Save your unusable raws Guaranteed.

[Walter Hype]

I do membrane testing with FTM and TSB. Use PTS for the endo. Used to do LAL reagent in he uv before I got the PTS. I had to waste so much so I got the PTS.

Look into ozone hydrogen vapor sterilization. It's a new tech and it's fda approved for what im saying. The beauty of it is even if there's no off the shelf machine one can purchase for this ( still too new for the most of society to understand it). Some countries are adopting it quickly to stop using ethylene oxide. It's very efficient

You don't need a lot to make it work

A modified vaccume chamber. A oxygen concentrator. A ozone generator. (Quartz generator that accepts pressured air). And various tubing that can withstand the ozone. The hydrogen vapor can be be generated using a ultra sonic humidifier before hand. Run it in the chamber to saturate it. Concentration can be 15%. Treat it exactly like an autoclave cycle. Except instead of pressure your doing vac after you push some ozone in there in cycle of 5 psi in for 5 mins. Then pull a vac. The. Release. Push more ozone. Do it 3 times. This will displace all the air with only ozone even in filter paper


It sounds convoluted but it's just turning a few levers and switches.....but the venting part is trick I run a tube outside so it vents out and not into my lab.....ozone at this high of concentration makes you light headed and you will get deadly sick. But a vent with a tube is enough to fix that.



The beauty of this method is it needs zero heat. Yet it works as an entire sterilization cycle and yes dental paper back autoclave bags are compatible!


It's also faster than autoclave and no drying cycle.

 

[Thorshammer]

Look into ozone hydrogen vapor sterilization. It's a new tech and it's fda approved for what im saying. The beauty of it is even if there's no off the shelf machine one can purchase for this ( still too new for the most of society to understand it). Some countries are adopting it quickly to stop using ethylene oxide. It's very efficient

You don't need a lot to make it work

A modified vaccume chamber. A oxygen concentrator. A ozone generator. (Quartz generator that accepts pressured air). And various tubing that can withstand the ozone. The hydrogen vapor can be be generated using a ultra sonic humidifier before hand. Run it in the chamber to saturate it. Concentration can be 15%. Treat it exactly like an autoclave cycle. Except instead of pressure your doing vac after you push some ozone in there in cycle of 5 psi in for 5 mins. Then pull a vac. The. Release. Push more ozone. Do it 3 times. This will displace all the air with only ozone even in filter paper


It sounds convoluted but it's just turning a few levers and switches.....but the venting part is trick I run a tube outside so it vents out and not into my lab.....ozone at this high of concentration makes you light ahead and you will get deadly sick. But a vent with a tube is enough to fix that.



The beauty of this method is it needs zero heat. Yet it works as an entire sterilization cycle and yes dental paper back autoclave bags are compatible!


It's also faster than autoclave and no drying cycle.

What Items are you using it for ? I don't really have much that can't takes steam or dry heat. Except disposable stuff. All.glass and stainless get 250°C for 45 min for depyro and everything else bagged in steam. I looked into EtO but thats shit is expensive and high cancer risk. And pain in the ass to get the gas canisters. And H202 vapor is too dangerous for a home lab.

 

[Walter Hype]

What Items are you using it for ? I don't really have much that can't takes steam or dry heat. Except disposable stuff. All.glass and stainless get 250°C for 45 min for depyro and everything else bagged in steam. I looked into EtO but thats shit is expensive and high cancer risk. And pain in the ass to get the gas canisters. And H202 vapor is too dangerous for a home lab.

I use it for everything now


It's compatible with basically everything that is the ideal version of what we're doing glass stainless steel and PTFE

That's generally what I would use it for you can use it on most disposables if it's paperback but it's not compatible with tyvek


You can look up a list of what it's compatible and what's long-term certain items are basically one-time use and other items can be multiple cycles

My primary use case for this is getting my filter paper to be endotoxin free a lot of the filter paper that is pre sterilized are due to gamma sterilization or ethylene oxide. Both of which we do not have access to

However we can get as close as possible with ozone sterilization you don't necessarily need the hydrogen peroxide vapor however it is said to make the reaction even more oxidative and even more strong towards endotoxins however you can easily overdo it with just overdosing with more ozone rather than using the hydrogen the hydrogen just cuts down the time required


So you could technically run your entire filter apparatus in an autoclave bag with just way more ozone than necessary and it will still achieve the same result

