground at recess

Not exactly a defining characteristic of utter brilliance that you are attempting to convince everyone you possess.

People talk lots of shit here, as I am sure you are well aware of. If this is not to your liking then I am sure there is some FB peptide group out there for you to impress with your long diatribes

 

[narta]

By "annoying" you mean I remind you of how fundamentally stupid you are.

Otherwise you'd just hit the ignore button and remove my posts from your sight, instead of pretending you're speaking on behalf of the majority, demanding I stop posting.

Just do it man. Hit ignore, pussy.

You could do the same, but your narcissistic ass can't cope with that. You like the attention.

Fun fact: It ain't raining, it's just everyone pissing at you dum-dum

 

[pipo]

This guy has a life besides copy paste non stop what google say on a forum ? Dude take a break. This is becoming a obsession to post and check a forum.
No ones gives 2 shits about you or what you post. Get a break. It's for your own good.

If we make a pool on how many actually read your post and want to continue to share your copy paste knowledge you will be surprise what results would be

 

[Sampei]

By "annoying" you mean I remind you of how fundamentally stupid you are.

Otherwise you'd just hit the ignore button and remove my posts from your sight, instead of pretending you're speaking on behalf of the majority, demanding I stop posting.

Just do it man. Hit ignore, pussy.

I'm still waiting for where to buy those special vials that don't delaminate. Just saying.

 

[zmurphy]

Yes it is. Use 3mL of BAC to reconstitute, and I strongly suggest you filter the contents into a sterile 3ml vial.

I'm curious, how much of the 3ml do you lose in the filter?

 

[Ghoul]

This guy has a life besides copy paste non stop what google say on a forum ? Dude take a break. This is becoming a obsession to post and check a forum.
No ones gives 2 shits about you or what you post. Get a break. It's for your own good.

If we make a pool on how many actually read your post and want to continue to share your copy paste knowledge you will be surprise what results would be

Only a moron would think familiarizing yourself with a topic like this is "google cut and paste". I reduce it to as succinct (quick, google this word) of an explanation as possible and provide what I think is the best of 100+ papers on the subject..

Despite reducing it to a relatively easy to understand concept, there will always be the product of damaged genetics, barely able to string together a sentence, stepping up like he's ever brought anything of value to MESO.

I get "X is now following you" notifications and an endless stream of DMs from intelligent, curious people every single day. You don't have one person interested in your ideas.

Since you're new here, let me help you out. Click my username and hit this button. You'll never see my posts again.



See, I even gave you a picture, do you think you can figure out how to complete this task without more help, retard?