@Sampei and
@max187 thanks for the positive reinforcement
Here you go:
>>Filtration with reusable equipment (standard lab equipment):
This is a pretty straightforward setup on Amazon and pretty inexpensive:
Amazon.com
You’ll want to find filter media that is appropriate, and the filter media may or may not be sterile. It usually isn’t. Personally, I doubt anybody is gonna die from something on a piece of filter paper BUT, if you’re concerned, cook the filter paper in the oven with your other lab equipment when you sterilize everything.
Here are some PTFE filter media for the above 80mm Buchner filter:
This:
Amazon.com
Or:
Amazon.com
Or:
Amazon.com
Filter media is designed to be a one time use; you dump the filter paper/media once you’re done filtering.
Typically, in a lab, you KEEP the filtrate that accumulates on the paper, and so what you’d do would be to set up your apparatus, make sure that it doesn’t tip over, as it’s top heavy, and then you’d put the filter media in and wet it down with a liquid that’s the same as the liquid you’re trying to filter out. So, let’s say you’ve got a beaker of water + salt and you want to filter out the salt. You’d put the filter media in, wet it with water, proceed to filter, and then take out the filter media with the salt on it and chuck the water.
In this use case, you want to TOSS the paper and any filtrate that accumulates on it, and KEEP the oil. So if you REALLY wanted to be quantitative this is what you’d do:
- Buy two of the above set up and set aside the extra buchner funnel as a spare.
- Get everything set up, put your buchner funnel over an Erlenmeyer flask.
- Put the filter paper/media in the funnel, and wet it down with a bit of PURE oil, ideally already filtered. This is just to get the filter media stuck down to the buchner funnel. It shouldn’t take much.
- Turn on the vacuum pump and suck the oil through to make sure you’ve got the filter media saturated, and any oil dripped through is collected in the flask below.
- REMOVE the flask that has a bit of pure oil in it, and replace it with the second cleaned/sterilized flask.
- Proceed to filter your solution.
You’d do this because any oil that comes through as part of the initial process will dilute the concentration of the solution (gear) you’re wanting to actually filter.
Personally, I wouldn’t likely bother with all that, and would simply carefully wet the filter media with the SOLUTION, knowing a few drops of unfiltered oil may get under the filter media and in to the flask below. But if every step to that point has been pretty careful, you’re pretty unlikely to have an issue. Again, depends on how quantitative you want to be with this. If you’re challenging yourself to make a concentration that is EXACTLY 100mg/ml for example, then you will want to make sure no extra oil gets in to your flask, not even a drop, because then it’ll change things. But really, a few drops will make your gear 98mg/mL instead of 100mg/mL, if that makes sense. So again, you may not care, especially if you test the final product.
Not sure if this is what you’re after, and hopefully it helps. I used to be a _______ teacher (redacted), for what it's worth
Added: additionally, if you don’t want to put filter media in the oven, you could rinse with isopropyl alcohol, or hydrogen peroxide, and let dry. That won’t sterilize, but it will disinfect/sanitize. You can really geek out on perfection at every step to your liking, but the early steps are the most important steps (make sure both solute and solvent are as pure as possible to begin with, and all glassware is disinfected/sanitized or sterilized, depending on your risk tolerance).