The hydrogen vapor just makes it way easier for endotoxins to break down

The vapor is not dangerous you can literally use a off-the-shelf ultrasonic vapor generator for babies

All its really doing is increasing the humidity and the oxidative power of ozone. It's just very synergistic


I too care about sterility and trying to achieve pharmaceutical grade sterility and that's been my goal for months

Ozone sterilization technology has been basically my focus. I've done a lot of modification to a vacuum chamber trying to copy the patents that the FDA approved on. I truly think it's the most accessible tech currently that could change what we think is possible for safety and sterile. Nothing comes close except gamma........but how many here has access to that? ...this is actually accessible and I suspect more people will copy this soon enough.


I'm always about finding things that are actually accessible and not gate kept

I absolutely despise gatekeeping in any capacity

Knowledge if needed should be freely available. We are in the stonge ages here in the ugl and it's because we are not able to pin point a path forward due to so many methods and gate keeping.

Ozone I believe is a level up above the rest. All the benefits of gas treatment..but it's not regulated and can be powerful

 

[Motojager]

I have a local lab that hooks me up on the charles river. I get the 2001f and 2005f for cheaper than you can buy the unlicenced for. I do manual test I get the FTM and TSB in vials and run membrane test and drop in then incubate. I sourced some Croda super refined polysorbate 20 so I can do finished oils. That was a bitch to get. Why they regulate it so hard is crazy to me. Simple process though vortex and centrifuge with the PS20.

 

[Thorshammer]

For only pyrogen load on the filter membrane alone. Im already passing each batch with no gel clot test. The ones under the standard for IV bags. You use anything like this to validate it. I use the 34% H202 but not airborne with sterile clean room wipes all inside my hood for an hour to oxidize the work area. Breathing that would be bad.

 

[Motojager]

I use it for everything now


It's compatible with basically everything that is the ideal version of what we're doing glass stainless steel and PTFE

That's generally what I would use it for you can use it on most disposables if it's paperback but it's not compatible with tyvek


You can look up a list of what it's compatible and what's long-term certain items are basically one-time use and other items can be multiple cycles

My primary use case for this is getting my filter paper to be endotoxin free a lot of the filter paper that is pre sterilized are due to gamma sterilization or ethylene oxide. Both of which we do not have access to

However we can get as close as possible with ozone sterilization you don't necessarily need the hydrogen peroxide vapor however it is said to make the reaction even more oxidative and even more strong towards endotoxins however you can easily overdo it with just overdosing with more ozone rather than using the hydrogen the hydrogen just cuts down the time required


So you could technically run your entire filter apparatus in an autoclave bag with just way more ozone than necessary and it will still achieve the same result

The hydrogen vapor just makes it way easier for endotoxins to break down

The vapor is not dangerous you can literally use a off-the-shelf ultrasonic vapor generator for babies

All its really doing is increasing the humidity and the oxidative power of ozone. It's just very synergistic


I too care about sterility and trying to achieve pharmaceutical grade sterility and that's been my goal for months

Ozone sterilization technology has been basically my focus. I've done a lot of modification to a vacuum chamber trying to copy the patents that the FDA approved on. I truly think it's the most accessible tech currently that could change what we think is possible for safety and sterile. Nothing comes close except gamma........but how many here has accept to that? ...this is actually accessable and I suspect more people will copy this soon enough.

So you are going to take the time to ozone something yet you just slap a membrane in the top of a buchner and just run product around it? Makes zero sense to me.

 

[Walter Hype]

So you are going to take the time to ozone something yet you just slap a membrane in the top of a buchner and just run product around it? Makes zero sense to me.

I use a stainless steel clasp style funnel that contains a gasket that prevents bypass. I guess I haven't updated you with my changes over these months. I no longer use the glass style

OMICRON® FFSSN47 Stainless Steel 316 Membrane Filter Holder, 47mm Diameter, 300mL Capacity | LabX.com

OMICRON® FFSSN47 Stainless Steel 316 Membrane Filter Holder, 47mm Diameter, 300mL Capacity - New - $189.00 USD from Omicron Scientific Inc

www.labx.com

I use this. I have 5 of them. I swap as needed


I'm working on an even large one that accepts 90mm or 140 mm style. Same clasp mechanism. I have talked to the manufacturer directly and they have confirmed bypass is not possible and this is fully autoclaveble with filter paper.

 

[Motojager]

I use a stainless steel clasp style funnel that contains a gasket that prevents bypass. I guess I haven't updated you with my changes over these months. I no longer use the glass style

OMICRON® FFSSN47 Stainless Steel 316 Membrane Filter Holder, 47mm Diameter, 300mL Capacity | LabX.com

OMICRON® FFSSN47 Stainless Steel 316 Membrane Filter Holder, 47mm Diameter, 300mL Capacity - New - $189.00 USD from Omicron Scientific Inc

www.labx.com

I use this. I have 5 of them. I swap as needed

About fucking time.

 

[Walter Hype]

For only pyrogen load on the filter membrane alone. Im already passing each batch with no gel clot test. The ones under the standard for IV bags. You use anything like this to validate it. I use the 34% H202 but not airborne with sterile clean room wipes all inside my hood for an hour to oxidize the work area. Breathing that would be bad.

Can we talk in DM and I send you a sample to run ? I'll pay you. Don't worry I'll use drop addy and all that full opsec stuff. Just wanna test something.

 

[Walter Hype]

About fucking time.

Yeah I like that it won't break. My funnels kept breaking so I gave up using them. One bumb and they crack.

 

[Thorshammer]

Yeah I like that it won't break. My funnels kept breaking so I gave up using them. One bumb and they crack.

I have a bunch of those I just clamp the filter in them stick in a self seal bags and autoclave the whole thing with the inline air filter and last section of tubing.

 

[Walter Hype]

I have a bunch of those I just clamp the filter in them stick in a self seal bags and autoclave the whole thing with the inline air filter and last section of tubing.

did you just say inline air filter? mind showing me what your saying? this is new to me....im going to assume you mean the top of the funnel. i always wondered wtf that was for. pressure ? figured it could do vacuum and also do pressure filtration with tubing. but yeah i use these funnels and ozone the crap out of it then i take the same bag and autoclave it too

 

[Thorshammer]

did you just say inline air filter? mind showing me what your saying? this is new to me....im going to assume you mean the top of the funnel. i always wondered wtf that was for. pressure ? figured it could do vacuum and also do pressure filtration with tubing. but yeah i use these funnels and ozone the crap out of it then i take the same bag and autoclave it too

No a barbed 50mm PTFE hydrophobuc air filter from a short peice of silicon vacuum hose to the flask. Or when you disconnect the hose you have a vacuum sucking in unsterile air.

 

[Thorshammer]

This

 

[Walter Hype]

No a barbed 50mm PTFE hydrophobuc air filter from a short peice of silicon vacuum hose to the flask. Or when you disconnect the hose you have a vacuum sucking in unsterile air.

ahhh ok now i get it. this makes me think where do you find 50 mm air filters? now im scrambling to check mine. iv always found 33mm the max size i could fine ..wait you said inline nvm iv seen those. smart!

 

[Walter Hype]

This

ok i get it now...thats hella smart its big enough to not slow down vac but also keeps air sterile going in..,..nice! def going to use this idea if you dont mind

 

[Thorshammer]

Amazon they are touch you can autoclave them like 10 times each.

 

[Motojager]

Amazon they are touch you can autoclave them like 10 times each.

What are your funnel setups and pump setups y’all using? I have a couple 47mm the are ddidica

 

[Thorshammer]

My pump is tried to a cold trap and 4 way with desicator, vacuum oven and rotovap with an extra long hose for filtration and the exaughst goes into the fume hood. Some batches I use a stainless reusable 50mm clamp set up with that peristaltic pump . Depends what the use case.

 

[Walter Hype]

My pump is tried to a cold trap and 4 way with desicator, vacuum oven and rotovap with an extra long hose for filtration and the exaughst goes into the fume hood. Some batches I use a stainless reusable 50mm clamp set up with that peristaltic pump . Depends what the use case.

I personally use a rocker PTFE pump mostly because it was one of the most robust pumps I could possibly find for the price that can withstand almost anything you throw at it


If you go on eBay for example there's tons of companies throwing them out due to small issues that they don't really really understand how to fix


I got a brand new one that's worth 700 bucks and I got it for $150 turns out they didn't know how to tighten the pumps piston head all it was was a few screws loose

I got a brand new pump that can stand the test of time all because I know how to deal hunt


This thing can pull a vacuum so damn fast and it can handle moisture chemicals whatever you throw out it all because all the wetted components are PTFE